本文選題：長(zhǎng)江三角洲白山羊　切入點(diǎn)：優(yōu)質(zhì)筆料毛　出處：《揚(yáng)州大學(xué)》2015年碩士論文　論文類(lèi)型：學(xué)位論文

【摘要】：長(zhǎng)江三角洲白山羊是國(guó)內(nèi)外唯一以生產(chǎn)優(yōu)質(zhì)筆料毛為特征的肉、皮、毛兼用型山羊品種,其所生產(chǎn)的優(yōu)質(zhì)筆料毛是制作高檔毛筆的獨(dú)特原料。長(zhǎng)江三角洲白山羊是我國(guó)珍貴的不可替代的種質(zhì)資源,由于其獨(dú)特的產(chǎn)毛性能,又有筆料毛山羊之稱(chēng)。目前,對(duì)長(zhǎng)江三角洲白山羊優(yōu)質(zhì)筆料毛性狀的研究主要集中在宏觀領(lǐng)域,在分子水平方面的研究尚未深入開(kāi)展,本研究是在本課題組前期相關(guān)分子機(jī)制研究的基礎(chǔ)上,主要是通過(guò)RNA-Seq技術(shù)對(duì)長(zhǎng)江三角洲白山羊優(yōu)質(zhì)筆料毛性狀做進(jìn)一步研究,以深入探討該性狀形成的分子機(jī)理。本試驗(yàn)通過(guò)對(duì)長(zhǎng)江三角洲白山羊優(yōu)質(zhì)筆料毛型和非優(yōu)質(zhì)筆料毛型山羊皮膚轉(zhuǎn)錄組進(jìn)行高通量測(cè)序,旨在篩選可能影響長(zhǎng)江三角洲白山羊優(yōu)質(zhì)筆料毛性狀的重要基因及相關(guān)通路,并通過(guò)PCR-SSCP技術(shù)對(duì)相關(guān)基因做多態(tài)性分析。同時(shí)利用冷凍切片技術(shù)來(lái)比較優(yōu)質(zhì)筆料毛型與非優(yōu)質(zhì)筆料毛型個(gè)體頸脊部及背部皮膚毛囊的結(jié)構(gòu),旨在尋找兩種性狀毛囊結(jié)構(gòu)的區(qū)別。本研究得到以下結(jié)論：(1)優(yōu)質(zhì)筆料毛型和非優(yōu)質(zhì)筆料毛型山羊共有差異表達(dá)基因295個(gè)；以非優(yōu)質(zhì)筆料毛型山羊?yàn)閷?duì)照,在優(yōu)質(zhì)筆料毛型山羊皮膚組織中,表達(dá)上調(diào)基因132個(gè),表達(dá)下調(diào)基因62個(gè),特異表達(dá)基因有101個(gè)。(2)根據(jù)差異表達(dá)基因GO顯著性富集分析、代謝通路顯著性富集分析和表達(dá)量差異倍數(shù),篩選出MAPSK1基因和MAPK信號(hào)通路可能在長(zhǎng)江三角洲白山羊優(yōu)質(zhì)筆料毛性狀形成過(guò)程中起重要作用。(3) NCBI山山羊MAP3K1基因的十個(gè)SNP錯(cuò)義位點(diǎn)在優(yōu)質(zhì)筆料毛型和非優(yōu)質(zhì)筆料毛型山羊個(gè)體中未發(fā)現(xiàn)。(4)優(yōu)質(zhì)筆料毛型個(gè)體與非優(yōu)質(zhì)筆料毛型個(gè)體頸脊部及背部皮膚毛囊結(jié)構(gòu)無(wú)差異,但兩種筆料毛型個(gè)體頸脊部初級(jí)毛囊密度顯著低于優(yōu)質(zhì)型個(gè)體背部初級(jí)毛囊的密度。本研究獲得了優(yōu)質(zhì)筆料毛型和非優(yōu)質(zhì)筆料毛型山羊所有Unigene的注釋信息,建立了差異表達(dá)基因庫(kù),獲得了所有差異表達(dá)基因所屬代謝通路的信息,為進(jìn)一步研究長(zhǎng)江三角洲白山羊優(yōu)質(zhì)筆料毛性狀的形成機(jī)理提供理論依據(jù)。
[Abstract]:The Yangtze River Delta White Goat is the only breed of meat, skin and wool that is characterized by the production of high quality pen hair at home and abroad. The high quality brush hair produced by it is a unique raw material for making high-grade brush. The Yangtze River Delta white goat is a precious and irreplaceable germplasm resource in China, and it is also known as the pen hair goat because of its unique wool production performance. The research on the quality of white goats' hair traits in the Yangtze River Delta is mainly focused on the macroscopic field, but the study on molecular level has not been carried out in depth. This study is based on the previous study of molecular mechanism in our group. The main purpose of this study was to further study the quality of pen and wool characters of white goats in the Yangtze River Delta by RNA-Seq technique. In order to explore the molecular mechanism of the formation of this trait, high throughput sequencing was carried out on the skin transcriptome of high quality pen hair type and non quality pen hair type of Yangtze River Delta white goat. The aim of this study was to screen important genes and related pathways that might affect the quality of hair traits of white goats in the Yangtze River Delta. The polymorphism of related genes was analyzed by PCR-SSCP technique. The structure of cervical spine and back skin hair follicles of individuals with high quality pen hair and non-high quality pen hair were compared by using frozen section technique at the same time. The aim of this study was to find out the difference of hair follicle structure between the two traits. In this study, we got the following conclusion: 1) there are 295 differentially expressed genes between fine and non-high quality pen hair goats. In the skin tissues of high quality pencil-hair goats, 132 up-regulated genes, 62 down-regulated genes and 101 specific expression genes were detected. Significant enrichment of metabolic pathways and multiple differences in expression levels, Screening of MAPSK1 gene and MAPK signaling pathway may play an important role in the formation of high quality pen hair traits of Yangtze River Delta white goats.) Ten SNP missense sites of MAP3K1 gene of NCBI mountain goat are found to be in high quality pen hair type and non-high quality pen hair type. There was no difference in the structure of hair follicles in the cervical spine and back between the individuals with high quality pen hair and those with non-high quality pen hair, and there was no significant difference in the structure of hair follicles in the neck and back between the individuals with high quality pen and hair. However, the density of the primary hair follicles in the cervical spine of the two types of individuals was significantly lower than that of the primary hair follicles in the back of the high quality individuals. In this study, the annotated information of all Unigene of the high quality pen hair goats and the non-high quality wool goats were obtained. The differential expression gene bank was established, and the information of metabolic pathway of all differentially expressed genes was obtained, which provides a theoretical basis for the further study of the formation mechanism of high quality hair traits of white goats in the Yangtze River Delta.
【學(xué)位授予單位】：揚(yáng)州大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類(lèi)號(hào)】：S827

