本文關(guān)鍵詞： 抗菌肽Cec Md 衍生抗菌肽 枯草芽孢桿菌 抑菌活性 熱穩(wěn)定性 蛋白酶敏感性　出處：《黑龍江畜牧獸醫(yī)》2017年15期 　論文類(lèi)型：期刊論文

【摘要】：為了檢測(cè)在枯草芽孢桿菌中表達(dá)的Cec Md及其衍生抗菌肽的生物活性,研究采用微生物試驗(yàn)技術(shù),對(duì)從枯草芽孢桿菌中表達(dá)后純化出的抗菌肽Cec Md及其衍生抗菌肽CC31、CC34的抑菌效果、熱穩(wěn)定性、最佳抗菌活性的pH值、保存穩(wěn)定性以及蛋白酶敏感性進(jìn)行了測(cè)定。結(jié)果表明:CC31、CC34和Cec Md對(duì)大腸桿菌、雞沙門(mén)氏菌和停乳鏈球菌有抑制作用;對(duì)有益菌短乳桿菌、德氏乳桿菌、卷曲乳桿菌、雙歧桿菌、畢赤酵母SMD1168、畢赤酵母GS115均沒(méi)有抑制作用;3種抗菌肽對(duì)大腸桿菌、雞沙門(mén)氏菌和停乳鏈球菌的最小抑菌濃度(MIC)均低于25μmol/L,CC31和CC34對(duì)金黃色葡萄球菌的MIC為25μmol/L,低于Cec Md(100μmol/L);CC31和CC34對(duì)致病菌的最小殺菌濃度(MBC)均不超過(guò)50μmol/L,Cec Md對(duì)停乳鏈球菌的MBC為50μmol/L,對(duì)其他3種致病菌的MBC均在75μmol/L以上。CC31和CC34的熱穩(wěn)定性較好,Cec Md在沸水中30 min失去抑菌活性;CC31和CC34在pH值低于6時(shí)均活性較好,Cec Md最佳活性pH值僅在4~5之間;3種抗菌肽的最佳保存溫度均為-80℃;3種抗菌肽經(jīng)胰蛋白酶、胃蛋白酶、蛋白酶K作用后活性均有所降低,其中蛋白酶K對(duì)3種抗菌肽活性影響較弱。枯草芽孢桿菌中表達(dá)出的抗菌肽CC31和CC34的抑菌活性與理化特性等方面都好于Cec Md,其中CC31的生物活性最好。
[Abstract]:In order to detect the bioactivity of Cec Md and its derived antimicrobial peptides expressed in Bacillus subtilis, the antimicrobial peptide Cec Md and its derived antimicrobial peptide CC31C34 were purified from Bacillus subtilis by microbiological test technique. The thermal stability, pH value of the best antibacterial activity, preservation stability and protease sensitivity were determined. The results showed that: 1. CC31CC34 and Cec Md could inhibit Escherichia coli, Salmonella chickensi and Streptococcus acuminatum, while they could inhibit the beneficial bacteria Lactobacillus brevis. Lactobacillus de, Lactobacillus coreus, Bifidobacterium, Pichia pastoris SMD1168 and Pichia pastoris GS115 had no inhibitory effect on Escherichia coli. The minimum inhibitory concentrations (MIC) of Salmonella and Streptococcus staphylococcus were lower than 25 渭 mol / L CC31 and CC34, the MIC of Staphylococcus aureus was 25 渭 mol / L, and the minimum bactericidal concentration of Cec Md(100 渭 mol / L CC31 and CC34 were not more than 50 渭 mol / L LCec Md, respectively. The MBC of Streptococcus acuminatum was less than 50 渭 mol / L LCec Md. 50 渭 mol / L, the MBC of the other three pathogens were all above 75 渭 mol/L. CC31 and CC34 had better thermal stability. CecMd lost its antibacterial activity in boiling water for 30 min. CC31 and CC34 had better activity when pH was lower than 6:00. The optimum pH value of CEC Md was only between 4 and 5. The optimum preservation temperature of antimicrobial peptides was -80 鈩，

本文編號(hào)：1526550