本文選題：牛病毒性腹瀉病　切入點：ELISA　出處：《塔里木大學》2017年碩士論文　論文類型：學位論文

【摘要】：牛病毒性腹瀉病(Bovine viral diarrhea,BVD)是由牛病毒性腹瀉病毒(Bovine viral diarrhea virus,BVDV)感染引起的一種接觸性傳染病,根據(jù)感染毒株的不同可引起牛表現(xiàn)多種臨床癥狀,以粘膜潰瘍、糜爛及腹瀉為主要特征。近年來,BVD在新疆地區(qū)呈現(xiàn)蔓延趨勢,嚴重影響了新疆規(guī)模養(yǎng)牛業(yè)的持續(xù)健康發(fā)展,因此掌握本地區(qū)BVD的流行現(xiàn)況對防控該病具有重要意義。1.2015年新疆南疆13個規(guī)模奶牛場124份大罐奶樣進行BVDV抗體ELISA檢測,其中3個奶牛場抗體陽性檢出率為77.78%~82.35%,10個奶牛場抗體檢出率達到100%。2.2015、2016年度新疆南疆14個規(guī)模奶牛場1555份血清樣品進行BVDV抗體、抗原ELISA檢測,2015年A~N奶牛場BVDV抗體陽性率84.48%以上,抗原陽性率為0.78%;2016年A~N奶牛場BVDV抗體陽性率91.38%以上,抗原陽性率為0.25%。3.RT-PCR檢測共檢出9份陽性樣品。其中6株為BVDV Ic型,3株為BVDV I型未知亞型。表明新疆南疆規(guī)模化奶牛場存在BVDV Ic型和BVDV I型未知亞型。4.2016年9~10月3個規(guī)模奶牛場母牛、犢牛血清親子樣品各136份進行ELISA檢測,犢牛均為抗原陰性,僅有1頭母牛為抗原陽性并經(jīng)RT-PCR檢測,為BVDV I型未知亞型。本研究通過在新疆南疆部分規(guī)模奶牛場大罐奶樣BVD檢測、血清學流行病學調(diào)查、分子流行病學調(diào)查等,獲得新疆南疆部分規(guī)模奶牛場BVD流行病學資料及流行株基因型,為規(guī)模奶牛場BVD免疫及控制策略提供參考依據(jù)。
[Abstract]:Bovine viral diarrhea (BVD) is a contact infectious disease caused by bovine viral diarrhea virus (BVDV) infection. It can cause many clinical symptoms and mucosal ulcers according to the different strains of bovine viral diarrhea. Erosion and diarrhea are the main characteristics. In recent years, BVD has been spreading in Xinjiang, which has seriously affected the sustainable and healthy development of large-scale cattle farming in Xinjiang. Therefore, it is important to know the prevalence of BVD in this area. 1. In 2015, 124 large pot milk samples from 13 large dairy farms in Southern Xinjiang were detected for BVDV antibody ELISA. The positive rate of antibody in 3 dairy farms was 77.78 and 82.35, and the positive rate of 10 dairy farms reached 100.2.2015. 1555 serum samples from 14 scale dairy farms in Nanjiang of Xinjiang were tested for BVDV antibody and antigen ELISA. In 2015, the positive rate of BVDV antibody in An dairy farm was more than 84.48%. In 2016, the positive rate of BVDV antibody in Agnon dairy farm was more than 91.38%. The positive rate of antigen was 0.25. 3. 9 positive samples were detected by RT-PCR, of which 6 were BVDV Ic type and 3 were unknown BVDV I subtypes, which indicated that there were unknown BVDV I and BVDV I subtypes in large-scale dairy farms in southern Xinjiang. 4. From 9 to October 2016, 3 dairy cows of large scale were identified. The serum samples were tested for ELISA, and only 1 cow was antigen-positive and tested by RT-PCR for unknown BVDV I subtype. In this study, BVD was detected in large pot milk samples of part scale dairy farms in southern Xinjiang. Serological epidemiological investigation and molecular epidemiological investigation were conducted to obtain BVD epidemiological data and genotypes of epidemic strains in some large-scale dairy farms in southern Xinjiang. The results provided a reference basis for BVD immunization and control strategies in large-scale dairy farms.
【學位授予單位】：塔里木大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：S858.23

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