發(fā)布時間：2018-03-14 17:44

  本文選題：抗菌肽　切入點：海蘭褐蛋雞　出處：《吉林大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：本試驗隨機選取360只產(chǎn)蛋率接近、健康的383日齡海蘭褐蛋雞作為試驗動物。隨機分為2組,每個組內(nèi)設(shè)3個重復(fù)組,每個重復(fù)60只蛋雞。對照組蛋雞的日糧為基礎(chǔ)日糧,試驗組在基礎(chǔ)日糧中添加0.01L/kg抗菌肽。預(yù)飼期7d,試驗期35d。本試驗通過比較兩組蛋雞的生產(chǎn)性能、血清生化指標(biāo)、雞蛋營養(yǎng)成分,分析抗菌肽對產(chǎn)蛋后期蛋雞生產(chǎn)性能及雞蛋中膽固醇等含量的影響。在此基礎(chǔ)上利用RT-PCR技術(shù)檢測蛋雞肝臟中HMGCR m RNA和Apo-Ⅱ m RNA表達(dá)量的變化。本試驗?zāi)康氖翘岣叩半u產(chǎn)蛋后期的生產(chǎn)性能,提高蛋雞生產(chǎn)的經(jīng)濟效益,為生產(chǎn)低膽固醇雞蛋提供理論基礎(chǔ)和科學(xué)依據(jù)。試驗結(jié)果如下:1.生產(chǎn)性能:試驗組蛋雞產(chǎn)蛋率比對照組提高7.07%,且差異顯著(P0.05);與對照組相比,試驗組破蛋率降低12.19%,平均蛋重降低0.96%,料蛋比降低0.83%,但差異均不顯著(P0.05)。2.血清生化指標(biāo):試驗組蛋雞血清總蛋白比對照組顯著降低7.2%(P0.05),試驗組低密度脂蛋白膽固醇比對照組降低12.6%,且差異顯著(P0.05);試驗組蛋雞血清白蛋白和球蛋白水平相比對照組分別降低0.82%和9.48%,總膽固醇和甘油三酯分別升高4.39%和20.16%,血糖降低4.09%,差異均不顯著(P0.05)。3.雞蛋營養(yǎng)成分:與對照組比較,試驗組雞蛋中膽固醇含量較對照組顯著降低18.61%(P0.05);雞蛋中各項氨基酸含量較對照組均有不同程度的提高,總氨基酸比對照組提高6.79%,但差異均不顯著(P0.05)。試驗組雞蛋中P、Mg、Zn、Se含量較對照組均有提高,Ca的含量有所降低,但差異均不顯著(P0.05)。4.HMGCR和Apo-Ⅱ基因表達(dá)水平:試驗組的HMGCR m RNA表達(dá)量顯著降低(P0.05);Apo-Ⅱ m RNA表達(dá)量有所降低,但并無顯著變化(P0.05)。結(jié)論:1.抗菌肽使蛋雞產(chǎn)蛋率顯著提高了7.07%,一定程度上提高了海蘭褐蛋雞產(chǎn)蛋后期的生產(chǎn)性能。2.抗菌肽顯著降低了海蘭褐蛋雞血清的總蛋白水平及低密度脂蛋白膽固醇水平,促進(jìn)了膽固醇的轉(zhuǎn)運。3.抗菌肽使雞蛋中膽固醇含量顯著降低了18.61%,改善了雞蛋的營養(yǎng)成分。4.抗菌肽顯著降低了海蘭褐蛋雞肝臟HMGCR m RNA的表達(dá)量,抑制了蛋雞膽固醇的內(nèi)源性合成。。
[Abstract]:360 Hailan brown laying hens of 383 days old were randomly selected as experimental animals. Each group was divided into 2 groups, each group was divided into 3 repeats groups with 60 layers per replica. The diet of the control group was used as the basal diet. In the trial group, 0.01L / kg antimicrobial peptide was added to the basal diet. The prefeeding period was 7 days and the trial period was 35 days. The production performance, serum biochemical index and egg nutrition were compared between the two groups. The effect of antimicrobial peptide on the performance and cholesterol content of laying hens in late laying stage was analyzed. The expression of HMGCR m RNA and Apo- 鈪，

本文編號：1612272