本文關(guān)鍵詞： 偽狂犬病 抗體 陽性率 g E g B 血清學(xué)調(diào)查　出處：《黑龍江八一農(nóng)墾大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：豬偽狂犬病是由偽狂犬病毒引起的以繁殖障礙、腦脊髓炎、呼吸道疾病等癥狀為主的急性傳染病。目前黑龍江省偽狂犬病流行現(xiàn)狀呈上升趨勢,嚴重制約養(yǎng)豬業(yè)發(fā)展。本試驗通過血清學(xué)調(diào)查豬偽狂犬病抗體在規(guī)模化豬場的變化規(guī)律,同時開展不同毒株的三種疫苗在偽狂犬病陰性豬場和陽性豬場的免疫效果對比試驗,以期為豬場制定合理的免疫程序和選擇疫苗提供理論依據(jù)和試驗參考。本試驗對黑龍江省某存欄1 200頭母豬豬場在2015年9月-2016年10月分三次采集不同群體樣本血清進行豬偽狂犬病g E抗體及g B抗體檢測,同時針對斷奶前后不同日齡豬群進行豬偽狂犬病g B抗體檢測以確定母源及疫苗抗體的消減規(guī)律。結(jié)果顯示:2015年9月采集336頭豬群中g(shù) E抗體陽性豬208頭,陽性率61.90%,g B抗體陽性豬為314頭,陽性率為93.45%;2016年4月采集200頭豬群中g(shù) E抗體陽性豬120頭,陽性率60.00%,g B抗體陽性豬為174頭,陽性率為87.00%;2016年10月采集187頭豬群中g(shù) E抗體陽性豬為92頭,陽性率為49.20%,g B抗體陽性豬為141頭,陽性率為75.40%。其中2016年4月對豬場35日齡、45日齡、70日齡豬群檢測g B抗體陽性率分別為70.00%、50.00%、75.00%,45日齡仔豬g B抗體陽性率為50.00%,為適宜的首免日齡,結(jié)果顯示該豬場偽狂犬病感染情況較為嚴重,但野毒抗體呈下降趨勢。本次血清學(xué)調(diào)查結(jié)果為豬場開展偽狂犬病的防控,制定符合本場情況的免疫程序提供了依據(jù)。根據(jù)上述試驗結(jié)果選取豬偽狂犬病陰性場和陽性場進行三種不同疫苗免疫效果對比試驗,選取35日齡的60頭健康仔豬隨機分為3組分別免疫不同毒株的三種疫苗,分別為A疫苗(國產(chǎn)SA毒株)、B疫苗(國產(chǎn)Bartha毒株)、C疫苗(進口Bartha毒株),于首免前1天、二免前1天、二免28天后分別采集血清檢測豬偽狂犬病野毒g E抗體和疫苗g B抗體水平。結(jié)果顯示:陰性場豬偽狂犬病g E抗體陽性率為0%,說明豬場沒有受到野毒感染。二免前1天,A疫苗、C疫苗抗體陽性率在90%以上,B疫苗抗體陽性率為50%,二免后28天,A、C疫苗抗體陽性率為100%,高于B疫苗抗體陽性率90.00%。陽性豬場檢測結(jié)果顯示首免前1天g E、g B抗體陽性率差異不顯著(P0.05),二免前1天檢測豬偽狂犬g E抗體陽性率分別為50%、45%、40%,g B抗體陽性率分別為50%、60%、65%;二免后28天檢測豬偽狂犬g E抗體陽性率分別為35%、40%、35%,豬偽狂犬g B抗體分別為75%、70%、80%。本次疫苗對比試驗為豬場選擇符合本場的疫苗提供了數(shù)據(jù)依據(jù)。綜上所述,黑龍江某1 200頭規(guī)�；i場偽狂犬病野毒感染呈下降趨勢,根據(jù)母源抗體消減規(guī)律,最佳首免日齡為45日齡。對比疫苗試驗結(jié)果顯示三種疫苗均能在二免后產(chǎn)生有效抗體,首免后國產(chǎn)SA疫苗與進口Bartha疫苗免疫效果優(yōu)于國產(chǎn)Bartha疫苗,國產(chǎn)SA疫苗與進口Bartha疫苗差異不大。
[Abstract]:Porcine pseudorabies is an acute infectious disease caused by pseudorabies virus, which is characterized by reproductive disorders, encephalomyelitis, respiratory diseases, etc. At present, the prevalence of pseudorabies in Heilongjiang Province is on the rise. The changes of pseudorabies antibody in large scale pig farms were investigated by serology. At the same time, three kinds of vaccines of different strains were tested in the pseudorabies negative pig farm and the positive pig farm. The aim of this study was to provide theoretical basis and experimental reference for the establishment of reasonable immunization procedures and vaccine selection in pig farms. This experiment was used to collect blood samples from different populations three times from September 2015 to October 2016 in a pig farm in Heilongjiang province. The antibody against porcine pseudorabies virus (GE) and the antibody against porcine pseudorabies (GB) were detected. At the same time, porcine pseudorabies g B antibody was detected before and after weaning to determine the subtractive rule of maternal and vaccine antibodies. The results showed that: in September 2015, 208 pigs were positive for GE antibody. In April 2016, 120 of 200 pigs were positive for GE antibody, and the positive rate was 60.000.The positive rate was 60.00g B antibody positive pigs, and the positive rate was 93.455.In April 2016, the positive rate of g E antibody positive pigs was 60.00g B antibody positive pigs, and the positive rate was 93.455.In April 2016, the positive rate of g E antibody positive pigs was #number0# pigs. In October 2016, the positive rate of g E antibody was 92, and the positive rate was 49.20 g B antibody positive