本文關(guān)鍵詞： 南美蟛蜞菊 葉蛋白 提取方法　出處：《南方農(nóng)業(yè)學(xué)報(bào)》2017年07期 　論文類型：期刊論文

【摘要】：【目的】篩選出適合南美蟛蜞菊葉蛋白的提取方法和條件,為其蛋白飼料資源化利用提供技術(shù)支持。【方法】通過(guò)正交試驗(yàn)分別優(yōu)化酸加熱法、堿加熱法、鹽析法和有機(jī)溶劑法提取南美蟛蜞菊葉蛋白的工藝條件,并對(duì)比4種方法的提取效果,確定最佳提取工藝。【結(jié)果】4種方法提取的南美蟛蜞菊葉片粗蛋白含量排序?yàn)?鹽析法(15.18 mg/g)酸加熱法(9.97 mg/g)堿加熱法(9.79 mg/g)有機(jī)溶劑法(7.21 mg/g)。其中,鹽析法的最適提取條件為氯化鈉質(zhì)量濃度30%、絮凝時(shí)間10 min;酸加熱法的最適提取條件為絮凝溫度60℃、pH 3、絮凝時(shí)間2 min;堿加熱法的最適提取條件為絮凝溫度25℃、pH 7、絮凝時(shí)間2 min;有機(jī)溶劑法的最適提取條件為乙醇體積分?jǐn)?shù)20%、絮凝時(shí)間10 min。絮凝溫度對(duì)酸加熱法和堿加熱法提取效率有顯著影響(P0.05),溶液濃度對(duì)鹽析法和有機(jī)溶劑法提取效率有極顯著影響(P0.01)。【結(jié)論】綜合比較4種方法提取南美蟛蜞菊葉蛋白的效果,確定鹽析法是提取南美蟛蜞菊葉蛋白的最適方法,其提取效率高、操作簡(jiǎn)便、蛋白質(zhì)活性保持較好,適用于實(shí)驗(yàn)室提取和工業(yè)生產(chǎn)。
[Abstract]:[objective] to screen the extraction methods and conditions suitable for wedelia chinensis leaf protein, and to provide technical support for the utilization of protein feed. [methods] the acid heating method and alkali heating method were optimized by orthogonal experiment. The extraction conditions of wedelia chinensis leaf protein by salting out method and organic solvent method were compared. [results] the order of crude protein content in leaves of Wedelia trilobata was: salting-out method 15.18 mg / g) acid heating method 9.97 mg / g) alkali heating method 9.79 mg / g organic solvent method 7.21 mg / g. The optimum extraction conditions of salting-out method are as follows: sodium chloride concentration 30, flocculation time 10 min; acid heating extraction condition: flocculation temperature 60 鈩，

本文編號(hào)：1544777