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  本文選題：苦豆子總堿　切入點：苦豆子灌注劑　出處：《甘肅農業(yè)大學》2015年碩士論文　論文類型：學位論文

【摘要】：本文以苦豆子總堿為研究對象,將其制成水溶性灌注劑,并建立該制劑的質量標準。選用單因素試驗及正交試驗并結合酸性染料比色法測定苦豆子總堿含量確立酸水提取法的最佳工藝路線。以苦豆子總堿對小鼠的急性試驗、苦豆子總堿對奶牛子宮內膜炎5種致病菌的抑菌試驗為制劑含藥量提供依據;分別采用高效液相色譜法(HPLC)和薄層色譜法(TLC)對苦豆子總堿灌注劑進行定量測定、定性鑒別,建立該制劑的質量標準。通過L9(34)正交試驗設計,最終確立最佳提取工藝條件為:中藥粉與酸水的體積比為1:20,浸泡時間為24h,0.6%的酸水,提取2次;通過酸性染料比色法測得總生物堿含量為7.08%。通過苦豆子總堿對小鼠的急性毒性試驗和苦豆子總堿對5種致病菌的抑菌效果,最終確定苦豆子總堿灌注劑有效藥量為120 mg/mL。通過TCL定性鑒別了處方中的槐定堿并用HPLC測定了處方中槐定堿的含量�？喽棺涌倝A的水溶液理化性質穩(wěn)定并具有良好的擴散能力,注入子宮后,既避免了局部藥物濃度過高引起的粘膜刺激,又起到清洗子宮內膜的效果�？喽棺涌倝A灌注劑制備工藝簡單、釋藥平緩、性質穩(wěn)定,且水制劑使用方便�？喽棺涌倝A是天然產物,不會產生耐藥性和藥物殘留,并且它具有良好的抑菌和抗炎效果;在抗生素濫用嚴重的社會現(xiàn)狀下,為苦豆子總堿灌注劑的研發(fā)提供了廣闊的研究潛力和應用前景。
[Abstract]:In this paper, the total alkaloids of Sophora alopecuroides were used as water soluble perfusers. To establish the quality standard of this preparation, single factor test and orthogonal test, combined with acid dye colorimetry to determine the content of total alkaloids of Sophora charantia L. the best technological route of acid water extraction method was established. The acute experiment of total alkaloids of Sophora alopecuroides on mice was carried out. The bacteriostatic test of total alkaloids of Sophora alopecuroides against 5 kinds of pathogenic bacteria in dairy cow endometritis provided the basis for the drug content of the preparation, the HPLC and TLC were used to determine the total alkaloid perfusion agent of Sophora alopecuroides quantitatively and qualitatively, respectively, and identified qualitatively. The quality standard of the preparation was established. The optimum extraction conditions were determined by orthogonal design: the volume ratio of traditional Chinese medicine powder to acid water was 1: 20, the soaking time was 24 hours, 0.6% acid water, 2 times extraction; The content of total alkaloids was 7.08 by acid dye colorimetry. The acute toxicity of total alkaloids of Sophora flavescens to mice and the bacteriostatic effect of total alkaloids of Sophora flavescens to 5 kinds of pathogenic bacteria were tested. Finally, the effective dosage of total alkaloid perfusion was determined to be 120mg / mL. Sophorine was identified qualitatively by TCL and the content of Sophorine was determined by HPLC. The physicochemical properties of the aqueous solution of total alkaloid were stable and had good diffusion ability. After injected into uterus, the mucosal irritation caused by local drug concentration was avoided and the endometrium was cleaned. The preparation process of total alkaloid perfusion agent of Sophora alopecuroides was simple, the drug release was smooth, and the nature was stable. Water preparation is convenient to use. Total alkaloids of Sophora alopecuroides are natural products, do not produce drug resistance and drug residues, and it has good bacteriostasis and anti-inflammatory effect. It provides broad research potential and application prospect for the research and development of total alkaloid perfusion agent of Sophora alopecuroides.
【學位授予單位】：甘肅農業(yè)大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：S853.73

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相關期刊論文 前1條

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本文編號：1651116