本文選題：雞　切入點：精液　出處：《畜牧獸醫(yī)學報》2017年04期 　論文類型：期刊論文

【摘要】：本研究旨在探索稀釋和低溫保存處理對雞精液品質(zhì)和受精能力的影響,以優(yōu)化雞精液的短期保存條件。試驗采集30只北京油雞精液,混勻后等量分為4組:A組精液不處理作為對照,B組用BPSE稀釋液按照1∶1比例稀釋,C組精液離心后,去除上層精漿,加入與精漿等體積的BPSE稀釋液重新混勻,D組在C組處理的基礎上再用BPSE稀釋液按照1∶1比例稀釋。每組精液分為兩份,一份用于直接輸精和精液品質(zhì)測定,另一份在4℃保存,期間每隔8h檢測一次精液品質(zhì),24h后輸精,每組收集種蛋120枚左右,統(tǒng)計受精率和孵化率等指標。結果表明:不同稀釋處理后,C和D組精子活力極顯著低于A組對照和B組(P0.01),B組和C組的受精率、入孵蛋的孵化率和受精蛋的孵化率與A組無顯著差異,但是D組3項指標均極顯著低于A組對照(P0.01)。隨著4℃保存時間的延長,4組精子活力均極顯著降低(P0.01),其中C和D組降低的幅度顯著低于A組對照和B組。精子畸形率隨著低溫保存時間的延長而顯著增加(P0.05),且畸形主要表現(xiàn)為頭部腫脹,頸部彎曲和尾部斷裂。與直接輸精相比,低溫保存24h后,A、B和C組的受精率和孵化率都顯著降低(P0.05),但是D組低溫保存前后的受精率和孵化率差異均不顯著(P0.05),分別可以達到71%和82%。綜上表明,新鮮精液可以進行1∶1稀釋后輸精,在不影響受精率的前提下可以提高公雞配種能力。在需要短期保存精液后進行輸精時,可以將采集的新鮮精液離心去除精漿并進行稀釋,保存于4℃,從而降低精子活力的下降幅度,維持受精能力。
[Abstract]:The purpose of this study was to explore the effects of dilution and cryopreservation on the quality and fertilization ability of chicken semen in order to optimize the short-term preservation conditions of chicken semen. After blending, the semen of group B was divided into 4 groups: group A, without treatment, and group B, with BPSE diluting the semen of group C according to the ratio of 1: 1, after centrifugation, the upper seminal plasma was removed. Group D was remixed with seminal plasma of equal volume of BPSE diluent and then diluted with BPSE diluent in proportion of 1: 1 on the basis of group C. Each group of semen was divided into two parts, one for direct insemination and determination of semen quality, the other for preservation at 4 鈩，

本文編號：1560755