本文關(guān)鍵詞： 過(guò)氧化物酶體增殖物激活受體γ(PPARγ) 豬 血管內(nèi)皮細(xì)胞　出處：《中國(guó)畜牧獸醫(yī)》2017年09期 　論文類型：期刊論文

【摘要】：試驗(yàn)旨在研究過(guò)氧化物酶體增殖物激活受體γ(peroxisome proliferator-activated receptorsγ,PPARγ)對(duì)豬血管內(nèi)皮細(xì)胞增殖、遷移及小管形成的影響,并探討PPARγ在豬體外血管生成中的作用。設(shè)置PPARγ激動(dòng)劑組(5、10、15、20μmol/L羅格列酮)、抑制劑組(5、10、15、20μmol/L T0070907)及對(duì)照組,通過(guò)iCelligence細(xì)胞功能分析、劃痕試驗(yàn)和Matrigel基質(zhì)膠三維培養(yǎng),構(gòu)建豬體外血管生成的模型,模擬豬體內(nèi)血管生成的環(huán)境,分別對(duì)豬血管內(nèi)皮細(xì)胞的增殖、遷移、小管形成能力進(jìn)行測(cè)定,同時(shí)根據(jù)NCBI已有的相關(guān)序列,應(yīng)用Primer Premier 5.0軟件設(shè)計(jì)PPARγ基因特異性引物,利用SYBR GreenⅠ實(shí)時(shí)熒光定量PCR檢測(cè)PPARγ基因mRNA相對(duì)表達(dá)量,對(duì)PPARγ的體外作用效果進(jìn)行驗(yàn)證。結(jié)果顯示,5、10μmol/L羅格列酮能促進(jìn)豬血管內(nèi)皮細(xì)胞增殖、遷移及小管形成,T0070907的抑制效果在試驗(yàn)濃度區(qū)間內(nèi)(5~15μmol/L)隨濃度升高而加強(qiáng),較高濃度(20μmol/L)的兩種藥物均由于藥物毒性的影響對(duì)細(xì)胞活動(dòng)產(chǎn)生干擾。此外,5~20μmol/L羅格列酮和5~20μmol/L T0070907能分別提高和降低PPARγ基因mRNA相對(duì)表達(dá)量,且濃度趨勢(shì)與增殖、遷移、小管形成的試驗(yàn)結(jié)果一致。綜上所述,通過(guò)激活PPARγ可以對(duì)豬血管內(nèi)皮細(xì)胞的增殖、遷移及小管形成產(chǎn)生促進(jìn)效果,提示其在豬體外血管生成中具有積極作用,可為研究PPARγ對(duì)豬胎盤血管發(fā)生的影響提供參考依據(jù)。
[Abstract]:The aim of this study was to study peroxisome proliferator-activated receptors 緯-peroxisome receptor. The effects of PPAR 緯 on the proliferation, migration and tubule formation of porcine vascular endothelial cells, and the role of PPAR 緯 in porcine angiogenesis in vitro were investigated. 20 渭 mol/L rosiglitazone and 1520 渭 mol/L T0070907 in the inhibitor group and the control group were analyzed by iCelligence cell function analysis. Scratch test and three-dimensional culture of Matrigel matrix glue were used to construct the model of porcine angiogenesis in vitro, to simulate the environment of pig angiogenesis, and to proliferate and migrate porcine vascular endothelial cells, respectively. The ability of tubule formation was measured and the specific primers of PPAR 緯 gene were designed by using Primer Premier 5.0 software according to the related sequences of NCBI. The relative expression of PPAR 緯 gene mRNA was detected by SYBR Green 鈪，

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