發(fā)布時(shí)間：2018-03-03 18:23

  本文選題：桑葉　切入點(diǎn)：陜北白絨山羊　出處：《西北農(nóng)林科技大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：目前關(guān)于桑葉作為飼料資源的研究多局限于日糧中添加桑葉對(duì)動(dòng)物生長、生產(chǎn)性能的影響,而關(guān)于桑葉對(duì)動(dòng)物脂肪代謝相關(guān)基因表達(dá)的影響少有報(bào)道。因此,本試驗(yàn)以陜北白絨山羊?yàn)檠芯繉?duì)象,探討了日糧中桑葉不同添加方式和添加量對(duì)絨山羊血清生化指標(biāo)及脂肪代謝相關(guān)基因LPL、FASN、Leptin mRNA表達(dá)量和蛋白表達(dá)量的影響,旨在研究桑葉對(duì)絨山羊脂肪代謝的影響,為桑葉在絨山羊飼養(yǎng)中的開發(fā)利用提供理論基礎(chǔ)。選用體況良好(平均重量28±1.05 kg)的陜北白絨山羊羯羊60只,隨機(jī)均分為6組(對(duì)照組、干桑葉組Ⅰ、干桑葉組Ⅱ、青貯桑葉組Ⅰ、青貯桑葉組Ⅱ和青貯桑葉組Ⅲ)。對(duì)照組日糧不添加桑葉,干桑葉組Ⅰ和干桑葉組Ⅱ分別添加10%、15%的干桑葉,青貯桑葉組Ⅰ、青貯桑葉組Ⅱ和青貯桑葉組Ⅲ分別添加10%、15%和20%的青貯桑葉。飼養(yǎng)試驗(yàn)持續(xù)70 d。飼養(yǎng)試驗(yàn)結(jié)束后,每組隨機(jī)挑選3只羊頸部靜脈采血,屠宰采集腹部皮下脂肪、腎周脂肪、尾部脂肪、肝臟、背最長肌組織樣。測(cè)定血清生化指標(biāo);通過實(shí)時(shí)熒光定量PCR檢測(cè)各組織中LPL、FASN、Leptin mRNA表達(dá)量;通過Western Blot檢測(cè)各組織中LPL、FASN、Leptin蛋白表達(dá)量。主要研究結(jié)果如下:(1)與對(duì)照組相比,飼喂桑葉對(duì)血清中HDL-C、TC、TG、LPS、LPL濃度的影響均不顯著(P0.05);青貯桑葉組Ⅱ和干桑葉組Ⅱ的血清LDL-C濃度分別顯著降低30%和36.4%(P0.05);飼喂干桑葉顯著降低絨山羊血清Leptin濃度(P0.05);干桑葉組Ⅱ和青貯桑葉組Ⅲ中血清FASN濃度均顯著降低(P0.05)。(2)肝臟中LPLmRNA表達(dá)量顯著低于腹部皮下脂肪、尾部脂肪和腎周脂肪(P0.05),但脂肪組織間LPL mRNA表達(dá)無顯著差異(P0.05);FASN在肝臟中mRNA表達(dá)量最高,除尾部脂肪和腎周脂肪外,其他組織間FASNmRNA表達(dá)量均有顯著差異(P0.05);LeptinmRNA在腹部皮下脂肪中表達(dá)量最高。(3)在肝臟中,干桑葉組Ⅱ和青貯桑葉組Ⅱ中FASN基因表達(dá)量顯著降低(P0.05);腹部皮下脂肪中,青貯桑葉組Ⅲ的LPL和Leptin基因表達(dá)量顯著增加(P0.05);腎周脂肪中,LPL基因表達(dá)量在青貯桑葉組Ⅱ中顯著上升(P0.05);尾部脂肪中,干桑葉組Ⅱ和青貯桑葉組Ⅱ的LPL基因表達(dá)量顯著增加(P0.05),干桑葉組Ⅱ和青貯桑葉組Ⅲ的Leptin表達(dá)量顯著增加(P0.05);背最長肌中,青貯桑葉組Ⅱ的LPL和Leptin基因表達(dá)量顯著上升(P0.05)。(4)LPL和Leptin在腹部皮下脂肪、尾部脂肪、腎周脂肪、肝臟中蛋白表達(dá)量兩兩均差異顯著(P0.05);FASN蛋白在尾部脂肪中表達(dá)量顯著高于其他3種組織(P0.05),腎周脂肪中FASN蛋白表達(dá)量顯著高于腹部皮下脂肪和肝臟(P0.05)。(5)在肝臟中,飼喂干桑葉或青貯桑葉均顯著降低LPL蛋白表達(dá)量(P0.05);除干桑葉組Ⅱ外,其余組Leptin蛋白表達(dá)量顯著升高(P0.05)。腹部皮下脂肪中,飼喂干桑葉和青貯桑葉LPL蛋白表達(dá)量均顯著降低(P0.05);青貯桑葉組Ⅱ中FASN蛋白表達(dá)量顯著降低(P0.05),且Leptin蛋白表達(dá)量顯著升高(P0.05)。腎周脂肪中,除干桑葉組Ⅰ外,其余各組LPL蛋白表達(dá)量均顯著降低(P0.05);干桑葉組Ⅰ、干桑葉組Ⅱ和青貯桑葉組Ⅱ中FASN蛋白表達(dá)量顯著降低(P0.05),但Leptin蛋白表達(dá)量顯著增加(P0.05)。尾部脂肪中,飼喂青貯桑葉顯著降低LPL和FASN蛋白表達(dá)量(P0.05),同時(shí)顯著促進(jìn)Leptin蛋白表達(dá)(P0.05)。背最長肌中,干桑葉組Ⅰ的LPL和Leptin蛋白表達(dá)量均顯著升高(P0.05)。綜上所述,LPL、FASN和Leptin在絨山羊各組織中mRNA表達(dá)和蛋白表達(dá)模式并不一致。在絨山羊日糧中添加15%的青貯桑葉可有效抑制脂肪沉積,能夠促進(jìn)脂肪組織中LPL和LeptinmRNA表達(dá),抑制肝臟中FASN mRNA表達(dá);添加15%的青貯桑葉提高脂肪組織中Leptin蛋白表達(dá)量,降低脂肪組織中LPL和FASN蛋白表達(dá)量。
[Abstract]:The current research on the mulberry leaves as feed resources are confined to the diets of mulberry leaves on animal growth, affect the production performance, and the effects of mulberry leaf on expression of genes related to animal fat metabolism is rarely reported. Therefore, this experiment in Shanbei white cashmere goat as the research object, discusses the mulberry leaf diets with different adding ways and add the amount of cashmere goat serum biochemical indexes and lipid metabolism related genes LPL, FASN, Leptin mRNA expression and protein, to study effect of mulberry leaves on