本文關(guān)鍵詞： 玉米DDGS 酶水解物能值 定標(biāo)樣品　出處：《動物營養(yǎng)學(xué)報》2017年04期 　論文類型：期刊論文

【摘要】：本試驗旨在探討玉米干酒糟及其可溶物(DDGS)有效能值估測模型中定標(biāo)樣品的選擇原則。從23個玉米DDGS樣品(定義為全樣品庫)中按酶水解物能值(EHGE)相差0.21 MJ/kg左右的梯度選擇9個定標(biāo)玉米DDGS樣品,定義為選擇性樣品庫;將剩余的14個玉米DDGS樣品定義為非選擇性樣品庫。然后,比較選擇性樣品庫與非選擇性樣品庫化學(xué)成分含量及變異的差異,以及通過全樣品庫和選擇性樣品庫分別建立其化學(xué)成分對EHGE之間的回歸模型,比較根據(jù)回歸模型計算得到的非選擇性樣品庫EHGE的差異。結(jié)果表明,選擇性樣品庫和非選擇性樣品庫的玉米DDGS在粗蛋白質(zhì)(CP)、粗灰分(Ash)、粗脂肪(EE)、粗纖維(CF)、中性洗滌纖維(NDF)、酸性洗滌纖維(ADF)含量及EHGE平均值上均無顯著性差異(P0.05),CP、Ash、EE、CF、ADF、NDF含量及EHGE變異的方差上均無顯著性差異(P0.05)。選擇性樣品庫和非選擇性樣品庫化學(xué)成分含量在第一、二主成分得分載荷分布上,選擇性樣品庫中僅1個玉米DDGS樣品未與非選擇性樣品庫的分布范圍重疊。以選擇性樣品庫樣品建立的EHGE預(yù)測模型為EHGE=(3 566+53.94×EE-32.68×NDF)×4.184/1 000(R2=0.798 1,RSD=0.43 MJ/kg);以全樣品庫樣品建立的預(yù)測模型為EHGE=(3 742+29.67×EE-29.71×NDF)×4.184/1 000(R2=0.535 0,RSD=0.44 MJ/kg)。由2個模型獲得的非選擇性樣品庫(n=14)玉米DDGS的EHGE計算值與其實測值的絕對殘差平均值分別為0.47和0.33 MJ/kg,差異不顯著(P0.05)。綜上所述,在玉米DDGS有效能值的估測建模中,以EHGE作為定標(biāo)樣品的選擇依據(jù)是可行的。
[Abstract]:The purpose of this experiment was to explore the selection principle of calibration samples in the model of estimating the effective value of corn dry distiller's grains and their soluble substance DDGs. 23 corn DDGS samples (defined as the whole sample bank) were selected. Nine standard corn DDGS samples were selected according to the gradient of enzyme hydrolysate energy value (EHGE) of 0.21 MJ/kg. Defined as a selective sample library; The remaining 14 maize DDGS samples were defined as non-selective sample banks. Then, the differences of chemical composition content and variation between selective sample banks and non-selective sample banks were compared. The regression models of the chemical components to EHGE were established by the whole sample library and the selective sample library, and the difference of the non-selective sample library EHGE calculated by the regression model was compared. DDGS in crude protein, crude ash, crude fat, crude fiber and neutral washing fiber were observed in both selective and non-selective sample banks. There was no significant difference in the content of ADF and the average value of EHGE in acid detergent fiber. There was no significant difference in NDF content and variance of EHGE variation (P0.05N). The contents of chemical components in the first and second principal component scores of the selective and non-selective sample banks were distributed in the first and second principal components. Only one maize DDGS sample in the selective sample bank did not overlap with the distribution range of the non-selective sample bank. The EHGE prediction model established by the selective sample bank was EHGEN (. 3 566 53.94 脳 EE-32.68 脳 NDF) 脳 4. 184 / 1 000 R2N 0. 798 1. RSD=0.43 MJ / kg; The prediction model of the whole sample bank is EHGE=(3 742 29.67 脳 EE-29.71 脳 NDF) 脳 4. 1844 / 1 000 R2O 0.535 0. RSD=0.44 MJ / kg. Non-selective sample library obtained from two models. The average absolute residuals between the calculated EHGE values and the measured values of maize DDGS were 0.47 and 0.33 MJ/kg, respectively. The difference was not significant (P 0.05). In conclusion, it was feasible to select EHGE as the calibration sample in estimating the effective value of maize DDGS.
【作者單位】： 中國農(nóng)業(yè)科學(xué)院北京畜牧獸醫(yī)研究所動物營養(yǎng)學(xué)國家重點實驗室;新希望六和股份有限公司;
【基金】：國家自然科學(xué)基金(30901037) 新希望六和股份有限公司合作項目(QGCHT1201403240001)
【分類號】：S816
【正文快照】： 在飼料可消化養(yǎng)分含量的估測模型中,定標(biāo)樣品的選擇直接影響估測模型的準(zhǔn)確性與估測精度[1]。因此,建立定標(biāo)樣品的選擇依據(jù)非常重要。目前,在飼料有效能值估測方程的建模中,人們通常隨機采集一定數(shù)量的樣品,在獲得了飼料原料的化學(xué)成分?jǐn)?shù)據(jù)集與有效能的實測數(shù)據(jù)后,通過逐步回

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