發(fā)布時間：2018-03-28 11:03

  本文選題：日本腦炎病毒　切入點：分離鑒定　出處：《中國獸醫(yī)科學》2017年01期

【摘要】：為了解上海市蚊源日本腦炎病毒(JEV)的分子特征,2014年從上海市嘉定區(qū)豬場采集蚊媒樣品1萬余份,采用BHK21和C6/36細胞培養(yǎng)法進行病毒分離,通過RT-PCR和IFA技術(shù)對分離株進行鑒定。結(jié)果表明,分離到1株JEV,命名為JD-15,源自中華按蚊。用JD-15株分別感染BHK21和C6/36細胞,72 h后所有細胞均發(fā)生明顯病變(CPE),其細胞半數(shù)感染量(TCID50)為1.96×106PFU。用分離株JD-15腦內(nèi)接種3周齡乳鼠,結(jié)果測得半數(shù)致死量(LD50)為4.2×101PFU,表明該毒株具有較強的神經(jīng)毒力。對JD-15株的E基因進行克隆測序及進化分析,結(jié)果顯示,該株JEV屬于基因Ⅲ型,與減毒活疫苗株SA-14-14-2的E基因核苷酸序列的同源性為98.1%,氨基酸序列的同源性為97.5%。本研究為乙型腦炎的流行病學調(diào)查提供了科學依據(jù),對上海市蚊蟲及蚊媒病的防治具有重要意義。
[Abstract]:In order to understand the molecular characteristics of Japanese encephalitis virus (JEV) from Shanghai mosquitoes, more than 10 000 mosquito vector samples were collected from Jiading District Pig Farm in 2014. The virus was isolated by BHK21 and C6 / 36 cell culture. The isolates were identified by RT-PCR and IFA. A strain of JEV, named JD-15, was isolated from Anopheles sinensis. BHK21 and C6 / 36 cells were infected with JD-15 strain for 72 h, respectively. Results LD50 was 4.2 脳 10 ~ (-1) PFU, which indicated that the strain had strong neurotoxicity. The E gene of JD-15 strain was cloned and sequenced. The result showed that the strain JEV belonged to type 鈪，

本文編號：1676052