本文選題：β-丙氨酸　切入點(diǎn)：肌肽　出處：《新疆農(nóng)業(yè)大學(xué)》2016年碩士論文　論文類型：學(xué)位論文

【摘要】：本論文探究補(bǔ)喂β-丙氨酸對伊犁馬血漿中氨基酸含量的影響及伊犁馬運(yùn)動(dòng)水平的影響,為肌肽在運(yùn)動(dòng)馬飼養(yǎng)管理及運(yùn)動(dòng)方面提供參考。試驗(yàn)選取年齡、體重接近(平均年齡為3歲、平均體重為346.50±21.39 kg)的伊犁馬公馬12匹隨機(jī)分為2組,每組6匹,分別為α-丙氨酸組和β-丙氨酸組。所有馬匹每天飼喂苜蓿干草4 kg、優(yōu)質(zhì)山草4 kg、顆粒精料3 kg,在此基礎(chǔ)上β-丙氨酸組、α-丙氨酸組每天平均分5次分別按照馬匹體重補(bǔ)喂β-丙氨酸、α-丙氨酸300 mg/kg bw/day。試驗(yàn)一,在進(jìn)行5天預(yù)試驗(yàn)后進(jìn)行動(dòng)態(tài)點(diǎn)血樣中β-丙氨酸、α-丙氨酸及肌肽含量測定;試驗(yàn)二,給伊犁馬進(jìn)行4周β-丙氨酸補(bǔ)喂運(yùn)動(dòng)訓(xùn)練試驗(yàn),在30 d進(jìn)行模擬測試賽,并在賽前1 h、賽后即刻、賽后0.5 h、賽后2 h采集血液。試驗(yàn)結(jié)果顯示:補(bǔ)喂β-丙氨酸能顯著提高伊犁馬血漿中β-丙氨酸含量,并且血漿β-丙氨酸水平隨補(bǔ)喂量增加而上升,補(bǔ)喂8 h后上升趨勢逐漸減緩,短期補(bǔ)喂對血漿中肌肽含量影響不顯著;補(bǔ)喂30 dβ-丙氨酸能顯著提高伊犁馬運(yùn)動(dòng)水平,對運(yùn)動(dòng)前后氨基酸代謝組進(jìn)行分析后認(rèn)為,血液游離氨基酸PLS-DA得分圖顯示補(bǔ)喂β-丙氨酸4 wk對試驗(yàn)兩組運(yùn)動(dòng)前后血漿中氨基酸水平有顯著影響,對于高強(qiáng)度運(yùn)動(dòng)時(shí)伊犁馬的機(jī)體分解代謝有一定的抑制作用,并篩選出十個(gè)具有統(tǒng)計(jì)學(xué)意義的標(biāo)記物氨基酸,其中包括Val、Leu、β-ala、Ile、Car、3MHis、Lys、Eth、Arg和Tau,同時(shí)補(bǔ)喂β-丙氨酸對伊犁馬運(yùn)動(dòng)前后血?dú)狻⑸幸欢ㄓ绊?補(bǔ)喂高劑量β-丙氨酸顯著提高伊犁馬血糖水平并且對運(yùn)動(dòng)后血?dú)馑娇焖倩謴?fù)有一定作用;此外β-丙氨酸組比賽前后SOD、GSH-px、T-AOC水平顯著提高,賽后即刻氧化產(chǎn)物MDA含量極顯著下降說明補(bǔ)喂β-丙氨酸能明顯提高伊犁馬高強(qiáng)度運(yùn)動(dòng)時(shí)抗氧化水平,并且減少運(yùn)動(dòng)后損傷,加快運(yùn)動(dòng)后恢復(fù)能力。本研究結(jié)果表明,補(bǔ)充β-丙氨酸30 d能夠提高伊犁馬血漿中β-丙氨酸和肌肽含量,并且能夠增加伊犁馬運(yùn)動(dòng)水平,減少馬匹運(yùn)動(dòng)后氧化損傷,加快馬匹運(yùn)動(dòng)后恢復(fù)。
[Abstract]:In this paper, the effects of supplementation of 尾 -alanine on the content of amino acids in plasma and the exercise level of Yili horse were investigated. 12 male Yili horses with an average age of 3 years and an average body weight of 346.50 鹵21.39 kg were randomly divided into 2 groups, 6 in each group. All horses were fed with 4 kg alfalfa hay, 4 kg high quality alfalfa and 3 kg granule concentrate, respectively. On this basis, the 尾 -alanine group and 偽 -alanine group were divided into 5 times per day according to the horse body. Refeed 尾 -alanine, 偽 -alanine 300 mg/kg BW / day. test 1, After 5-day pre-test, the contents of 尾 -alanine, 偽 -alanine and carnosine in dynamic blood samples were determined. Blood samples were collected 1 hour before, immediately after the competition, 0.5 hours after the competition and 2 hours after the competition. The results showed that supplementation of 尾 -alanine could significantly increase the content of 尾 -alanine in the plasma of Yili horse, and the plasma 尾 -alanine level increased with the increase of supplemental feeding. After 8 hours of supplementation, the increasing trend was gradually slowed down, and the effect of short-term supplementation on plasma carnosine content was not significant, and the 尾 -alanine supplementation for 30 days significantly increased the exercise level of Yili horse. The results showed that the amino acid metabolism group before and after exercise was analyzed. The PLS-DA score of free amino acids in blood showed that supplementation of 尾 -alanine for 4 wk significantly affected the level of plasma amino acids before and after exercise in both groups, and inhibited the catabolism of Yili horse during high intensity exercise. Ten marked amino acids were screened, including Valen Leu, 尾 -Ala Ile Carr 3M HislysTaug and Tau.The supplementation of 尾 -alanine had a certain effect on blood gas and biochemistry of Yili horse before and after exercise. Supplementation of high dose 尾 -alanine significantly increased the blood glucose level of Yili horse and played a certain role in the rapid recovery of blood gas level after exercise, in addition, the level of GSH-pxX T-AOC in 尾 -alanine group was significantly increased before and after the competition. The extremely significant decrease in the content of MDA showed that supplementation of 尾 -alanine could significantly increase the antioxidant level, reduce the injury after exercise and accelerate the ability of recovery after exercise in Yili horse during high intensity exercise, the results showed that 尾 -alanine supplementation significantly increased the antioxidant activity of Yili horse during high intensity exercise. Supplementation of 尾 -alanine for 30 days could increase plasma 尾 -alanine and carnosine content in Yili horse, increase the exercise level of Yili horse, reduce oxidative injury after exercise, and accelerate the recovery of horses after exercise.
【學(xué)位授予單位】：新疆農(nóng)業(yè)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2016
【分類號】：S821.5

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