發(fā)布時(shí)間：2018-03-07 02:14

  本文選題：新疆哈薩克綿羊　切入點(diǎn)：主要組織相容性復(fù)合體(MHC)Ⅰ類分子基因　出處：《黑龍江畜牧獸醫(yī)》2017年17期 　論文類型：期刊論文

【摘要】：為了研究新疆哈薩克綿羊主要組織相容性復(fù)合體(MHC)Ⅰ類分子基因序列的多態(tài)性,采用PCR和測(cè)序的方法分別克隆新疆哈薩克綿羊MHCⅠ類分子基因重鏈和輕鏈的c DNA全長(zhǎng),并進(jìn)行生物信息學(xué)分析。結(jié)果表明:成功擴(kuò)增出新疆哈薩克綿羊MHCⅠ類分子基因重鏈、輕鏈,大小分別為1 107 bp、357 bp;通過(guò)生物信息學(xué)軟件預(yù)測(cè)蛋白質(zhì)分子質(zhì)量分別為90 ku和29.694 ku;等電點(diǎn)(p I)分別為5.00,5.23;重鏈富含G、C堿基,有兩段跨膜螺旋,最大疏水指數(shù)為3.622,最小疏水指數(shù)為-2.922,預(yù)測(cè)整條肽鏈表現(xiàn)為親水性,在1~84位堿基之間存在信號(hào)肽;輕鏈四種堿基含量基本一致,也有兩段跨膜螺旋,在1~63位堿基之間存在信號(hào)肽。新疆哈薩克綿羊MHCⅠ類分子基因編碼蛋白質(zhì)的二級(jí)結(jié)構(gòu)以無(wú)規(guī)則卷曲元件的含量最高,該蛋白質(zhì)的三級(jí)結(jié)構(gòu)由一條α鏈,另一條為β鏈通過(guò)非共價(jià)鍵組成,由α1、α2組成抗原結(jié)合槽;NCBI數(shù)據(jù)庫(kù)中不同地區(qū)的哈薩克綿羊的MHCⅠ類分子基因的同源性在89.87%~99.91%之間,與數(shù)據(jù)庫(kù)中牛的MHCⅠ類分子基因同源性達(dá)到了98.88%,輕鏈MHCⅠ類分子基因還與豬和人的同源性相對(duì)較高。
[Abstract]:In order to study the polymorphism of class I gene sequence of Xinjiang Kazak sheep main histocompatibility complex (MHC), the length of c DNA of MHC class I heavy chain and light chain of Xinjiang Kazak sheep was cloned by PCR and sequencing, respectively. The results of bioinformatics analysis showed that the heavy chain and light chain of MHC class I gene in Xinjiang Kazakh sheep were successfully amplified. The molecular weight of the protein was estimated by bioinformatics software to be 90 ku and 29.6ku94, the isoelectric point I was 5.00 ~ 5.23, respectively. The heavy chain was rich in G ~ (+) C base and had two transmembrane helices. The maximum hydrophobic index was 3.622, and the minimum hydrophobic index was -2.922. It was predicted that the whole peptide chain was hydrophilic, and there were signal peptides between the 1 ~ 84 bases, and the four bases in the light chain were basically the same, and there were also two transmembrane helices. In Xinjiang Kazak sheep, the secondary structure of the protein encoded by MHC class I gene was the highest in the irregular curl element, and the tertiary structure of the protein consisted of a 偽 chain. The other is that the 尾 chain is composed by non-covalent bonds, and the homology of MHC class I gene of Kazakh sheep in different regions in the database of 偽 1, 偽 2 antigen-binding trough is between 89.87% and 99.91%. The homology of bovine MHC class I gene was 98.88, and that of light chain MHC class I gene was higher than that of pig and human.
【作者單位】： 石河子大學(xué)動(dòng)物科技學(xué)院;
【基金】：國(guó)家自然科學(xué)基金項(xiàng)目(31172303)
【分類號(hào)】：S826
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本文編號(hào)：1577492