本文選題：保育豬　切入點：病原學　出處：《江西農(nóng)業(yè)大學》2015年碩士論文　論文類型：學位論文

【摘要】：目前,疫病是影響江西養(yǎng)豬業(yè)的重要因素之一,養(yǎng)豬生產(chǎn)上面臨著疫病種類多、病原變異、多病原混合感染等問題。隨著日齡的增長,母源抗體水平逐漸下降、斷奶應激、疫苗免疫保護未完全形成等因素,保育豬是規(guī)�；B(yǎng)豬場中最容易發(fā)生疫病(尤其是多病原感染)的豬群。為了解江西地區(qū)保育豬的病原的感染及變異情況,本論文從江西部分豬場采集病(死)保育豬病料,采分子生物學方法分析豬瘟病毒(CSFV)、豬繁殖與呼吸綜合征病毒(PRRSV)、豬圓環(huán)病毒2型(PCV2)和豬偽狂犬病毒(PRV)四種病毒的感染情況,隨機對部分毒株進行測序,分別了解病原的變異情況,為江西地區(qū)養(yǎng)豬場進行保育豬的疫病防控提供病原學依據(jù)。1.江西地區(qū)保育豬的病原感染情況調(diào)查2014年1月至2014年12月間,從江西省南昌、宜春、吉安、贛州、撫州、九江和上饒地區(qū)的不同規(guī)模養(yǎng)豬場采集546份病(死)保育豬病料,對每一份病料均分別進行了豬繁殖與呼吸綜合征病毒(PRRSV)、豬瘟病毒(CSFV)、豬圓環(huán)病毒2型(PCV2)和豬偽狂犬病毒(PRV)四種病病毒的PCR/RT-PCR檢測。結果顯示:PRRSV的陽性數(shù)為375,占總樣品百分率為68.6%;CSFV的陽性數(shù)為204,占總樣品百分率為37.3%;PCV2的陽性數(shù)為275,占總樣品百分率為50.3%;PRV的陽性數(shù)為70,占總樣品百分率為12.8%。2.四種病原的感染類型分析546份病(死)保育病料樣品中,單獨感染的樣品為203份,占總樣品數(shù)的37.1%;二重混合感染的樣品為308份,占總樣品數(shù)的56.3%;三重感染的樣品為30份,占總樣品數(shù)的5.4%;四重感染的樣品為5份,占總樣品數(shù)的0.9%。調(diào)查結果表明:保育豬的病原的多重感染普遍化,PRRSV、CSFV、PCV2和PRV的二重以上混合感染高達62.9%;二重感染中,以“PRRSV+PCV2”或“PRRSV+CSFV”感染為主,分別占二重混合感染的45.1%和31.8%。3.PRRSV、CSFV、PCV2和PRV的變異情況分析(1)12株豬瘟病毒江西流行株的E2基因的核苷酸和氨基酸同源性分別為96.5%-100%和92.4%-100%;江西流行株與CSFV石門株的核苷酸同源性為82.6%-83.3%。通過E2基因的遺傳進化樹分析中,可將CSFV可分為兩大基因群,即基因1群和基因2群,本論文測定的12株豬瘟病毒江西流行株均屬于基因2群的2.1b亞群。(2)15株PRRSV毒株ORF5基因的核苷酸和氨基酸同源性為93.7%-99.7%和89.1%-100%。與美洲株(VR2332)的核苷酸和氨基酸同源性為82.4%-87.2%和80.2%-89.1%,與歐洲株(MLV)的核苷酸和氨基酸同源性為79.7%-86.6%和82.1%-86.9%。15株PRRSV江西流行株均屬于美洲型。(3)11株PCV2江西流行株的核苷酸和氨基酸同源性為94.2%-99.7%和64.2%-93.2%。遺傳進化樹分析結果表明:11株江西毒流行株中9株屬于PCV2b型,2株屬于PCV2a型。(4)9株PRV江西流行株gB基因的核苷酸和氨基酸同源性分別為94.7%-100%和99.5%-99.9%。與BARTHA-2011相比,核苷酸同源性為97.8%-97.9%,與KAPLAN相比,同源性為98.0%-98.2%。遺傳進化樹分析,9株流行株親緣關系近。
[Abstract]:At present, epidemic disease is one of the important factors affecting the pig industry in Jiangxi Province. Pig production is faced with many kinds of disease, variation of pathogen, mixed infection of multiple pathogens, and so on. With the increase of age, the level of maternal antibody decreases gradually, and the weaning stress. The vaccine immunity protection is not fully formed, and the care of pigs is the most susceptible to blight disease (especially multi-pathogen infection) in large-scale pig farms. In order to understand the infection and variation of the pathogen of the preserved pigs in Jiangxi area, In this paper, four kinds of viruses, CSFV, PRRSVV, PCV2 and PRV), were collected from some pig farms in Jiangxi province. The infection of CSFV, PRRSVV, PCV2 and PRV) were analyzed by molecular biological methods. Some virus strains were sequenced at random to find out the variation of pathogen, and to provide the etiological basis for the prevention and control of pig diseases in Jiangxi pig farms. 1. The investigation of pathogen infection of preserved pigs in Jiangxi area from January 2014 to December 2014. 