本文選題：硒代蛋氨酸　切入點：脂多糖　出處：《動物營養(yǎng)學(xué)報》2017年09期

【摘要】：本試驗旨在研究硒(Se)對脂多糖(LPS)誘導(dǎo)的奶牛乳腺上皮細胞(BMEC)氧化損傷的保護作用及其機制。將貼壁生長的第3代BMEC隨機分為8組,每組6個重復(fù),每個重復(fù)1個培養(yǎng)孔。對照(CON)組采用基礎(chǔ)培養(yǎng)液,不添加Se和LPS,培養(yǎng)30h;LPS組和6個Se保護組在基礎(chǔ)培養(yǎng)液中分別添加不同水平的Se(0、10、20、50、100、150和200nmol/L),培養(yǎng)24h后,加入1μg/mL LPS作為外源刺激作用6h。結(jié)果表明:1)與CON組相比,LPS組BMEC的相對增殖率顯著下降(P0.05),谷胱甘肽過氧化物酶(GPx)、硫氧還蛋白還原酶(TrxR)、總超氧化物歧化酶(T-SOD)、過氧化氫酶(CAT)活性和總抗氧化能力(T-AOC)均顯著下降(P0.05),GPx1和TrxR1的基因和蛋白表達量、硒蛋白P(SelP)含量也顯著下調(diào)(P0.05);而LPS組的一氧化氮(NO)含量,誘導(dǎo)型一氧化氮合酶(iNOS)活性及其基因和蛋白表達量,炎癥因子腫瘤壞死因子-α(TNF-α)、白介素-1(IL-1)和白介素-6(IL-6)含量及其基因表達量,活性氧(ROS)活性,丙二醛(MDA)含量均顯著升高(P0.05),絲裂原活化蛋白激酶(MAPK)信號通路相關(guān)因子p38絲裂原活化蛋白激酶(p38 MAPK)、c-Jun氨基端激酶(JNK)、細胞外信號調(diào)節(jié)激酶1/2(ERK1/2)的基因表達量呈相似變化。2)與LPS組相比,Se保護組隨Se添加水平的增加,相對增殖率,T-SOD、CAT、GPx、TrxR活性,T-AOC,GPx1、TrxR1基因和蛋白表達量均呈先升高后下降趨勢;而NO含量,iNOS活性及其基因和蛋白表達量,炎癥因子TNF-α、IL-1、IL-6含量及其基因表達量,MAPK信號通路相關(guān)因子ERK1/2、JNK、p38 MAPK的基因表達量,ROS活性,MDA含量呈先降低后升高的趨勢;以20~100nmol/L Se保護效果較好,綜合來看50nmol/L Se保護效果最好。結(jié)果提示,Se可提高BMEC的抗氧化功能,對LPS引起的細胞氧化損傷具有保護作用,其機制是Se增強TrxR活性從而抑制MAPK信號通路的激活,最終減少NO的大量釋放,但過高水平的Se會對細胞造成損傷。培養(yǎng)液中20~100nmol/L Se的保護作用較好,尤其以50nmol/L Se效果最好。
[Abstract]:The aim of this study was to investigate the protective effect of selenoside on oxidative damage induced by lipopolysaccharide (LPS) in dairy cow mammary epithelial cells (BMECs) and its mechanism. The third passage BMEC of adherent growth was randomly divided into 8 groups with 6 replicates in each group. The control group was treated with basic culture medium without se and LPS.The 30 h LPS group and 6 se protection group were cultured with different levels of Seo 01020D 50100150 and 200nmol / L 1, respectively, and cultured for 24 h. Addition of 1 渭 g/mL LPS as exogenous stimulator for 6 h. The results showed that the relative proliferation rate of BMEC in CON group was significantly lower than that in CON group (P 0.05), glutathione peroxidase (Glutathione peroxidase), thioredoxin reductase (TRA), total superoxide dismutase (T-SOD), catalase. The activity of CAT and total antioxidant capacity (T-AOC) significantly decreased the gene and protein expression of P0.05, GPx1 and TrxR1. In LPS group, the content of nitric oxide (no), the activity of inducible nitric oxide synthase (iNOS), its gene and protein expression, the content of inflammatory factor tumor necrosis factor- 偽 (TNF- 偽), interleukin-1 (IL-1) and interleukin-6IL-6 (IL-6) and its gene expression were also significantly down-regulated. Reactive oxygen species (Ros) activity, The content of malondialdehyde (MDA) MDAs increased significantly (P 0.05). The expression of p38 mitogen-activated protein kinase (p38 MAPK) signal pathway associated with c-Jun amino terminal kinase (JNKK) and extracellular signal-regulated kinase 1 / 2 ERK1 / 2 were similar. Compared with the LPS group, the se protection group increased with the increase of se addition level. The relative proliferative rate and the activity of T-SOD CATX TXR gene and protein increased at first and then decreased, while the no content and the activity of iNOS and its gene and protein expression showed a tendency to increase first, and then to decrease, while the activity of iNOS and the expression of gene and protein in the activity of T-AOCU GPx1 + TrxR1 showed a tendency to increase first and then to decrease. The content of IL-6 and its gene expression in inflammatory factor TNF- 偽 and its gene expression; the gene expression of ERK1 / 2 MAPK and Ros activity were decreased first and then increased, and the protective effect of 20~100nmol/L se was better. The results suggest that se can enhance the antioxidant function of BMEC and protect cells from oxidative damage induced by LPS. The mechanism is that se enhances the activity of TrxR and inhibits the activation of MAPK signaling pathway. In the end, the release of no was reduced, but too high level of se could damage the cells. The protective effect of 20~100nmol/L se in culture medium was better, especially 50nmol/L se was the best.
【作者單位】： 內(nèi)蒙古農(nóng)業(yè)大學(xué)動物科學(xué)學(xué)院;
【基金】：國家自然科學(xué)基金(31560650)
【分類號】：S823.5

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