本文關(guān)鍵詞： 禽流感病毒 H亞型 N亞型 二重逆轉(zhuǎn)錄-聚合酶鏈反應(yīng)　出處：《動(dòng)物醫(yī)學(xué)進(jìn)展》2017年03期 　論文類型：期刊論文

【摘要】：為了建立一種快速、簡(jiǎn)便的H5亞型、N6亞型禽流感病毒(AIV)的檢測(cè)方法,根據(jù)GenBank中H5亞型、N6亞型AIV的HA和NA基因保守序列,分別設(shè)計(jì)了2對(duì)特異性引物,通過(guò)優(yōu)化條件,建立了H5亞型和N6亞型AIV二重RT-PCR檢測(cè)方法。特異性試驗(yàn)結(jié)果顯示,該方法對(duì)H5N6亞型AIV特異性擴(kuò)增出418bp和251bp目的片段,對(duì)H5Ny(y≠6)亞型AIV擴(kuò)增出418bp目的片段,對(duì)HxN6(x≠5)亞型AIV擴(kuò)增出251bp目的片段,對(duì)其他亞型和常見(jiàn)的禽病病原體均未擴(kuò)增出目的片段;敏感性結(jié)果顯示,該方法對(duì)H5亞型和N6亞型最低檢測(cè)限為1.59×10-5ng/μL。本研究建立的H5亞型和N6亞型AIV檢測(cè)方法,具有特異性強(qiáng),靈敏度高的特點(diǎn),為H5亞型和N6亞型AIV臨床檢測(cè)以及防控提供了有效方法。
[Abstract]:In order to establish a rapid and simple method for the detection of H5 subtype N6 avian influenza virus, two pairs of specific primers were designed according to the conserved sequences of HA and na genes of H5 subtype N6 subtype AIV in GenBank. A double RT-PCR method for the detection of H5 subtype and N6 subtype AIV was established. The results of specific test showed that 418 BP and 251 BP target fragments were amplified by this method for H 5N 6 subtype AIV, and 418 BP target fragment was amplified for H 5N 6 subtype AIV. For HxN6(x 鈮，

本文編號(hào)：1532406