本文關(guān)鍵詞： 高脂飼糧 肉品質(zhì) 蛋白質(zhì)組學(xué) 豬　出處：《畜牧獸醫(yī)學(xué)報》2016年10期 　論文類型：期刊論文

【摘要】：本試驗旨在研究高脂飼糧對生長育肥豬肉品質(zhì)和骨骼肌蛋白質(zhì)組的影響。選擇20頭平均體重為(73.28±0.57)kg的生長育肥豬,隨機分為對照組(對照飼糧,含10.0%玉米淀粉)和試驗組(高脂飼糧,含10.0%豬油),每個處理10個重復(fù),每個重復(fù)1頭豬,試驗期42d。研究飼糧脂肪含量對生長育肥豬生長性能、肉品質(zhì)和骨骼肌蛋白質(zhì)表達譜差異的影響。結(jié)果表明:高脂飼糧顯著提高了生長育肥豬的平均日增重(P0.05),并降低了料重比(P0.01)。骨骼肌pH_(45min)、pH_(24h)、肌內(nèi)脂肪含量、亮度、紅度、黃度、蒸煮損失、滴水損失和剪切力不受飼糧脂肪含量的影響(P0.05)。蛋白質(zhì)組學(xué)分析表明,飼喂高脂飼糧上調(diào)了生長育肥豬骨骼肌中鈣網(wǎng)蛋白、肌球蛋白重鏈、加帽蛋白、丙酮酸激酶、烯醇化酶、乳酸脫氫酶、磷酸丙糖異構(gòu)酶、腺苷酸激酶、載脂蛋白A和B、脂肪酸結(jié)合蛋白、熱休克蛋白27ku和70ku等蛋白質(zhì)的表達量,但下調(diào)了肌球蛋白輕鏈和磷酸葡萄糖變位酶蛋白質(zhì)的表達量。由此可見,采食高脂飼糧可顯著改善生長育肥豬的生長性能,并上調(diào)骨骼肌中與脂質(zhì)代謝、葡萄糖和能量代謝等生物學(xué)功能相關(guān)的蛋白質(zhì)表達量。
[Abstract]:The purpose of this study was to study the effect of high fat diet on the quality of growing and fattening pork and skeletal muscle proteome. Twenty growing fattening pigs with average body weight of 73.28 鹵0.57 kg were randomly divided into control group (control diet). The experiment group (containing 10.0% corn starch) and the experimental group (high fat diet, containing 10.0% lard, 10 replicates per treatment, 1 pig per repeat, for 42 days) were used to study the effect of dietary fat content on growth performance of growing and fattening pigs. The results showed that high fat diet significantly increased the average daily gain (ADG) of growing and fattening pigs, and decreased the feed / weight ratio (P0.01C). Cooking loss, drip loss and shear stress were not affected by dietary fat content. Proteomics analysis showed that high fat diet upregulated calcium reticin, myosin heavy chain, capsin, pyruvate kinase in skeletal muscle of growing and fattening pigs. The expression of enolase, lactate dehydrogenase, propanoisomerase, adenylate kinase, apolipoprotein A and B, fatty acid binding protein, heat shock protein 27 ku and 70 ku, etc. But the expression of myosin light chain and phosphoglucose transcriptase protein was down-regulated. It can be seen that feeding high fat diet can significantly improve the growth performance of growing and fattening pigs, and up-regulate the metabolism of lipid in skeletal muscle. The amount of protein expression related to biological functions such as glucose and energy metabolism.
【作者單位】： 西南科技大學(xué)生命科學(xué)與工程學(xué)院;中國農(nóng)業(yè)科學(xué)院北京畜牧獸醫(yī)研究所;
【基金】：動物營養(yǎng)學(xué)國家重點實驗室開放課題(2004DA125184F1424) 國家科技支撐計劃項目(2012BDA39B01) 中國農(nóng)業(yè)科學(xué)院科技創(chuàng)新工程(ASTIP-IAS07)
【分類號】：S828

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