本文選題：豬瘟　切入點：豬繁殖與呼吸障礙綜合征　出處：《上海交通大學(xué)》2015年碩士論文　論文類型：學(xué)位論文

【摘要】：豬瘟(Classical swine fever,CSF)、豬繁殖與呼吸障礙綜合征(Porcine reproductive and respiratory syndrome,PRRS)是全世界養(yǎng)豬業(yè)危害較大的兩大疫病。本研究選取大型豬場內(nèi)豬群作為研究對象,進(jìn)行豬場定期CSF和PRRS血清抗體水平監(jiān)測,并通過不同的免疫途徑,檢測抗體效價結(jié)果,確定更合適的免疫程序,以期為疫病的防控和流行病學(xué)調(diào)查提供科學(xué)依據(jù)。本研究于2013年-2014年對某大型豬場血清用CSF和PRRS相應(yīng)的抗體或抗原檢測試劑盒檢測分析了兩個不同免疫程序下的豬群免疫抗體水平和感染情況。2014年3月前CSF按6周齡首免,8周齡二免,28周齡三免。此階段,我們每個月隨即采取4周齡仔豬、8周齡和11周齡仔豬血液各30份進(jìn)行CSF抗原抗體檢測,共檢測了5次,結(jié)果4周齡仔豬豬瘟母源抗體平均陽性率和抗原平均陽性率分別為36.00%和6.67%,8周齡和11周齡仔豬豬瘟抗體平均陽性率和豬瘟抗原平均陽性率分別為47.03%、60.67%和1.33%、0%;2014年3月后調(diào)整免疫將首免提前至4周齡,7周齡二免,28周齡三免。此階段,我們每個月隨即采取免疫前4周齡仔豬、6周齡和10周齡仔豬血液各30份進(jìn)行CSF抗原抗體檢測,共檢測了5次,結(jié)果4周齡仔豬豬瘟母源抗體平均陽性率和抗原平均陽性率分別為36.66%和0.67%,6周齡和10周齡仔豬豬瘟抗體平均陽性率和豬瘟抗原平均陽性率分別為62.00%、70.67%和0%、0%。統(tǒng)計學(xué)分析表明,兩種免疫效果組間差異顯著,其中,4周齡的血清檢測兩種免疫程序差異不顯著(P0.05),而其他兩個階段的檢測結(jié)果,4周齡首免要優(yōu)于6周齡首免(P0.05),說明豬瘟疫苗首免選擇在仔豬28日齡左右效果好。同時PRRS采用兩種免疫程序進(jìn)行免疫效果試驗,4周齡首免PRRS疫苗的仔豬,15周齡和33周齡PRRS抗體平均陽性率分別為85.33%和91.33%;1周齡首免PRRS疫苗的仔豬,15周齡和33周齡時PRRS抗體平均陽性率分別為94.67%和98.00%。兩者差異不顯著(P0.05),該豬場PRRS疫苗免疫效果比較理想。
[Abstract]:Classical swine fever (Classical swine fever, CSF), porcine reproductive and respiratory syndrome (Porcine reproductive and respiratory syndrome, PRRS) is the world's two major diseases in pig industry harm is greater. This study selected large farms pigs as the research object, to set the CSF and pig serum antibody level of PRRS monitoring, and through immunization the detection of antibody titer results, determine the appropriate immunization program, to provide a scientific basis for the prevention and control of epidemic disease and epidemiological investigation. The research on 2013 -2014 years of a large pig serum CSF and PRRS with the corresponding antibody or antigen detection kit for detection and analysis of two different immune procedures under the swine antibody and the level of.2014 infection in March before the CSF according to the age of 6 weeks from the first 8 weeks of age, two free, 28 weeks of age three free. In this period, every month we took 4 week old piglets, 8 weeks and 11 weeks The blood 30 copies of detection of CSF antigen and antibody were detected, 5 times, the average positive rate of 4 week old piglets swine fever maternal antibody antigen and the average positive rate were 36% and 6.67%, the average positive rate of 8 week old and 11 week old piglets swine fever antibody and the average positive rate of swine fever antigen respectively 47.03%, 60.67% and 1.33%, 0%; after March 2014 will be the first free adjustment of the immune advance to 4 weeks, 7 weeks and two free, 28 week old three free. At this stage, we take each month immediately before immunization of piglets with 4 weeks, 6 weeks and 10 week old piglets were detected 30 CSF antigen antibodies were detected in 5 times, the average positive rate of 4 week old piglets swine fever maternal antibody antigen and the average positive rate were 36.66% and 0.67%, the average positive rate of 6 week old and 10 week old piglets swine fever antibody and the average positive rate of swine fever antigen were 62%, 70.67% and 0%, 0%. statistical analysis table Ming, two free The difference between the groups was plague, the serum of 4 week old two immunization program had no significant difference (P0.05), while the other two stages of the test results, the first 4 weeks than 6 weeks of age the first free free (P0.05), that the first free choice of swine fever vaccine in piglets of 28 days effect at the same time. PRRS uses two kinds of immunization program were immune effect test, 4 week old the first free PRRS vaccine of piglets, the average positive rate of 15 and 33 weeks old PRRS antibody were 85.33% and 91.33%; 1 weeks old the first free PRRS vaccine of piglets, 15 and 33 weeks of age the average positive rate of PRRS both antibodies were 94.67% and 98.00%., the difference was not significant (P0.05), the pig PRRS vaccine effect is ideal.

【學(xué)位授予單位】：上海交通大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：S858.28

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