本文選題：鴨　切入點(diǎn)：種鴨場(chǎng)　出處：《黑龍江畜牧獸醫(yī)》2017年04期

【摘要】：為了解種鴨場(chǎng)鴨舍內(nèi)外環(huán)境中細(xì)菌的種類、數(shù)量及分布情況,采用菌落計(jì)數(shù)、細(xì)菌分離培養(yǎng)、革蘭氏染色、生化試驗(yàn)及16S rRNA鑒定等方法對(duì)山東省濰坊地區(qū)某種鴨場(chǎng)鴨舍內(nèi)外的空氣、墻壁、地面、水槽和料槽樣品進(jìn)行了檢測(cè)。結(jié)果表明:鴨舍內(nèi)空氣中細(xì)菌菌落總數(shù)為3 921 cfu/m~3,明顯高于舍外空氣中細(xì)菌總數(shù);不同物體表面細(xì)菌的數(shù)量不同,墻壁細(xì)菌總數(shù)為123 cfu/cm~2,地面細(xì)菌總數(shù)為4 342 cfu/cm~2,水槽細(xì)菌總數(shù)為2 579 cfu/cm~2,料槽細(xì)菌總數(shù)為1 542 cfu/cm~2。從117份樣品中分離到106株細(xì)菌,其中芽胞桿菌37株,占檢出菌的34.9%(37/106);葡萄球菌30株,占檢出菌的28.3%(30/106);腸桿菌30株,占檢出菌的28.3%(30/106);鏈球菌9株,占檢出菌的8.5%(9/106),且空氣中和地面表面的細(xì)菌種類最多。說明種鴨場(chǎng)鴨舍內(nèi)外環(huán)境中葡萄球菌、芽胞桿菌和腸桿菌是主要污染菌。
[Abstract]:In order to understand the types of bacteria to duck duck breed farms in internal and external environment, the number and distribution of the colony count, bacterial culture, Gram staining, biochemical test and 16S rRNA identification method in Shandong area of Weifang Province, a duck duck inside and outside air, wall, ground, sink and trough samples duck. The results show that the indoor air total bacterial count was 3921 cfu/m~3, significantly higher than the total number of bacteria in the air of homes; the number of bacteria on the surface of different objects are different, the total number of bacteria in the wall is 123 cfu/cm~2, the total number of bacteria in the ground for 4342 cfu/cm~2, the total number of bacteria in water is 2579 cfu/cm~2, the total number of bacteria in the trough of 1542 cfu/cm~2. isolated from 117 samples of 106 strains of bacteria, including Bacillus 37 strains, accounting for 34.9% of the detected bacteria (37/106); 30 strains of Staphylococcus bacteria accounted for 28.3% (30/106); intestinal bacteria of 30 strains, accounting for the detection of bacteria in 28.3% (30/106) 9 strains of Streptococcus accounted for 8.5% (9/106) of the detected bacteria, and the most kinds of bacteria were found in the air and the ground surface. It indicated that Staphylococcus, bacillus and Enterobacteriaceae were the main contaminating bacteria in the duck farm inside and outside the duck farm.

【作者單位】： 山東畜牧獸醫(yī)職業(yè)學(xué)院;
【分類號(hào)】：S852.61;;S852.65+7

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本文編號(hào)：1658160