本文關(guān)鍵詞： 卵透明帶 DNA疫苗 電脈沖法 免疫不育　出處：《中國免疫學(xué)雜志》2017年02期 　論文類型：期刊論文

【摘要】：目的:研究電脈沖法對(duì)CZP3 DNA疫苗免疫不育效果的影響。方法:在小鼠腿部肌肉分別注射5、10、20和50μg的pc DNA3.0-CZP3質(zhì)粒,經(jīng)電脈沖刺激,ELISA法檢測(cè)免疫小鼠的CZP3抗體水平及最高抗體滴度。與雄鼠合籠三周后,計(jì)算各免疫組的不孕率及平均產(chǎn)仔數(shù),統(tǒng)計(jì)學(xué)分析。結(jié)果:相比單獨(dú)肌肉注射免疫,電脈沖法能極顯著提高DNA疫苗的免疫水平,電脈沖50μg組的抗體效價(jià)可以達(dá)到1∶4 000。此外隨著血清中CZP3抗體水平的增高,小鼠不孕率明顯上升,平均產(chǎn)仔數(shù)顯著減少。統(tǒng)計(jì)分析顯示,免疫小鼠的CZP3抗體水平與產(chǎn)仔數(shù)呈負(fù)相關(guān)關(guān)系。結(jié)論:電脈沖法能夠顯著提高CZP3 DNA疫苗的免疫水平,降低小鼠生育能力,這為CZP3 DNA疫苗應(yīng)用于流浪犬避孕奠定了基礎(chǔ)。
[Abstract]:Objective: to study the effect of electric pulse method on immune infertility of CZP3 DNA vaccine. Methods: 50 渭 g and 50 渭 g PC DNA3.0-CZP3 plasmids were injected intramuscularly into the leg of mice. The CZP3 antibody level and the highest antibody titer of immunized mice were detected by electric pulse stimulation Elisa method. After three weeks of cage with male mice, the infertility rate and average litter size of each immune group were calculated and statistically analyzed. Results: compared with single intramuscular immunization, the mice were immunized by intramuscular injection. The immune level of DNA vaccine was significantly improved by electric pulse method, and the antibody titer of 50 渭 g group could reach 1: 4 000. In addition, with the increase of CZP3 antibody level in serum, the infertility rate of mice increased obviously. Statistical analysis showed that the level of CZP3 antibody in immunized mice was negatively correlated with litter size. Conclusion: electric pulse method can significantly improve the immune level of CZP3 DNA vaccine and reduce the fertility of mice. This lays a foundation for the application of CZP3 DNA vaccine to contraception in stray dogs.
【作者單位】： 新疆大學(xué)生命科學(xué)與技術(shù)學(xué)院新疆生物資源基因工程重點(diǎn)實(shí)驗(yàn)室;
【基金】：新疆維吾爾自治區(qū)科技支疆項(xiàng)目(No.201591134)
【分類號(hào)】：S852.4;S829.2
，

本文編號(hào)：1544077