本文選題：玉米秸稈　切入點：稻草　出處：《動物營養(yǎng)學報》2017年02期 　論文類型：期刊論文

【摘要】：本試驗旨在應用體外產氣法研究玉米秸稈、稻草、玉米秸稈青貯與精料間的組合效應。采用單因素試驗設計,進行3次組合篩選試驗:首先進行玉米秸稈與稻草組合試驗,篩選出最優(yōu)玉米秸稈和稻草組合(玉米秸稈-稻草)比例;再進行玉米秸稈-稻草與玉米秸稈青貯組合試驗,篩選出最優(yōu)玉米秸稈-稻草和玉米秸稈青貯組合(玉米秸稈-稻草-玉米秸稈青貯)比例;最后進行玉米秸稈-稻草-玉米秸稈青貯與精料組合試驗,篩選出最優(yōu)玉米秸稈-稻草-玉米秸稈青貯與精料組合比例。各組合均分別以100.0∶0、80.0∶20.0、60.0∶40.0、50.0∶50.0、40.0∶60.0、20.0∶80.0、0∶100.0進行體外發(fā)酵試驗,每個組合設3個重復。利用體外產氣法分析不同飼料組合對48 h產氣量、干物質消失率(DMD)、p H及微生物蛋白(MCP)、氨態(tài)氮(NH3-N)、揮發(fā)性脂肪酸(VFA)濃度的影響。計算各組合的單項組合效應指數(SFAEI)和多項組合效應指數(MFAEI)。結果表明:1)各飼料以不同比例組合對產氣量均有顯著或極顯著影響(P0.05或P0.01),玉米秸稈∶稻草、玉米秸稈-稻草∶玉米秸稈青貯、玉米秸稈-稻草-玉米秸稈青貯∶精料產氣量的SFAEI分別在60.0∶40.0、40.0∶60.0、20.0∶80.0時達最大值;2)各飼料以不同比例組合對DMD也存在顯著或極顯著影響(P0.05或P0.01),玉米秸稈∶稻草、玉米秸稈-稻草∶玉米秸稈青貯、玉米秸稈-稻草-玉米秸稈青貯∶精料DMD的SFAEI分別在50.0∶50.0、40.0∶60.0、20.0∶80.0時達最大值;3)不同玉米秸稈-稻草-稻草∶精料對p H有極顯著影響(P0.01);4)各飼料以不同比例組合對MCP濃度也存在顯著或極顯著影響(P0.05或P0.01),玉米秸稈∶稻草、玉米秸稈-玉米秸稈青貯∶玉米秸稈青貯、玉米秸稈-稻草-玉米秸稈青貯∶精料產氣量的SFAEI分別在80.0∶20.0、40.0∶60.0、20.0∶80.0時達最大值;5)各飼料以不同比例組合對NH3-N濃度也存在顯著或極顯著影響(P0.05或P0.01),范圍在20.20~31.59 mg/d L;6)玉米秸稈與稻草組合的乙酸/丙酸和玉米秸稈-稻草-玉米秸稈青貯與精料組合丁酸濃度在各比例間差異不顯著(P0.05),各飼料以不同比例組合對其余VFA和TVFA濃度也存在顯著或極顯著影響(P0.05或P0.01)。以MFAEI進行評定各飼料組合的最優(yōu)比例如下:玉米秸稈與稻草為60.0∶40.0;玉米秸稈、稻草與玉米秸稈青貯為24.0∶16.0∶60.0;玉米秸稈、稻草、玉米秸稈青貯與精料為9.6∶6.4∶24.0∶60.0。
[Abstract]:The purpose of this study was to study the combination effect of corn straw, straw, corn straw silage and concentrate by gas production in vitro. The optimum ratio of corn straw and straw (corn straw straw straw) was selected, and then the combination test of corn straw straw and corn straw silage was carried out. The optimal ratio of corn straw straw and corn straw silage (corn straw straw straw silage) was selected. Finally, the combination experiment of corn straw straw straw and corn straw silage and concentrate was carried out. The optimum proportion of corn straw straw straw silage and corn straw silage and concentrate was screened out. The in vitro fermentation experiment was carried out with 100.010: 0 0: 80.020.0% 60.040: 50.050.020: 40.060.020. 0: 80.00: 100.0. Each combination had 3 repetitions. The gas production of different feed combinations for 48 h was analyzed by in vitro gas production method. The effects of dry matter disappearance rate and microbial protein (MCPP), ammonia nitrogen (NH _ 3-N), volatile fatty acid (VFA) concentrations on the concentration of dry matter were calculated. The single combination effect index (SFAEI) and the multiple combination effect index (MFAEI) of each combination were calculated. The results showed that each feed was fed in different proportions. The combination had significant or extremely significant effects on gas production, P 0.05 or P 0.01, corn straw: straw, Corn straw-straw: corn straw silage, Corn straw, straw and corn straw silage: SFAEI of concentrate gas production reached the maximum value at 60.0: 40.010: 60.010: 20.080.0 respectively. Each feed combination had significant or extremely significant effect on DMD (P0.05 or P0.01g). Corn straw: straw: straw: straw. Corn straw-straw: corn straw silage, Corn Straw-Straw-Corn Straw silage: concentrate DMD SFAEI reached maximum value at 50.0: 50.0: 60.00: 20.0: 80.0) different Corn Straw-Straw-Straw: concentrate had a significant effect on pH (P0.01P0. 01%) different feed combinations also affected MCP concentration in different proportion. There were significant or extremely significant effects on P 0.05 or P 0.01, corn straw: straw, Corn straw silage: corn straw silage, Corn straw, straw and corn straw silage: SFAEI of concentrate gas production reached the maximum value at 80: 20.0 0: 60: 60: 20.0: 80.0) different feed combinations also had significant or extremely significant effects on NH3-N concentration (P0.05 or P0.01), and the range was 20.200.59 mg/d / L 6). Butyric acid concentration of acetic acid / propionic acid and corn straw straw silage and concentrate had no significant difference among different proportions, and the other concentrations of VFA and TVFA were also significantly affected by different feed combinations. The results showed that the optimum ratio of corn straw and straw was 60.040: 40.00.The optimum ratio of corn straw and straw was 60.040%, and the ratio of corn straw to straw was 60.040%, and the ratio of corn straw to straw was 60.040%. Straw and corn straw silage were 24.0: 16.0: 60.0.The corn straw, straw, corn straw silage and concentrate were 9.66.4: 24.0: 60.0.
【作者單位】： 河北農業(yè)大學動物科技學院;河北農業(yè)大學動物醫(yī)學院;
【基金】：公益性行業(yè)(農業(yè))科研專項經費(201503134) 河北省科技計劃項目(16226604D) 國家現代農業(yè)產業(yè)技術體系建設專項資金
【分類號】：S816.53

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