Cashmere Goat fat metabolism, and provide a theoretical basis for the development and utilization of mulberry leaves in cashmere goat breeding. With good physical condition (the average weight of 28 + 1.05 kg Shanbei White Cashmere Goat wethers) 60 rats were randomly divided into 6 groups (control group, stem leaf group I and group II dry mulberry leaves, Mulberry Leaf Silage silage group I, group II and group III silage mulberry leaves). The control group diet not Tim Gasanje, the group I dried mulberry leaves and dry leaves in group II were added 10%, 15% dried mulberry leaves, Mulberry Leaf Silage silage group I, group II and group III were added 10% Mulberry Leaf Silage, silage mulberry 15% and 20%. The feeding experiment lasted for 70 D. after the end of the feeding trial, each randomly selected 3 sheep neck vein blood slaughter collection, abdominal subcutaneous fat, perirenal fat, fat tail, liver and longissimus muscle tissue samples. The determination of serum biochemical indexes; through the organization of real time fluorescence quantitative PCR detection of LPL, FASN, expression of Leptin mRNA by Western Blot; the detection of LPL, FASN, Leptin protein expression. The main research the results are as follows: (1) compared with the control group, fed with mulberry leaf on serum HDL-C, TC, TG, LPS, LPL concentration were not significant (P0.05); group II and Mulberry Leaf Silage dried mulberry leaf group serum LDL-C concentrations were significantly decreased by 30% and 36.4% (P0.05); feeding dry mulberry leaves Leptin significantly reduced the serum concentration of cashmere goat (P0.05); group II and Mulberry Leaf Silage dry mulberry leaves in group III were significantly decreased the concentration of serum FASN (P0.05). (2) was significantly lower than the expression of LPLmRNA in abdominal subcutaneous fat liver, tail fat and perirenal fat (P0.05), but the fat tissue LPL mRNA expression significant differences (P0.05); FASN mRNA expression was the highest in liver, except the tail fat and perirenal fat, the expression amount of difference were FASNmRNA other organizations (P0.05); LeptinmRNA in abdominal subcutaneous fat, the highest expression level. (3) in the liver, dry mulberry leaves FASN group II and group II Mulberry Leaf Silage gene expression was significantly decreased (P0.05); abdominal subcutaneous fat, significantly increased the expression of LPL and Leptin gene of Mulberry Leaf Silage group III (P0.05); kidney fat, LPL gene expression increased significantly in group II in Mulberry Leaf Silage (P0.05); tail fat, dry mulberry leaves and group II A significant increase in the amount of expression of LPL gene of Mulberry Leaf Silage group (P0.05), dried mulberry leaf group II and group III of the Mulberry Leaf Silage Leptin expression was