本文選題：Duddingtonia　切入點(diǎn)：flagrans　出處：《西北農(nóng)林科技大學(xué)學(xué)報(bào)(自然科學(xué)版)》2017年09期 　論文類型：期刊論文

【摘要】：【目的】探究捕食線蟲性真菌Duddingtonia flagrans凍干制劑厚壁孢子的批量培養(yǎng)方法及其對線蟲幼蟲的毒殺劑量。【方法】通過玉米�；虼篼溋Ｅ囵B(yǎng)基的單步培養(yǎng)法,以及先在含有0.05%瓊脂粉的沙氏葡萄糖肉湯培養(yǎng)基中培養(yǎng)7d后繼而轉(zhuǎn)接到玉米粒或大麥粒培養(yǎng)基的雙步培養(yǎng)法,以培養(yǎng)3~5周后洗脫的每克固體培養(yǎng)基中的厚壁孢子數(shù)為依據(jù),對D.flagrans的最優(yōu)培養(yǎng)方法進(jìn)行篩選;然后使用最優(yōu)培養(yǎng)法培養(yǎng)D.flagrans厚壁孢子并將其凍干后用于體外殺線蟲幼蟲試驗(yàn),研究D.flagrans凍干制劑的殺線蟲幼蟲劑量�！窘Y(jié)果】培養(yǎng)基質(zhì)不同時(shí),D.flagrans的菌落顏色有一定差異,玉米粒培養(yǎng)基中的菌落顏色呈微黃色,而大麥粒培養(yǎng)基中的菌落呈白色;D.flagrans的適宜培養(yǎng)時(shí)間為3周。采用單步培養(yǎng)法時(shí),玉米粒培養(yǎng)基培養(yǎng)的厚壁孢子數(shù)量為2.4×10~5個(gè)/g,大麥粒培養(yǎng)基培養(yǎng)的厚壁孢子數(shù)量為3.2×10~5個(gè)/g;而采用雙步培養(yǎng)法時(shí),玉米粒培養(yǎng)基培養(yǎng)的厚壁孢子數(shù)量為3.0×10~5個(gè)/g,大麥粒培養(yǎng)基培養(yǎng)的厚壁孢子數(shù)量為3.5×10~5個(gè)/g。D.flagrans凍干制劑殺線蟲幼蟲劑量的閾值為每克糞便4×10~5個(gè)D.flagrans厚壁孢子,相應(yīng)對線蟲幼蟲的殺蟲率為95.2%�！窘Y(jié)論】采用雙步培養(yǎng)法培養(yǎng)厚壁孢子并將其制備成凍干制劑,在D.flagrans生物防治寄生性線蟲病方面有較好的應(yīng)用前景。
[Abstract]:[objective] to study the batch culture method of the freeze-dried preparation of nematodes, Duddingtonia flagrans, and its toxicity to nematode larvae. [methods] single step culture method was used to culture the nematode larvae in the medium of corn or wheat grains. And the two-step culture method was used to culture the medium containing 0.05% Agar powder for 7 days and then transferred to the medium of corn or wheat grains. The results were based on the number of thick parietal spores per gram of solid medium eluted after 35 weeks of culture. The optimum culture method of D. flagrans was screened, and the D. flagrans thick wall spores were cultured by the optimal culture method and then freeze-dried and used to kill nematode larvae in vitro. The dosage of D. flagrans freeze-dried preparation against nematode larvae was studied. [results] the colony color of D. flagrans was different in different culture medium, and the colony color of D. flagrans in cornkernel medium was yellowish. The optimum culture time of white D. flagrans in wheat medium was 3 weeks. The number of thick wall spores was 2.4 脳 10 ~ 5 / g in cornkernel medium and 3.2 脳 10 ~ 5 / g in wheat grain medium, while in the two-step culture method, the number of thick wall spores was 3.2 脳 10 ~ 5 / g. The number of thick wall spores was 3.0 脳 10 ~ 5 / g in cornkernel medium and 3.5 脳 10 ~ 5 / g 路D. flagrans in wheat medium. The threshold of larval dose of nematodes was 4 脳 10 ~ 5 D. flagrans per gram of feces, and the threshold value was 4 脳 10 ~ 5 D. flagrans thick wall spores per gram of feces. The killing rate of nematode larvae was 95.2%. [conclusion] using two-step culture method to culture thick wall spores and prepare them into freeze-dried preparation has a good application prospect in the field of D. flagrans biological control of parasitic nematode disease.
【作者單位】： 內(nèi)蒙古農(nóng)業(yè)大學(xué)獸醫(yī)學(xué)院農(nóng)業(yè)部動(dòng)物疾病臨床診療技術(shù)重點(diǎn)實(shí)驗(yàn)室;內(nèi)蒙古自治區(qū)農(nóng)牧業(yè)科學(xué)院獸醫(yī)研究所;
【基金】：國家公益性行業(yè)(農(nóng)業(yè))科研專項(xiàng)(201303037) 內(nèi)蒙古自然科學(xué)基金項(xiàng)目(2015MS0308) 家畜疫病病原生物學(xué)國家重點(diǎn)實(shí)驗(yàn)室開放基金項(xiàng)目(SKLVEB2015KFKT013)
【分類號(hào)】：S855.9
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本文編號(hào)：1626523