發(fā)布時(shí)間：2018-03-21 21:15

  本文選題：肉雞　切入點(diǎn)：脂肪組織　出處：《畜牧獸醫(yī)學(xué)報(bào)》2017年09期 　論文類型：期刊論文

【摘要】：旨在準(zhǔn)確獲得肉雞腹脂雙向選擇系脂肪組織中的RNA編輯位點(diǎn)信息,進(jìn)而為脂肪相關(guān)研究提供更多的信息來(lái)源。本研究以東北農(nóng)業(yè)大學(xué)肉雞高、低腹脂雙向選擇品系第十九世代肉雞為試驗(yàn)材料,使用轉(zhuǎn)錄組測(cè)序(RNA-seq)和基因組測(cè)序數(shù)據(jù)識(shí)別肉雞腹部脂肪組織RNA編輯位點(diǎn)。通過(guò)生物信息學(xué)手段,設(shè)定嚴(yán)格的數(shù)據(jù)過(guò)濾與篩選策略,構(gòu)建RNA編輯位點(diǎn)候選集。結(jié)果表明,在候選集中,通過(guò)對(duì)RNA編輯位點(diǎn)的分類和注釋,在低脂系肉雞脂肪組織中發(fā)掘轉(zhuǎn)換型RNA編輯位點(diǎn)28個(gè),顛換型RNA編輯位點(diǎn)101個(gè),插入缺失型RNA編輯位點(diǎn)71個(gè);在高脂系肉雞脂肪組織中,發(fā)掘轉(zhuǎn)換型RNA編輯位點(diǎn)30個(gè),顛換型RNA編輯位點(diǎn)84個(gè),插入缺失型RNA編輯位點(diǎn)77個(gè)。絕大多數(shù)RNA編輯位點(diǎn)落在內(nèi)含子、基因間區(qū)域及基因上游區(qū)域(內(nèi)含子區(qū)41.91%,基因間區(qū)域33.08%,基因下游20.59%,基因上游2.94%和外顯子區(qū)1.47%)。試驗(yàn)驗(yàn)證了4個(gè)候選RNA編輯位點(diǎn),其中3個(gè)位點(diǎn)落在與脂肪形成有重要關(guān)系的ATP1B3、SLC6A6和FLNB基因區(qū)域。本研究鑒定和驗(yàn)證了存在于肉雞脂肪組織中的RNA編輯位點(diǎn),為進(jìn)一步研究RNA編輯影響脂肪組織生長(zhǎng)發(fā)育的分子機(jī)制奠定了基礎(chǔ)。
[Abstract]:The aim of this study was to obtain accurate information of RNA editing site in adipose tissue of bidirectional selection line of abdominal fat in broilers, and to provide more information sources for adipose related studies. Low abdominal fat bidirectional selection of the 19th generation broilers as experimental materials, using RNA-seq) and genomic sequencing data to identify RNA editing sites in abdominal adipose tissue of broilers. Strict data filtering and screening strategies were set up to construct the candidate set of RNA editing sites. The results showed that, by classifying and annotating RNA editing sites, 28 transformative RNA editing sites were found in adipose tissue of low-fat broilers. Among the adipose tissues of high-fat broilers, 30 were found to be transversive RNA editing sites, 84 were transversive RNA editing sites, and 101 were transversive RNA editing sites and 71 insertion deletion RNA editing sites, respectively. The majority of RNA editing sites fell in introns. Intergenic region and gene upstream region (intron region 41.91, intergenic region 33.08, downstream gene 20.5959, upstream 2.94% and exon 1.4775). Four candidate RNA editing sites were tested. Three of the loci were located in the region of ATP-1B3OSLC6A6 and FLNB gene, which were related to adipogenesis. The RNA editing site in adipose tissue of broiler was identified and verified. It provides a basis for further study on the molecular mechanism of RNA editing affecting the growth and development of adipose tissue.
【作者單位】： 東北農(nóng)業(yè)大學(xué)動(dòng)物科學(xué)技術(shù)學(xué)院農(nóng)業(yè)部雞遺傳育種重點(diǎn)實(shí)驗(yàn)室黑龍江省教育廳動(dòng)物遺傳育種與繁殖重點(diǎn)實(shí)驗(yàn)室;
【基金】：國(guó)家自然科學(xué)基金(31472088) 雞重要經(jīng)濟(jì)性狀的功能基因組學(xué)研究資助(863項(xiàng)目)(2013AA102501)
【分類號(hào)】：S831

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