本文選題：麥洼牦牛　切入點(diǎn)：GH基因　出處：《畜牧獸醫(yī)學(xué)報(bào)》2017年04期

【摘要】：旨在探討生長(zhǎng)激素(Growth hormone,GH)、生長(zhǎng)激素受體(Growth hormone receptor,GHR)、生長(zhǎng)激素促分泌素受體(Growth hormone secretagogue receptor,GHSR)基因在麥洼牦牛中的遺傳多樣性,揭示不同基因型與其生長(zhǎng)性狀的關(guān)聯(lián)性,同時(shí)為候選基因在牦牛中的表達(dá)調(diào)控提供理論依據(jù),尋找可用于遺傳育種輔助選擇的分子標(biāo)記。本試驗(yàn)采用DNA池技術(shù),結(jié)合PCR-RFLP和直接測(cè)序法研究麥洼牦牛GH、GHR、GHSR基因的遺傳多態(tài)性,分析候選基因多態(tài)位點(diǎn)與體高、體斜長(zhǎng)、胸圍、管圍和體重等生長(zhǎng)性狀的關(guān)聯(lián)性。結(jié)果表明:1)麥洼牦牛GH、GHR、GHSR基因均存在多態(tài)性,其中GH基因存在A757G和T949C2個(gè)SNPs位點(diǎn),GHR基因發(fā)現(xiàn)T2416C、T3490C和A7500G3個(gè)突變位點(diǎn);GHSR基因存在T1387C和T3006C突變;2)適合性檢驗(yàn)表明,GHR基因A7500G位點(diǎn)在粉嘴類群,及GHSR基因T3006C位點(diǎn)在純黑類群中偏離Hardy-Weinberg平衡狀態(tài),其他位點(diǎn)均符合Hardy-Weinberg平衡;3)差異顯著性檢驗(yàn)表明,GHR基因T2416C、T3490C和A7500G位點(diǎn)與麥洼牦牛管圍極顯著相關(guān)(P0.01),GHSR基因T1387C位點(diǎn)與其體重顯著相關(guān)(P0.05)。麥洼牦牛GH、GHR、GHSR基因均存在遺傳多態(tài)性,推斷GHR基因T2416C、T3490C和A7500G位點(diǎn)、GHSR基因T1387C位點(diǎn)可能是影響麥洼牦牛管圍、體重性狀的主基因或與主基因相連鎖的基因座,可作為輔助選擇的遺傳標(biāo)記。
[Abstract]:The aim of this study was to investigate the genetic diversity of growth hormone growth hormone secretagogue receptor GHSRgene in Maiwa Yak, and to reveal the relationship between different genotypes and their growth traits. At the same time, it provides a theoretical basis for the regulation of candidate gene expression in yak, and looks for molecular markers that can be used for genetic and breeding assisted selection. The genetic polymorphism of the GHR-GHSR gene of GHHHR-GHSR gene was studied by DNA pool technique, PCR-RFLP and direct sequencing in Mai WA Yak. The relationship between candidate gene polymorphism and body height, body length, chest circumference, tube circumference and body weight was analyzed. The results showed that the GHR-GHSR gene was polymorphic in GHHR-GHSR gene of Maiwa Yak. Among them, there were A757G and T949C2 SNPs loci in GH gene. T2416CnT3490C and A7500G3 GHSR gene had T1387C and T3006C mutations. And the GHSR gene T3006C locus deviated from the Hardy-Weinberg equilibrium in the pure black group. All the other loci were consistent with Hardy-Weinberg balance 3 (P < 0.05). The results showed that there was a significant correlation between GHR T2416CnT3490C and A7500G loci and the tube circumference of Mai WA Yak (P0.01GHSR gene was significantly correlated with its body weight (P0.05). The genetic polymorphism of GHGHR-GHSR gene was found in Maiwa Yak. It is inferred that GHR gene T2416CfT3490C and A7500G locus T1387C may be the main genes affecting the tube circumference and body weight of Mai WA yak, or the loci linked to the main genes, which can be used as genetic markers for auxiliary selection.
【作者單位】： 西南民族大學(xué)青藏高原研究院;西南民族大學(xué)動(dòng)物遺傳育種學(xué)國(guó)家民委-教育部重點(diǎn)實(shí)驗(yàn)室;西南民族大學(xué)青藏高原生態(tài)保護(hù)與畜牧業(yè)高科技研究示范基地;
【基金】：四川省科技計(jì)劃項(xiàng)目(2015JY0248) 中央高�；究蒲许�(xiàng)目(2016NZYQN30) 國(guó)家肉牛牦牛產(chǎn)業(yè)技術(shù)體系(2017)
【分類號(hào)】：S823.85

【相似文獻(xiàn)】

相關(guān)期刊論文 前2條

1 單雪松;陳洋;崔慶媛;李克鵬;楊雨江;;北京鴨×番鴨雜種優(yōu)勢(shì)差異表達(dá)基因GHR的克隆[J];中國(guó)獸醫(yī)學(xué)報(bào);2010年03期

2 ;[J];;年期

相關(guān)會(huì)議論文 前1條

1 宋興超;楊福合;巴恒星;邢秀梅;魏海軍;;梅花鹿生長(zhǎng)激素受體(GHR)基因完整編碼區(qū)序列生物信息學(xué)分析[A];2012中國(guó)鹿業(yè)進(jìn)展[C];2012年

相關(guān)博士學(xué)位論文 前1條

1 杜智恒;北極狐GH、GHR、IGF1和IGF1R基因的cDNA克隆、表達(dá)及相關(guān)性狀的研究[D];東北農(nóng)業(yè)大學(xué);2008年

相關(guān)碩士學(xué)位論文 前5條

1 楊云蔚;GHR、FOXC2、HO-1基因與云南地區(qū)漢族、哈尼族OSAHS發(fā)病的多態(tài)性研究[D];昆明醫(yī)科大學(xué);2015年

2 顧陽(yáng);西藏小型豬GH、GHR、IGF-1基因的多態(tài)性及其與生長(zhǎng)性狀的相關(guān)性分析[D];南方醫(yī)科大學(xué);2013年

3 張浩;甘肅肉羊新品種群GH、GHR & Leptin遺傳變異及其與體重的相關(guān)分析[D];甘肅農(nóng)業(yè)大學(xué);2010年

4 喬西波;家兔GH、GHR及MSTN基因多態(tài)性及其與產(chǎn)肉性狀的關(guān)系[D];山東農(nóng)業(yè)大學(xué);2010年

5 李建升;賴氨酸對(duì)綿羊GHR和IGF-1基因表達(dá)調(diào)控的影響[D];甘肅農(nóng)業(yè)大學(xué);2009年

，

本文編號(hào)：1668339