本文關(guān)鍵詞： 鴨源禽Ⅰ型副黏病毒 生物學(xué)特性 遺傳特性 油乳劑滅活疫苗　出處：《中國(guó)畜牧獸醫(yī)》2017年04期 　論文類型：期刊論文

【摘要】：試驗(yàn)旨在測(cè)定一株從番鴨中分離獲得的禽Ⅰ型副黏病毒(GSFY株)的生物學(xué)特性和遺傳特性,并將其制成油乳劑滅活疫苗,進(jìn)行番鴨免疫保護(hù)試驗(yàn)。結(jié)果顯示,GSFY株的平均致死時(shí)間(mean death time,MDT)為71h,腦內(nèi)接種致病指數(shù)(intracerebral pathogenicity index,ICPI)為1.75,靜脈接種致病指數(shù)(intravenous pathogenicity index,IVPI)為2.49;GSFY株F基因核苷酸序列同源性分析顯示,其與雞源Chicken/China/Guangxi 9/2003株同源性最高,為97.1%,且二者均處于基因Ⅶ型分支,綜合以上結(jié)果確定其為禽Ⅰ型副黏病毒基因Ⅶ型強(qiáng)毒株。免疫保護(hù)試驗(yàn)結(jié)果顯示,疫苗進(jìn)行3次免疫后對(duì)番鴨有良好的免疫保護(hù)效果,3次免疫后第7天抗體效價(jià)平均值達(dá)到6log2,第21天抗體效價(jià)平均值達(dá)到8log2,用GSFY毒株攻擊免疫番鴨,當(dāng)抗體滴度5log2時(shí),保護(hù)率達(dá)100%。本研究結(jié)果為生產(chǎn)實(shí)踐制訂鴨預(yù)防禽Ⅰ型副黏病毒病的免疫程序提供參考。
[Abstract]:The aim of the experiment was to determine the biological and genetic characteristics of a strain of avian paramyxovirus type I (GSFY) isolated from muscovy duck and to make it into an inactivated oil emulsion vaccine. The results showed that the mean death time and MDT of GSFY strain was 71 h. The intracerebral pathogenicity index (ICPI) was 1.75. The virulence index of intravenous pathogenicity was 2.49; The nucleotide sequence homology analysis of F gene of GSFY strain showed that it had the highest homology with chicken Chicken/China/Guangxi 9/2003 strain (97.1%). Both of them were in the branch of gene 鈪，

本文編號(hào)：1485344