546 samples were collected from pig farms of different scales in Nanchang, Yichun, Ji'an, Ganzhou, Fuzhou, Jiujiang and Shangrao regions of Jiangxi Province. PCR/RT-PCR detection of PRRSVV, CSFV, PCV2 and PRV) of porcine reproductive and respiratory syndrome virus (PRRSVV) and porcine pseudorabies virus (PRV2) were carried out respectively. The results showed that the positive number of the virus was 375, accounting for the total sample. The positive rate of CSFV was 204, the percentage of PCV2 was 275, the percentage of PCV2 was 50.3%, the positive number of PRV was 70, and the percentage of total samples was 12.8.2.The infection types of the four pathogens were analyzed in 546 samples. The number of samples infected alone was 203, accounting for 37.1of the total number of samples; the number of samples with double mixed infection was 308, accounting for 56.3% of the total number of samples; the number of samples with triple infection was 30, accounting for 5.4% of the total number of samples; and the number of samples with quadruple infection was 5. The results of the investigation showed that the double infection of PRRSVV, CSFV, PCV2 and PRV was as high as 62.9%, and in the double infection, "PRRSV PCV2" or "PRRSV CSFV" was the main infection. 45.1% and 31.8.3.The variation of PRV and CSFVV2 of PRRSVV virus in Jiangxi Province the nucleotide and amino acid homology of E2 gene of 12 strains of swine fever virus in Jiangxi were 96.5-100% and 92.4-100%, respectively; the nucleotide homology between the prevalent strain of Jiangxi and CSFV strain was 96.5% -100% and 92.4-100%, respectively; the nucleotide homology between the epidemic strain of Jiangxi and the strain of CSFV Shimen strain was 96.5% -100% and 92.4-100%, respectively. The sex is 82.6% -83.3%. In the genetic evolution tree analysis of E2 gene, CSFV can be divided into two groups: group 1 and group 2. In this paper, the nucleotide and amino acid homology of the ORF5 gene of all the 12 prevalent strains of CSFV in Jiangxi were 93.7-99.7% and 89.1-100, respectively, and the homology of nucleotide and amino acid were 82.4- 87.2% and 82.4- 87.2%, respectively, compared with that of the American strain VR2332. The nucleotide and amino acid homology of the ORF5 gene of all the 12 strains of CSFV were 2.1b, 2.1b, 2.1b, 2.1b and 2.1b, respectively. The homology of nucleotide and amino acids with the European strain MLV was 79.7-86.6% and 82.1 -86.9.15 strains of PRRSV Jiangxi were of American type. The nucleotide and amino acid homology of Jiangxi strain were 94.2-99.7% and 64.2-99.7% respectively. The results of genetic evolution tree analysis showed that the genetic evolution tree analysis showed that the genetic evolution tree analysis showed that the nucleotide and amino acid homology was 94.2 -99.7% and 64.2% -29.3%, respectively. The results of genetic phylogenetic tree analysis showed that the nucleotide and amino acid homology was 94.2% -99.7% and 64.2% -29.3% respectively. The nucleotide and amino acid homology of GB gene in 9 strains of Jiangxi virulent strain belonging to PCV2b type, 2 strains belonging to PCV2a type and 9 strains of PRV epidemic strain in Jiangxi province were 94.7- 100% and 99.5- 99.9%, respectively. Compared with BARTHA-2011, the nucleotide and amino acid homology of GB gene in Jiangxi epidemic strain were 94.7% -100% and 99.5- 99.9%, respectively. The nucleotide homology was 97.8- 97.99.Compared with KAPLAN, the homology was 98.0-98.2.The genetic phylogenetic tree analysis showed that 9 epidemic strains were close to each other.
【學位授予單位】：江西農(nóng)業(yè)大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：S858.28

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