本文選題：肉雞　切入點(diǎn)：禽腺病毒　出處：《中國(guó)獸醫(yī)雜志》2017年01期 　論文類型：期刊論文

【摘要】：為了解山東省肉雞場(chǎng)禽腺病毒的感染流行特點(diǎn),為其科學(xué)防治提供依據(jù),通過臨床癥狀觀察、病毒分離鑒定、雞胚致病性試驗(yàn)、血凝試驗(yàn)、PCR檢測(cè)及克隆測(cè)序等方法進(jìn)行病原檢測(cè)分析。結(jié)果表明,發(fā)病肉雞主要表現(xiàn)為精神沉郁、采食減少等臨床癥狀。雞胚致病性試驗(yàn)可見死亡胚體呈全身出血、體型小、肝腫大現(xiàn)象。血凝試驗(yàn)顯示,分離株不具凝集雞、鴨、大鼠紅細(xì)胞的特性。對(duì)hexon基因進(jìn)行克隆測(cè)序分析顯示,LY9株與Ⅰ群禽腺病毒血清8型親緣性近,與參考株(58株)的核苷酸同源性為98.8%,氨基酸同源性為98.6%。其余8株分離株間的核苷酸同源性為91.3%~99.8%,氨基酸同源性為97.8%~100%;與Ⅰ群禽腺病毒血清4型參考株(HB1510株)屬同一進(jìn)化分支,核苷酸同源性為93.1%~100%,氨基酸同源性為98.9%~100%。結(jié)論:從山東省4家肉雞場(chǎng)分離到的9株病毒均為Ⅰ群禽腺病毒。
[Abstract]:In order to understand the epidemic characteristics of avian adenovirus infection in poultry farms in Shandong Province and provide scientific basis for its prevention and treatment, clinical symptom observation, virus isolation and identification, and chicken embryo pathogenicity test were carried out. The results showed that the main clinical symptoms of broiler disease were depression of mind and decrease of feed intake. The pathogenicity test of chicken embryo showed that the dead embryo body was bleeding all over the body and its size was small. Hepatomegaly. Hemagglutination test showed that the isolated strain did not possess the characteristics of agglutinated chicken, duck and rat red blood cells. Cloning and sequencing of hexon gene showed that LY9 strain was closely related to serotype 8 of group 鈪，

本文編號(hào)：1625622