pigs. The positive rate of g B antibody was 75.40 in April 2016, and the positive rate of g B antibody in the piglets of 35 days old, 45 days old and 70 days old of pig farm was 70.0050.000.The positive rate of anti-G B antibody of 45 day old piglets was 50.000, which was the appropriate age of first day immunity, and the positive rate was 75.40g B antibody positive rate of 45 days old piglets. The results showed that the infection of pseudorabies in the pig farm was more serious, but the antibody of wild virus showed a downward trend. The serological investigation showed that the prevention and control of pseudorabies was carried out in the pig farm. According to the results of the above test, three different vaccine immunization efficacy comparative tests were carried out in pig pseudorabies negative field and positive field. Sixty healthy piglets of 35 days old were randomly divided into three groups: a vaccine (A vaccine) (domestic SA virus strain B vaccine) (domestic Bartha virus strain C vaccine) (imported Bartha virus strain), one day before the first immunization, and one day before the second immunization. After 28 days of immunization, serum samples were collected to detect the level of antibody against bovis pseudorabies virus E and vaccine GB respectively. The results showed that the positive rate of antibody against pseudorabies was 0, indicating that the pig farm had not been infected by wild virus. The positive rate of antibody against B vaccine was more than 90% on the first day of immunization, and the positive rate was 100 on the 28th day after the second immunization, which was higher than the positive rate of antibody against B vaccine. The positive rate of positive pig farm showed that the positive rate of antibody was 100% on the first day of immunization. The positive rate of positive pig farm showed that the positive rate was 100% on the first day of immunization. There was no significant difference in the positive rate of antibody against g EG B in pigs. The positive rates of antibody against bogus rabies in pigs were 50 ~ 45 and 40g / B respectively one day before the second immunity, and the positive rates of antibody were 50 ~ 6060 ~ 65g / B respectively, and the positive rates were 35404040 / 3535 and 35404040 / 3550 respectively on the 28th day after the second immunization, and the positive rates were 35404040 / 3550 for porcine pseudorabies g / E antibody, respectively. Rabies g B antibody was 750.The vaccine contrast test provided the data basis for the pig farm to select the vaccine according to this field. The infection of pseudorabies wild virus in a large scale pig farm in Heilongjiang showed a downward trend. According to the rule of maternal antibody subtractive, the best age of first immunization was 45 days old. The results of the vaccine test showed that all three vaccines could produce effective antibodies after the second immunization. The immunization effect of domestic SA vaccine and imported Bartha vaccine was better than that of domestic Bartha vaccine, but there was no significant difference between domestic SA vaccine and imported Bartha vaccine.
【學(xué)位授予單位】：黑龍江八一農(nóng)墾大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：S858.28

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