本文關(guān)鍵詞： 馬泰勒蟲(chóng) 感染 系統(tǒng)發(fā)育　出處：《新疆農(nóng)業(yè)科學(xué)》2017年11期 　論文類(lèi)型：期刊論文

【摘要】：【目的】分析新疆南北疆馬泰勒蟲(chóng)感染的差異性及地方流行株遺傳進(jìn)化距離,研究馬泰勒蟲(chóng)遺傳多樣性及其感染率�！痉椒ā坎勺阅媳苯�478份疑似馬匹的血樣,經(jīng)馬泰勒蟲(chóng)PCR方法檢測(cè),分析馬泰勒蟲(chóng)感染情況并應(yīng)用最大似然法(ML)基于18 S rRNA基因的遺傳進(jìn)化樹(shù)及構(gòu)建系統(tǒng)發(fā)育樹(shù)。【結(jié)果】在所采集的樣品中,中國(guó)新疆北疆地區(qū)馬泰勒蟲(chóng)感染陽(yáng)性率為13.96%(25/179),中國(guó)新疆南疆地區(qū)馬泰勒蟲(chóng)感染陽(yáng)性率為27.09%(78/299)。印度、南非、西班牙、伊朗等地方株聚為一支,擴(kuò)增的18S rRNA基因(MF398476、MF398477)與瑞典地方株聚為一支。【結(jié)論】中國(guó)新疆南疆馬泰勒蟲(chóng)感染率高于北疆,南北疆馬泰勒蟲(chóng)感染存在顯著性差異(P=0.0010.05),在不同年齡段馬匹的感染無(wú)顯著性差異(P0.05);擴(kuò)增的馬泰勒蟲(chóng)阿勒泰、托克遜地方流行株(MF398477、MF398476)與瑞士地方株(KM046918.1)親緣關(guān)系最近。
[Abstract]:[objective] to analyze the difference of Maleylla infection and the genetic and evolutionary distance of endemic strains in north and south Xinjiang. To study the genetic diversity and infection rate of T. equinalis. [methods] 478 blood samples from northern and southern Xinjiang were collected and detected by PCR method. The genetic evolution tree based on the 18s rRNA gene and the phylogenetic tree were analyzed by using the maximum likelihood method (MLL). [results] the phylogenetic tree was constructed in the samples collected. The positive rate of MarTaylor's infection was 13.96% / 179% in northern Xinjiang, and 27.09% / 299% in southern Xinjiang, China. South Africa, Spain, Iran and other local strains clustered into one, amplified 18s rRNA gene MF398476. MF398477) and the local strains of Sweden were clustered into one branch. [conclusion] the infection rate of Maleylla in southern Xinjiang of China is higher than that in northern Xinjiang. There was a significant difference in infection between north and south Xinjiang (P0. 0010.05), but there was no significant difference in infection of horses in different age groups (P0. 05). The phylogenetic relationship between the amplified strains of Altay, Tocson endemic strain MF398477 and Swiss strain KM046918.1) was the most recent.
【作者單位】： 新疆農(nóng)業(yè)大學(xué)動(dòng)物醫(yī)學(xué)學(xué)院;和靜畜牧獸醫(yī)站;昭蘇縣畜牧獸醫(yī)站;
【基金】：國(guó)家自然科學(xué)基金項(xiàng)目-新疆聯(lián)合基金重點(diǎn)項(xiàng)目“新疆馬梨形蟲(chóng)病流行規(guī)律及其優(yōu)勢(shì)媒介蜱生物學(xué)特性和檢測(cè)技術(shù)的研究”(U1403283) 新疆農(nóng)業(yè)大學(xué)研究生科研創(chuàng)新項(xiàng)目課題“馬泰勒蟲(chóng)病中西藥物效果篩選及治療”(XJAUGRI2016-009)~~
【分類(lèi)號(hào)】：S858.21
【正文快照】： 0引言【研究意義】馬泰勒蟲(chóng)是由硬蜱傳播的頂復(fù)門(mén)血液內(nèi)寄生蟲(chóng),主要寄生于馬、騾、驢和斑馬等動(dòng)物的網(wǎng)狀內(nèi)皮系統(tǒng)及紅細(xì)胞內(nèi)[1]。引起馬臨床呈現(xiàn)稽留熱、貧血、出血、黃染、呼吸困難等癥狀[2-4],OIE將其列為B類(lèi)疫病,我國(guó)將其列為二類(lèi)疫病[5]�！厩叭搜芯窟M(jìn)展】馬泰勒蟲(chóng)病呈世

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