本文關(guān)鍵詞： 抗體消長 液相阻斷ELISA 免疫 非結(jié)構(gòu)蛋白3ABC　出處：《甘肅農(nóng)業(yè)大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：口蹄疫(Foot-and-Mouth Disease)是引起偶蹄動(dòng)物的熱性、急性、高度接觸性的傳染病,如果一旦發(fā)病其發(fā)病率可以到達(dá)100%,死亡率達(dá)到20%~50%,嚴(yán)重影響了全球畜牧業(yè)的健康發(fā)展。發(fā)達(dá)國家防控口蹄疫主要是采用全面捕殺感染動(dòng)物的方式,我國對口蹄疫的防控措施主要采用的是疫苗免疫的方式。牛是FMDV最易感動(dòng)物,尤其是奶牛,具有排毒量大的特點(diǎn)。羊患此病后臨床癥狀較其他偶蹄動(dòng)物相比并不明顯,故成為不易被發(fā)現(xiàn)的傳染源存在,而豬對FMDV非常敏感,患病豬可以產(chǎn)生大量氣溶性病毒,一頭病豬其氣源性排毒量相當(dāng)于1000~3000頭患病牛的釋放量,故檢測豬、牛、羊抗體消長變化規(guī)律及感染情況對FMD的防控具有極大的指示作用。3ABC是FMDV編碼的非結(jié)構(gòu)蛋白,3ABC抗體被用來作為鑒別診斷以區(qū)分感染和非感染動(dòng)物。本研究選取不同日齡豬群作為實(shí)驗(yàn)對象,嚴(yán)格按照免疫程序進(jìn)行免疫,在檢測抗體效價(jià)變化的同時(shí)重點(diǎn)研究口蹄疫疫苗免疫次數(shù)與3ABC陽性率關(guān)系,結(jié)果顯示在豬體內(nèi)免疫次數(shù)與3ABC陽性率之間并無明顯線性關(guān)系。針對牛群分別于第0天、第157天進(jìn)行進(jìn)行兩次免疫,免疫后分4個(gè)時(shí)間點(diǎn)采血并檢測O、A和Asia1型抗體的變化趨勢,結(jié)果表明使用實(shí)驗(yàn)組疫苗在首免及首免后157天免疫即可產(chǎn)生持續(xù)大于1:64滴度的抗體水平,能有效預(yù)防牛群口蹄疫的發(fā)生。針對羊群分別于第0天、第177天進(jìn)行進(jìn)行兩次免疫,免疫后分6個(gè)時(shí)間點(diǎn)采血并檢測三型抗體的變化趨勢,結(jié)果表面使用實(shí)驗(yàn)組疫苗在首免及首免后177免疫兩次即可產(chǎn)生大于持續(xù)1:64滴度的抗體水平,對羊群起到較好的保護(hù)作用。本研究結(jié)合生產(chǎn)實(shí)踐,對規(guī)模化豬場、牛場、羊場口蹄疫三價(jià)滅活疫苗抗體消長規(guī)律做了較為深入的研究,具有一定理論指導(dǎo)意義和實(shí)際應(yīng)用價(jià)值。
[Abstract]:Foot-and-mouth disease (Foot-and-Mouth disease) is a feverish, acute and highly contact infectious disease in cloven-hoofed animals. Once infected, it can reach 100%. The mortality rate has reached 20%, which has seriously affected the healthy development of animal husbandry in the world. The prevention and control of foot-and-mouth disease in developed countries mainly adopts the way of killing and killing infected animals. Vaccination is the main method of prevention and control of foot-and-mouth disease in China. Cattle are the most susceptible animals of FMDV, especially dairy cows. The clinical symptoms of sheep with this disease are not obvious compared with other cloven-hoofed animals, so it is difficult to find the source of infection, and pigs are very sensitive to FMDV. Infected pigs can produce a large number of air-soluble virus, a sick pig whose airborne excretion amount is equivalent to 1000 or 3000 sick cattle release, so the detection of pigs, cattle. The change rule of sheep antibody and infection condition have a great indicative effect on the prevention and control of FMD. 3ABC is a non-structural protein encoded by FMDV. 3ABC antibody was used as differential diagnosis to distinguish infected and non-infected animals. In this study, pigs of different ages were selected as experimental subjects and immunized strictly according to the immune procedure. The relationship between the number of times of immunization and the 3ABC positive rate of foot-and-mouth disease vaccine was studied at the same time as the change of antibody titer. The results showed that there was no significant linear relationship between the number of immunizations in pigs and the positive rate of 3ABC. The cows were immunized twice on day 0 and day 157 respectively. The blood samples were collected at four time points after immunization and the changes of OMA and Asia1 antibodies were detected. The results showed that the antibody level of above 1: 64 titers could be produced after the first immunization and 157 days after the first immunization, which could effectively prevent the occurrence of foot-and-mouth disease in cattle. Two immunizations were carried out on the 177th day. The blood samples were collected at 6 time points after immunization and the trend of type III antibody was detected. Results the antibody level was higher than 1: 64 titers after the first immunization and 177 immunization after the first immunization. The vaccine had a better protective effect on sheep. This study combined with production practice. The growth and decline rule of inactivated vaccine antibody against foot-and-mouth disease (FMD) in large scale pig farm, cattle farm and sheep farm were studied in depth, which has a certain theoretical significance and practical application value.
【學(xué)位授予單位】：甘肅農(nóng)業(yè)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：S852.4

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