本文選題：全反式視黃酸　切入點(diǎn)：前脂肪細(xì)胞　出處：《吉林大學(xué)》2015年碩士論文　論文類型：學(xué)位論文

【摘要】：本研究旨在探討全反式視黃酸(all trans retinoic acid,ATRA)對(duì)3T3-L1前脂肪細(xì)胞增殖與分化的影響。培養(yǎng)3T3-L1前脂肪細(xì)胞，分別設(shè)置對(duì)照組（0mol/L）以及10-9、10-8、10-7、10-6和10-5mol/L濃度的ATRA處理組，分別在第0、2、4、6、7和8d通過MTS比色法檢測(cè)ATRA對(duì)3T3-L1前脂肪細(xì)胞增殖的影響；在誘導(dǎo)分化第12d，油紅O染色觀察3T3-L1前脂肪細(xì)胞分化的形態(tài)變化，油紅O染色提取法分析不同濃度ATRA對(duì)3T3-L1前脂肪細(xì)胞分化的影響，Real-timePCR技術(shù)檢測(cè)前脂肪細(xì)胞分化相關(guān)基因mRNA水平的表達(dá)。結(jié)果如下： （1）在3T3-L1前脂肪細(xì)胞生長(zhǎng)期間，與對(duì)照組（0mol/L）相比，10-9mol/L濃度的ATRA處理組不影響3T3-L1前脂肪細(xì)胞的增殖（P0.05）。10-8、10-7、10-6和10-5mol/L濃度的ATRA處理組隨著時(shí)間的增加，顯著抑制了細(xì)胞的增殖（P0.01）。其中，10-6和10-5mol/L濃度的ATRA處理組抑制3T3-L1前脂肪細(xì)胞增殖的效果最為明顯。 （2）與對(duì)照組（0mol/L）相比，，10-7、10-6和10-5mol/L濃度的ATRA抑制3T3-L1前脂肪細(xì)胞的分化(P0.01)，而10-9和10-8mol/L濃度的ATRA對(duì)3T3-L1前脂肪細(xì)胞分化沒有顯著影響(P0.05)。與10-7mol/L濃度的ATRA處理組相比，10-5mol/L濃度的ATRA對(duì)3T3-L1前脂肪細(xì)胞分化的抑制效果更為顯著(P0.05)。 （3）在誘導(dǎo)分化第12d，10-9、10-8、10-7、10-6和10-5mol/L濃度的ATRA處理組中PPARγ、C/EBPα、LXRα、FAS、SCD-1和Glut-4基因mRNA表達(dá)減少，與對(duì)照組（0mol/L）比較差異極顯著（P0.01）。 （4）在誘導(dǎo)分化第12d，10-7、10-6和10-5mol/L濃度的ATRA處理組中SREBP-1c基因mRNA表達(dá)上升，與對(duì)照組（0mol/L）比較差異極顯著（P0.01）。10-8mol/L濃度的ATRA處理組中ACCα基因mRNA表達(dá)上升，與對(duì)照組（0mol/L）比較差異顯著（P0.05）。10-5mol/L濃度的ATRA處理組中β-catenin基因mRNA表達(dá)上升，與對(duì)照組（0mol/L）比較差異極顯著（P0.01）。
[Abstract]:The purpose of this study was to investigate all trans retinoic acid (all trans retinoic acid, ATRA) and the influence on the differentiation of 3T3-L1 cells. The proliferation of cultured 3T3-L1 preadipocytes were set in the control group (0mol/L) and 10-9,10-8,10-7,10-6 and 10-5mol/L concentrations of ATRA groups, respectively in the 0,2,4,6,7 and 8D detected by ATRA MTS the color of 3T3-L1 preadipocyte proliferation; induced differentiation in 12D, to observe the morphological changes of 3T3-L1 preadipocyte differentiation oil red O staining, oil red O staining extraction analysis of different concentrations of ATRA on the differentiation of 3T3-L1 preadipocytes, Real-timePCR technology to detect the expression of preadipocyte differentiation related gene mRNA levels. Results the following:
(1) during 3T3-L1 preadipocyte cell growth, compared with the control group (0mol/L), ATRA treatment group 10-9mol/L concentration did not affect the proliferation of 3T3-L1 preadipocyte (P0.05).10-8,10-7,10-6 and 10-5mol/L concentrations of ATRA groups increased with time, significantly inhibited the cell proliferation (P0.01). Among them, the ATRA treatment group 10-6 and the concentration of 10-5mol/L inhibited the proliferation of 3T3-L1 preadipocytes has the most obvious effect.
(2) and control group (0mol/L), 10-7,10-6 and 10-5mol/L concentrations of ATRA inhibited the differentiation of 3T3-L1 preadipocyte (P0.01), and 10-9 10-8mol/L and concentration of ATRA on the differentiation of 3T3-L1 preadipocytes had no significant effect (P0.05). Compared with the ATRA group of 10-7mol/L concentration, the inhibitory effect of 10-5mol/L concentration of ATRA on the differentiation of 3T3-L1 preadipocytes was more significant (P0.05).
(3) in the ATRA treated group with the concentration of 12D, 10-9,10-8,10-7,10-6 and 10-5mol/L, the expression of PPAR, C/EBP, LXR, FAS, SCD-1 and Glut-4 decreased significantly compared with the control group (SCD-1).
(4) on the differentiation of 12D, SREBP-1c increased the expression of mRNA gene in ATRA treated 10-7,10-6 and 10-5mol/L concentration, and the control group (0mol/L) significantly increased ACC (P0.01) mRNA gene expression in ATRA treated.10-8mol/L concentration, and the control group (0mol/L) and there was significant difference (P0.05) increased beta -catenin gene expression of mRNA ATRA.10-5mol/L concentration in treatment group, and control group (0mol/L) significantly (P0.01).

【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：S816

【參考文獻(xiàn)】

相關(guān)期刊論文 前6條

1 田維熙，董妍，權(quán)暉，陳文峰;不同生長(zhǎng)期蛋雞的體脂水平和肝臟脂肪酸合成酶活性的關(guān)系[J];生物化學(xué)雜志;1996年02期

2 王爾松,金百祥;視黃酸受體及其信號(hào)轉(zhuǎn)導(dǎo)機(jī)制[J];國(guó)外醫(yī)學(xué)(生理、病理科學(xué)與臨床分冊(cè));1999年06期

3 熊文中,楊鳳,周安國(guó);豬重組生長(zhǎng)激素對(duì)不同雜交肥育豬脂肪代謝調(diào)控的研究[J];畜牧獸醫(yī)學(xué)報(bào);2001年01期

4 陳國(guó)柱,林亞秋,龐衛(wèi)軍,楊公社;全反式維甲酸對(duì)大鼠前體脂肪細(xì)胞增殖與分化的影響[J];畜牧獸醫(yī)學(xué)報(bào);2005年04期

5 朱曉海,何清濂,林子豪,趙耀忠,吳建明;常用維生素對(duì)人前脂肪細(xì)胞增殖和分化的作用[J];中華醫(yī)學(xué)美學(xué)美容雜志;2003年06期

6 裴星朝;周祿強(qiáng);葉超;李昂;;MTS和CCK-8法檢測(cè)番鴨胚胎成纖維細(xì)胞增殖的比較[J];中國(guó)家禽;2014年11期

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