【相似文獻(xiàn)】

相關(guān)期刊論文 前4條

1 殷亞娟;曹廣力;黃茉莉;薛仁宇;貢成良;;基于RNA-Seq分析幼蟲(chóng)5齡期至化蛹初期家蠶卵巢發(fā)育中的細(xì)胞進(jìn)程相關(guān)基因[J];蠶業(yè)科學(xué);2013年06期

2 江南;譚曉風(fēng);張琳;;基于RNA-Seq的油茶種子油脂合成過(guò)程磷脂酰肌醇信號(hào)系統(tǒng)的基因分析[J];食品與生物技術(shù)學(xué)報(bào);2014年06期

3 蘭道亮;熊顯榮;位艷麗;徐通;鐘金城;字向東;王永;李鍵;;基于RNA-Seq高通量測(cè)序技術(shù)的牦牛卵巢轉(zhuǎn)錄組研究:進(jìn)一步完善牦�；蚪Y(jié)構(gòu)及挖掘與繁殖相關(guān)新基因[J];中國(guó)科學(xué):生命科學(xué);2014年03期

4 ;[J];;年期

相關(guān)會(huì)議論文 前3條

1 趙西西;王成成;陳國(guó)華;林劍青;賀竹梅;;RNA-Seq揭示黃曲霉胞內(nèi)氧化還原反應(yīng)與黃曲霉毒素合成的關(guān)系[A];中國(guó)遺傳學(xué)會(huì)第九次全國(guó)會(huì)員代表大會(huì)暨學(xué)術(shù)研討會(huì)論文摘要匯編（2009-2013）[C];2013年

2 張玉剛;祝軍;梁美霞;戴洪義;;利用RNA-Seq分析柱型和普通型蘋(píng)果轉(zhuǎn)錄水平差異[A];中國(guó)園藝學(xué)會(huì)2011年學(xué)術(shù)年會(huì)論文摘要集[C];2011年

3 于安民;楊錦芬;;基于RNA-seq的陽(yáng)春砂果實(shí)可溶性糖及萜類(lèi)代謝的基因表達(dá)譜分析[A];生態(tài)文明建設(shè)中的植物學(xué)：現(xiàn)在與未來(lái)——中國(guó)植物學(xué)會(huì)第十五屆會(huì)員代表大會(huì)暨八十周年學(xué)術(shù)年會(huì)論文集——第4分會(huì)場(chǎng)：資源植物學(xué)[C];2013年

相關(guān)碩士學(xué)位論文 前10條

1 石文芳;基于RNA-seq測(cè)序的梅花轉(zhuǎn)錄組分析[D];北京林業(yè)大學(xué);2012年

2 楊波;基于RNA-Seq技術(shù)的長(zhǎng)江三角洲白山羊優(yōu)質(zhì)筆料毛性狀研究及皮膚毛囊結(jié)構(gòu)的觀察[D];揚(yáng)州大學(xué);2015年

3 毛笈華;利用RNA-seq技術(shù)挖掘胡蘿卜抽薹開(kāi)花相關(guān)基因[D];中國(guó)農(nóng)業(yè)科學(xué)院;2013年

4 蘇山春;基于RNA-seq技術(shù)對(duì)鼠疫耶爾森氏菌小RNA的篩選與鑒定[D];四川農(nóng)業(yè)大學(xué);2013年

5 田巍;使用多種模擬和真實(shí)數(shù)據(jù)對(duì)常用的RNA-seq比對(duì)軟件的測(cè)試與綜合比較[D];華南理工大學(xué);2015年

6 于安民;基于RNA-Seq的陽(yáng)春砂果實(shí)發(fā)育過(guò)程中糖和萜類(lèi)代謝的研究[D];廣州中醫(yī)藥大學(xué);2014年

7 張莉華;基于RNA-Seq技術(shù)的尼古丁影響下大鼠大腦中的基因表達(dá)研究[D];天津醫(yī)科大學(xué);2014年

8 趙曉俊;對(duì)羥基苯甲酸乙酯對(duì)雄果蠅的生殖毒性及其基于RNA-seq的DGE分析[D];陜西師范大學(xué);2014年

9 胡呈呈;基于RNA-seq的前列腺癌1ncRNA相關(guān)的分析研究[D];重慶醫(yī)科大學(xué);2015年

10 張清瑞;基于RNA-Seq的DHAV-1感染雛鴨的組織轉(zhuǎn)錄組分析[D];華中農(nóng)業(yè)大學(xué);2013年

，

本文編號(hào)：1605755