本文選題：麻葉蕁麻　切入點：狹葉蕁麻　出處：《新疆農(nóng)業(yè)大學》2015年碩士論文　論文類型：學位論文

【摘要】：新疆蕁麻資源豐富,尤其以麻葉蕁麻(Urtica Cannabina,UC)和狹葉蕁麻(Urtica Angustifolia,UA)分布最廣。為了探究蕁麻對平滑肌運動的影響,本研究比較了UC和UA對鼠子宮和小腸運動的影響,并運用受體或離子通道阻斷策略初步探討其可能機制,以期為蕁麻用于治療胃腸或子宮運動異常疾病提供參考。首先,用生物信號處理系統(tǒng)檢測了濃度遞增的UC和UA醇提液對未孕大鼠離體子宮平滑肌運動的影響及機制。結(jié)果顯示,UC濃度遞增到4.0%時較用藥前顯著增加子宮收縮頻率(P0.05),遞增到4.0%~8.0%時較用藥前顯著降低收縮幅度,但對子宮運動張力影響不明顯;加入0.5%UA后子宮的收縮運動受到抑制,但濃度遞增到2.0%以后,子宮收縮張力增加,頻率加快,當濃度遞增到8.0%時子宮出現(xiàn)僵直并且張力逐漸下降,直到完全抑制。UC(2.0%)和M-受體阻斷劑阿托品(6.2×10-6 mol/L)聯(lián)合用藥,較用藥前使子宮收縮頻率顯著加快,收縮張力增加(P0.05),但較UC單獨用藥顯著減低收縮張力(P0.05)。其次,探究了濃度遞增的UC和UA醇提液對禁食大鼠離體小腸平滑肌運動的影響及可能作用的機制。結(jié)果顯示,當UC濃度遞增到2.0%時能引起小腸收縮頻率、收縮張力和收縮幅度增加,在UC濃度遞增到4.0%~8.0%時,收縮頻率和收縮幅度較用藥前顯著降低(P0.05)。加入0.5%UA小腸運動表現(xiàn)短暫性僵直趨勢,之后隨著濃度遞增表現(xiàn)為收縮張力和幅度增加,但當濃度遞增到2.0%~4.0%時開始出現(xiàn)抑制作用。鈣離子通道阻滯劑維拉帕米(2×10-7 mol/L)和UC(2.0%)聯(lián)合用藥較用藥前以及UC單獨用藥顯著抑制小腸的收縮頻率、收縮幅度和收縮張力(P0.05)。將UC分別同酚妥拉明、雷尼替丁、苯海拉明和吲哚美辛聯(lián)合用藥,對小腸運動影響同UC單獨用藥沒有差異(P0.05)。最后,測定了10.0%、2.0%和0.4%UC對禁食小鼠在體胃排空率和腸推進率的影響。結(jié)果顯示,各濃度UC、生理鹽水空白對照和氨甲酰甲膽堿陽性對照均使小鼠胃排空率達95%以上,并且各組之間差異不明顯(P0.05)。在對腸推進率方面,各濃度UC和氨甲酰甲膽堿較生理鹽水組顯著增加小腸推進率(P0.05),阿托品單獨用藥較同0.4%UC聯(lián)合用藥顯著減小小腸推進率(P0.05),且這兩組均較生理鹽水組減小小腸推進率(P0.05)。本研究結(jié)果表明,UC劑量依賴性促進大鼠子宮和腸平滑肌運動,而UA在不同濃度時對離體子宮和腸平滑肌運動表現(xiàn)出興奮或抑制的雙向效應。UC對胃腸和子宮運動的興奮作用可能同激動M-受體和鈣離子通道有關,蕁麻有望用于治療胃腸或子宮動力不足相關疾病。
[Abstract]:Urtica Cannabina (UCC) and Urtica Angustifolia UAA (Urtica Angustifolia) are the most abundant resources in Urtica angustifolia. In order to investigate the effect of Urtica Angustifolia (UAA) on smooth muscle movement, the effects of UC and UA on the movement of uterus and small intestine in mice were compared. In order to provide reference for the treatment of gastrointestinal or uterine dysmotility, the receptor or ion channel blocking strategy was used to explore the possible mechanism of urticaria. The effects of increasing concentration of UC and UA on the movement of uterine smooth muscle in vitro of non-pregnant rats were investigated by using biological signal processing system. The results showed that the frequency of uterine contraction was significantly increased when the concentration of UCU increased to 4.0. The amplitude of contraction was significantly decreased when P0. 05% was increased to 4. 0 ~ 8. 0. The contractile movement of uterus was inhibited after adding 0.5 UA, but after increasing to 2.0%, the uterine contraction tension increased and the frequency increased. When the concentration increased to 8.0, the uterus became stiff and the tension gradually decreased. Up to complete inhibition (UCN 2.0) and M- receptor blocker atropine (6.2 脳 10 -6 mol / L), the uterine contraction frequency was significantly increased and the contraction tension was increased (P 0.05), but the contraction tension was significantly decreased than that of UC alone. The effects of increasing UC and UA concentrations on the movement of isolated small intestinal smooth muscle of fasting rats and the possible mechanism were investigated. The results showed that the increase of UC concentration to 2.0 could induce the contraction frequency, contraction tension and contraction amplitude of small intestine. When the concentration of UC increased to 4.0 ~ 8.0, the contractile frequency and amplitude were significantly lower than those before the treatment. The intestinal motion showed a transient rigidity trend after adding 0.5UA, and then the contractile tension and amplitude increased with the increase of the concentration. But the inhibition began to appear when the concentration increased to 2.0-4.0. The calcium channel blocker verapamil (2 脳 10-7 mol / L) and UC2.0) significantly inhibited the contraction frequency of small intestine compared with those before treatment and UC alone. Contractile amplitude and contraction tension (P0.05). There was no difference in the effect of UCU with Phenoto#internal_person0#, ranitidine, phenhydramine and indomethacin on the small intestine motility compared with UC alone (P0.05). The effects of 2.0% and 0.4UC on gastric emptying rate and intestinal propulsive rate in fasting mice were determined. The results showed that the gastric emptying rate of mice was more than 95% in each concentration of UC, normal saline blank control and carbamylcholine positive control. And there was no significant difference among the groups (P 0.05). Compared with normal saline group, all concentrations of UC and carbamylcholine significantly increased the small intestinal propulsive rate (P0.05), and atropine alone significantly decreased the intestinal propulsive rate (P0.05N) compared with the same 0.4 UC group, and both groups decreased the intestinal propulsive rate (P0.05N) compared with the normal saline group (P 0.05). The results showed that UCU promoted the smooth muscle movement of uterus and intestine in a dose-dependent manner. At different concentrations, UA showed a bidirectional effect of stimulation or inhibition on the smooth muscle movement of isolated uterus and intestine. The stimulation effect of UC on gastrointestinal and uterine motility may be related to the activation of M- receptor and calcium channel. Urticaria is expected to be used to treat gastrointestinal or uterine dysmotility related diseases.
【學位授予單位】：新疆農(nóng)業(yè)大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：S853.7

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