significantly increased (P0.05); longissimus muscle, significantly increased the expression of LPL and Leptin gene of Mulberry Leaf Silage group (P0.05). (4) LPL and Leptin in abdominal subcutaneous fat, tail fat, perirenal fat, liver protein 22 expression levels were significantly different (P0.05); the expression of FASN protein in tail fat were significantly higher than the other 3 groups (P0.05), was significantly higher than that in subcutaneous abdominal fat and liver FASN protein expression in kidney fat (5 (P0.05).) in the liver, feeding mulberry leaves or stem silage mulberry leaves were significantly decreased the expression of LPL protein (P0.05); group II in addition to dry mulberry leaves, the remaining group Leptin protein expression increased significantly (P0.05). Abdominal subcutaneous fat, dry leaves and Mulberry Leaf Silage feeding LPL protein expression was significantly decreased (P0.05); silage mulberry Leaf group II FASN protein expression was significantly decreased (P0.05), and Leptin protein expression increased significantly (P0.05). The perirenal fat, in addition to dry mulberry group, other groups the expression of LPL protein were significantly decreased (P0.05); group I dry mulberry leaves, mulberry and Mulberry Leaf Silage dry group II, group II FASN protein expression was significantly decreased (P0.05), but the expression of Leptin protein increased significantly (P0.05). Tail fat, silage feeding mulberry leaves significantly reduced LPL and FASN protein expression (P0.05), and significantly increase the expression of Leptin (P0.05). Longissimus muscle, dry mulberry leaves in group I and LPL the expression of Leptin protein were significantly increased (P0.05). To sum up, LPL, FASN and Leptin expression in various tissues and the expression of mRNA protein in goat model is not consistent. Add in silage mulberry leaves 15% cashmere goat diets can effectively inhibit fat deposition, can promote LPL and LeptinmRNA in fat tissue The expression of FASN mRNA in the liver was inhibited, and 15% of the silage mulberry leaves increased the expression of Leptin protein in the adipose tissue and reduced the expression of LPL and FASN protein in the adipose tissue.

【學(xué)位授予單位】：西北農(nóng)林科技大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：S827.5

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4 周英昊;陜北白絨山羊堿性氨基酸轉(zhuǎn)運(yùn)載體基因cDNA克隆及時(shí)空表達(dá)分析[D];西北農(nóng)林科技大學(xué);2015年

5 許鑫;光照控制對(duì)陜北白絨山羊絨毛生長的影響研究[D];西北農(nóng)林科技大學(xué);2015年

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8 鮑文;陜北白絨山羊與灘羊的虛擬水需要量及其比較分析研究[D];西北農(nóng)林科技大學(xué);2016年

9 林昌龍;舍飼條件下陜北白絨山羊光控增絨技術(shù)的研究[D];西北農(nóng)林科技大學(xué);2016年

10 王丹;內(nèi)蒙古阿爾巴斯白絨山羊食毛癥與肺炎的診斷研究[D];內(nèi)蒙古農(nóng)業(yè)大學(xué);2017年

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