本文選題：黑山羊　切入點(diǎn)：捻轉(zhuǎn)血矛線蟲　出處：《湖南農(nóng)業(yè)大學(xué)》2015年碩士論文　論文類型：學(xué)位論文

【摘要】：本研究在對瀏陽羊捻轉(zhuǎn)血矛線蟲感染情況的基礎(chǔ)上,對捻轉(zhuǎn)血矛線蟲進(jìn)行了形態(tài)學(xué)和分子生物鑒定,旨在為防治羊線蟲病提供科學(xué)依據(jù)。本研究的第一部分是采用剖檢方法調(diào)查瀏陽東南西北以及城區(qū)的黑山羊捻轉(zhuǎn)血矛線蟲感染情況,結(jié)果表明東南西北城區(qū)感染率分別為：75.00%、66.67%、57.14%、40.00%和35.71%；其中東區(qū)黑山羊捻轉(zhuǎn)血矛線蟲感染率最高為75.00%,城區(qū)采集的黑山羊捻轉(zhuǎn)血矛線蟲感染率最低為35.71%。本研究的第二部分對瀏陽市黑山羊捻轉(zhuǎn)血矛線蟲線粒體cox1, cox3, cytb基因的序列分析,結(jié)果發(fā)現(xiàn)本研究中的線粒體cox1, cox3, cytb基因片段,coxl基因A+T堿基含量為64.14%-65.97%,G+C堿基為34.03%-35.87%；cox3基因A+T堿基含量為69.58%-70.87%,G+C堿基為29.13%-30.42%；cytb基因的A+T堿基含量為72.30%-74.37%,G+C堿基為25.69%-27.71%。利用Mega 4.1軟件對7株捻轉(zhuǎn)血矛線蟲進(jìn)行序列分析,結(jié)果顯示七株捻轉(zhuǎn)血矛線蟲voxl基因序列種內(nèi)變異率0.9--4.8%,種間9.4-16%；cox3基因序列種內(nèi)變異率1.2--6.9%；cytb基因序列種內(nèi)變異率1.8—8.2%。
[Abstract]:In this study, morphological and molecular biological identification were carried out on the basis of the infection of the blood spear nematode in Liuyang sheep. In order to provide scientific basis for the prevention and control of sheep nematode disease, the first part of this study was to investigate the infection of blood nematode twirling in black goats in Liuyang, east, south, northwest and urban areas by using dissecting method. The results showed that the infection rate was 40.00% and 35.71in the east east black goat and the black goat in the east, and the lowest infection rate was 35.71.The second part of this study was about the second part of this study, and the lowest infection rate of the black goat was 35.71.The second part of this study was about the second part of this study. The results showed that the infection rate was 75.00% in the east and 35.71l in the urban area respectively, and the highest infection rate was 75.00 in the eastern part of the black goat. Sequence analysis of mitochondrial cox1, cox3 and cytb genes of Haemonema japonicus in Yangshi Black Goat. The results showed that the content of mitochondrial cox1, cox3, cytb gene A T base was 64.14- 65.97g C base and 34.03-35.87Cox3 gene A T base content was 69.58-70.87G C base was 29.1313-30.42cyt b gene A T base content was 72.30-74.37G C base was 25.-27.71.The content of Mega 4.1 was 25.-27.71. The software was used to analyze the sequence of 7 strains of blood nematode twisting. The results showed that the intraspecific variation rate of voxl gene sequence was 0.9-4.8, and that of 9.4-16and cox3 gene sequence was 1.2--6.9. the variation rate of voxl gene sequence was 1.8-8.2%.
【學(xué)位授予單位】：湖南農(nóng)業(yè)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：S858.27

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