本文選題：共軛亞油酸　切入點(diǎn)：紅細(xì)胞數(shù)量　出處：《河南農(nóng)業(yè)大學(xué)》2015年碩士論文

【摘要】：共軛亞油酸(CLA)是一種常用的食品添加劑,但是動(dòng)物試驗(yàn)表明日糧中添加CLA會(huì)造成明顯的代謝障礙,如脂肪肝、高血糖等。同時(shí)CLA是一種不飽和脂肪酸,可對(duì)紅細(xì)胞膜脂質(zhì)雙層造成一定的影響。已有研究表明,給老年大鼠飼喂添加CLA的飼料可使紅細(xì)胞膜總n-6不飽和脂肪酸含量顯著降低,n-6/n-3比值會(huì)下降,而這種變化可能對(duì)紅細(xì)胞膜流動(dòng)性、滲透脆性及溶血速率產(chǎn)生不良影響。但是,飼喂添加CLA的飼料對(duì)紅細(xì)胞數(shù)量的影響尚未見報(bào)道。CLA可導(dǎo)致小鼠代謝障礙,這在本實(shí)驗(yàn)室先前的研究中已有報(bào)道。本論文在CLA導(dǎo)致小鼠代謝障礙的情況下,對(duì)紅細(xì)胞數(shù)量的改變及其可能的原因進(jìn)行了研究。首先,給39日齡昆明白雄性小鼠飼喂含1.5%共軛亞油酸的飼料(CLA組,n=8),以1.5%的亞油酸為對(duì)照組(LA組,n=8),CLA組小鼠表現(xiàn)出脂肪肝和脂肪組織減少為特征的代謝障礙。然后,檢測(cè)兩組小鼠RBC(紅細(xì)胞計(jì)數(shù))、HGB(血紅蛋白濃度)、HCT(紅細(xì)胞壓積)、MCV(平均紅細(xì)胞體積),結(jié)果顯示,與LA組相比,CLA組小鼠RBC減少了48.96%,HCT減少了46.49%,MCV升高了7.82%,差異均顯著(P0.01),CLA組小鼠HGB有降低的趨勢(shì)(差異不顯著)。連續(xù)檢測(cè)發(fā)現(xiàn),在飼喂的第47,61,86天,紅細(xì)胞數(shù)量較LA組減少,差異顯著(P0.05)。CLA組小鼠的血液網(wǎng)織紅細(xì)胞數(shù)量是LA組的兩倍多(P0.01)。此結(jié)果提示CLA組小鼠骨髓造血功能代償性增強(qiáng)。本論文又對(duì)日糧中CLA降低小鼠紅細(xì)胞數(shù)量的機(jī)制進(jìn)行了探討。首先,我們檢測(cè)了紅細(xì)胞膜的滲透脆性,結(jié)果發(fā)現(xiàn)CLA組小鼠與LA組小鼠之間并無顯著變化(P0.05)。隨后,Q-PCR法檢測(cè)小鼠腎臟EPO基因表達(dá)量以及亞鐵嗪比色法檢測(cè)血清鐵含量,發(fā)現(xiàn)CLA組小鼠與LA組間并無顯著差異,但是ELASA試劑盒檢測(cè)VB12、葉酸含量發(fā)現(xiàn),CLA組小鼠的血漿VB12含量較LA組小鼠減少了24.72%(P0.05),血漿中葉酸含量則減少了23.15%(P0.01)。并且,血常規(guī)檢測(cè)結(jié)果顯示,與LA組相比,CLA組小鼠的單核細(xì)胞比例(MONO%)升高了41.77倍(P0.05)。上述結(jié)果提示,CLA組小鼠紅細(xì)胞數(shù)量減少的原因可能是紅細(xì)胞合成原料(VB12和葉酸)不足,或單核細(xì)胞吞噬紅細(xì)胞的作用加強(qiáng),或者兩者都是。結(jié)論:日糧中CLA使小鼠紅細(xì)胞數(shù)量減少;血漿中VB12和葉酸含量降低以及MONO%升高可能是紅細(xì)胞數(shù)量減少的原因;CLA組網(wǎng)織紅細(xì)胞數(shù)量增多代表了骨髓造血代償性增強(qiáng)。
[Abstract]:Conjugated linoleic acid (CLA) is a common food additive, but animal experiments have shown that dietary CLA can cause obvious metabolic disorders, such as fatty liver, hyperglycemia, etc. CLA is also a kind of unsaturated fatty acid. It has been shown that the content of total n-6 unsaturated fatty acids in erythrocyte membrane decreased significantly in aged rats fed with CLA, and the ratio of n-6 / n-3 decreased significantly. This change may have a negative effect on erythrocyte membrane fluidity, osmotic fragility and hemolysis rate. However, the effect of diet supplemented with CLA on erythrocyte number has not been reported. This has been reported in previous studies in our laboratory. In this study, changes in erythrocyte count and their possible causes were studied in mice with metabolic disorders caused by CLA. Kunming white mice were fed with 1.5% conjugated linoleic acid (CLA) diet for 39 days, and 1.5% linoleic acid was used as control group. The mice in the LA group showed metabolic disorders characterized by fatty liver and adipose tissue reduction. The RBC (erythrocyte count) HGB( hemoglobin concentration) HCT (mean erythrocyte volume) in the two groups were detected. Compared with LA group, RBC decreased by 48.96% and 46.49%, and increased 7.82%. The difference was significant (P 0.01) compared with LA group (the difference was not significant). Continuous test showed that the number of red blood cells was lower than that of LA group on the 47th day, 6186 days after feeding, the number of RBC was lower than that of LA group. The number of reticulocyte in the CLA group was more than twice that in the LA group. The results suggested that the bone marrow hematopoietic function of the CLA group was compensatory. The mechanism of CLA decreasing the erythrocyte count in the diet was also discussed in this paper. The osmotic fragility of erythrocyte membrane was detected. The results showed that there was no significant change between CLA group and LA group. Subsequently, the expression of EPO gene in kidney of mice was detected by Q-PCR and the serum iron content was detected by ferrizine colorimetry. It was found that there was no significant difference between CLA group and LA group, but VB12 was detected by ELASA kit. Compared with LA group, the content of plasma VB12 decreased 24.72% (P 0.05), and the content of plasma folic acid decreased 23.15% (P 0.01). Compared with LA group, monocyte ratio in CLA group was 41.77 times higher than that in LA group (P 0.05). These results suggested that the decrease of erythrocyte number in CLA group might be due to the deficiency of VB12 and folic acid, or the enhancement of monocyte phagocytosis. Conclusion: CLA in diet can reduce the number of red blood cells in mice. The decrease of plasma levels of VB12 and folic acid and the increase of mono% may be the reason for the decrease of erythrocyte number. The increase of reticulocyte represents the enhancement of hematopoiesis compensability in bone marrow.
【學(xué)位授予單位】：河南農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：S816

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