本文選題：CpG　切入點：ODN　出處：《中國獸藥雜志》2017年05期 　論文類型：期刊論文

【摘要】：為獲得對水貂具有免疫刺激活性的CpG ODN序列,設(shè)計合成了45種含不同CpG基序的DNA序列,應(yīng)用MTS比色法測定合成CpG ODNs刺激水貂PBMC增殖的能力,結(jié)果有11條CpG ODN對水貂PBMC有刺激活性(SI2);應(yīng)用SI值大于5的6種CpG ODN分別與水貂偽狂犬滅活疫苗聯(lián)合免疫水貂,免疫后經(jīng)水貂血清抗偽狂犬中和抗體效價測定和水貂PBMC非特異性增殖效應(yīng)檢測,結(jié)果有3個CpG ODN序列對水貂具有較好免疫增強作用,分別是CpG-21(ATCGATTTGTCGTTATCGAT)、CpG-23(ATCGATGAACGTTAACGTTTC)和CpG-24(AACGTTCATCGATATCGATGT)。本研究獲得3條對水貂具有較好免疫刺激活性的CpG ODN序列,可為水貂新型CpG佐劑的研究提供參考。
[Abstract]:In order to obtain CpG ODN sequences with immunostimulatory activity in minks, 45 DNA sequences with different CpG motifs were designed and synthesized. The ability of synthetic CpG ODNs to stimulate PBMC proliferation of mink was determined by MTS colorimetry. Results there were 11 CpG ODN stimulative activity to mink PBMC, six kinds of CpG ODN with SI value more than 5 were used to immunize mink with mink pseudorabies inactivated vaccine, respectively. After immunization, the titer of anti-pseudorabies neutralizing antibody in mink serum and the nonspecific proliferative effect of mink PBMC were determined. The results showed that three CpG ODN sequences had a better immune enhancement effect on minks. They are CpG-21, ATCGATTTGTCGTTATCGATT, CpG-23, ATCGATGAACGTTAACGTTTCand CpG-24, AACGTTCATCGATATCGATTTTTT.The three CpG ODN sequences with good immunostimulatory activity in minks were obtained in this study, which may provide a reference for the study of novel CpG adjuvants in minks.
【作者單位】： 吉林農(nóng)業(yè)大學(xué)中藥材學(xué)院;
【基金】：吉林省科技發(fā)展計劃項目(20140307008NY,20140412009XH)
【分類號】：S858.92

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本文編號：1610650