本文選題：牦牛　切入點：生殖器官　出處：《甘肅農(nóng)業(yè)大學(xué)》2015年碩士論文

【摘要】：為了探討牦牛腎臟中黃體生成素受體(luteinizing hormone receptor,LHR)的表達情況及其與卵巢中LHR表達的相似性,本研究檢測了LHR在牦牛腎臟和卵巢中的表達量。為了探討LHR基因在牦牛發(fā)情周期不同階段對其生殖器官的調(diào)節(jié)作用,本研究檢測了LHR基因在牦牛發(fā)情周期不同階段生殖系統(tǒng)中的表達差異,并分析了出現(xiàn)差異變化的原因。研究參照黃牛LHR基因序列、牦牛β-actin基因序列和GAPDH基因序列設(shè)計特異性引物。選取健康的雌性牦牛為研究對象,利用實時熒光定量PCR(RT-qPCR)法分析牦牛腎臟和卵巢組織中LHR基因的相對表達量,并運用免疫組織化學(xué)法對LHR蛋白在牦牛腎臟和卵巢組織中進行表達和定位研究。選取健康的處于卵泡期和黃體期的雌性牦牛為研究對象,利用RT-qPCR法分析牦牛發(fā)情周期不同階段生殖系統(tǒng)中LHR基因的相對表達量。研究結(jié)果表明:(1)牦牛腎臟具有表達LHR的特性,其LHR基因的相對表達量是卵巢的79.75倍。(2)LHR在牦牛腎臟組織中的近曲小管和遠曲小管呈陽性反應(yīng),在卵巢組織中的卵泡顆粒層呈明顯的陽性反應(yīng),在牦牛腎臟和卵巢中的表達差異極顯著(P0.01)。(3)LHR基因在牦牛發(fā)情周期不同階段生殖系統(tǒng)中的表達量存在差異,卵巢和輸卵管中卵泡期LHR基因的表達量高于黃體期,子宮中LHR基因的表達量黃體期高于卵泡期。從本研究可以看出,牦牛的腎臟組織與卵巢組織一樣,均具有表達LHR的特性,提示牦牛腎臟可通過調(diào)節(jié)LHR參與其繁殖性能。牦牛在發(fā)情周期不同階段生殖系統(tǒng)中LHR基因的表達量存在差異,提示LHR在牦牛的繁殖過程中對生殖系統(tǒng)具有重要的調(diào)節(jié)作用。
[Abstract]:To investigate the expression of luteinizing hormone receptor (LHR) in yak kidney and its similarity with LHR expression in ovary. In this study, we examined the expression of LHR in the kidney and ovary of yak. In order to investigate the regulation of LHR gene on the reproductive organs of yak at different stages of estrous cycle, The purpose of this study was to detect the difference of LHR gene expression in the reproductive system of yak at different stages of estrous cycle, and to analyze the causes of the difference. Specific primers were designed for 尾 -actin gene sequence and GAPDH gene sequence of yak. The relative expression of LHR gene in kidney and ovary of yak was analyzed by real-time fluorescence quantitative PCR RT-qPCRmethod. The expression and localization of LHR protein in the kidney and ovary of yak were studied by immunohistochemical method. The healthy female yak in follicular and luteal phase was selected as the study object. RT-qPCR method was used to analyze the relative expression of LHR gene in the reproductive system of yak at different stages of estrous cycle. The results showed that the kidney of yak had the characteristic of expressing LHR. The relative expression of LHR gene was 79.75 times higher than that of ovaries. The proximal tubules and distal convoluted tubules in the kidney tissues of yak showed positive reaction, and the follicular granular layers in the ovarian tissues showed obvious positive reaction. There were significant differences in the expression of P0.01LHR gene in yak kidney and ovaries. The expression of LHR gene in oviducts and oviducts was higher than that in luteal phase in different stages of oestrous cycle of yak, and the expression of P0.01LHR gene in oviduct and oviduct was higher than that in luteal phase. The expression of LHR gene in uterus was higher in luteal phase than in follicular phase. The results suggested that the kidney of yak could participate in the reproductive performance by regulating LHR. There were differences in the expression of LHR gene in the reproductive system of yak at different stages of estrous cycle, suggesting that LHR plays an important role in regulating the reproductive system of yak.
【學(xué)位授予單位】：甘肅農(nóng)業(yè)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：S823.85

【參考文獻】

相關(guān)期刊論文 前2條

1 牛淑玲,豐培金,周虛;促黃體素受體研究進展[J];動物醫(yī)學(xué)進展;2003年05期

2 張哲;鄭清蓮;劉永惠;;去卵巢大鼠腎臟組織表達FSHR和LHR[J];基礎(chǔ)醫(yī)學(xué)與臨床;2012年10期

，

本文編號：1683792