本文關(guān)鍵詞： 真胃左方變位 血液代謝譜 群體監(jiān)測 ROC曲線　出處：《吉林大學(xué)》2015年碩士論文　論文類型：學(xué)位論文

【摘要】：圍產(chǎn)期奶牛經(jīng)歷妊娠、分娩以及泌乳等一系列生理過程，尤其是高產(chǎn)奶牛在妊娠后期階段及泌乳初期階段發(fā)生生理性能量負(fù)平衡和先天免疫低下，，使得組織器官易發(fā)生機(jī)能和代謝紊亂。真胃左方變位就是最常見的奶牛圍產(chǎn)期疾病之一，是由多因素而引發(fā)的以真胃平滑肌弛緩為病理學(xué)基礎(chǔ)的真胃疾病，多發(fā)病于高產(chǎn)奶牛，且多于分娩后數(shù)日或1~2周內(nèi)發(fā)病。每年有大量奶牛由于發(fā)生真胃左方變位而導(dǎo)致產(chǎn)奶量下降，過早淘汰，甚至死亡，嚴(yán)重影響著我國奶牛業(yè)的發(fā)展。因此，開展對奶牛群體性監(jiān)測對該病的早期診斷和科學(xué)的預(yù)防尤為重要�！皠P普頓代謝譜”是通過監(jiān)測血液、乳液和尿液中的一些成分，來評(píng)價(jià)畜群或者個(gè)體的營養(yǎng)狀態(tài)，從而達(dá)到預(yù)警相關(guān)的營養(yǎng)代謝性疾病。本研究運(yùn)用預(yù)防醫(yī)學(xué)的觀點(diǎn)及理論，通過對奶牛血液譜的比較與分析，以期能夠預(yù)測、預(yù)報(bào)從而預(yù)防奶牛真胃左方變位以及其他生產(chǎn)疾病的發(fā)生，同時(shí)也可以對其飼養(yǎng)管理給予一定的指導(dǎo)。 本研究通過對健康奶牛和真胃左方變位奶牛，的基本特征、能量代謝狀態(tài)、肝功能及腎功能指標(biāo)、免疫學(xué)指標(biāo)以及礦物質(zhì)元素等指標(biāo)的測試，利用受試者工作特征曲線（ROC曲線）對相關(guān)指標(biāo)在奶牛真胃左方變位診斷中的效用進(jìn)行比較，以期發(fā)現(xiàn)可用于奶牛真胃左方變位預(yù)測、預(yù)報(bào)的指標(biāo)。結(jié)果表明，真胃左方變位奶牛存在能量負(fù)平衡引起的糖脂代謝障礙，肝臟功能和膽紅素代謝受到一定程度的影響，且胰島素敏感性降低。血鈣（Cut-point值為2.25mmol/L）可作為奶牛真胃左方變位監(jiān)測的首選指標(biāo)，INS、RQUICKI、NEFA、ALT、GGT、CREA、AST、ALT、Cl和K等可作為輔助指標(biāo)，并初步確定了其Cut-point值。真胃左方變位奶牛炎性反應(yīng)明顯，細(xì)胞因子IL-1、IL-2、IL-6、IL-8、IL-10、TNF-α顯著升高；急性期蛋白，如急性期蛋白補(bǔ)體C-1q、觸珠蛋白、血清淀粉樣蛋白A、血漿銅藍(lán)蛋白、α1-酸性糖蛋白和C-反應(yīng)蛋白等顯著升高。 總之，真胃左方變位奶牛存在能量負(fù)平衡和鈣負(fù)平衡，一定程度的肝臟功能異常和膽紅素代謝異常，胰島素敏感性降低和明顯的炎性反應(yīng)。奶牛真胃左方變位的監(jiān)測可采用多指標(biāo)聯(lián)合的方法。
[Abstract]:Perinatal cows experience a series of physiological processes, such as pregnancy, delivery and lactation, especially the physiological negative balance of energy and innate immunosuppression in the later stage of pregnancy and the early stage of lactation. The left transposition of the true stomach is one of the most common perinatal diseases in dairy cows, which is caused by many factors. It is more common in high-yielding cows than in the first few days or within 2 weeks after delivery. Every year a large number of dairy cows due to the occurrence of left transposition of the true stomach led to a decline in milk production premature elimination and even death. The development of dairy cattle industry in China is seriously affected. Therefore, it is very important to carry out the early diagnosis and scientific prevention of the disease by carrying out the group monitoring of dairy cows. "Capton metabolic spectrum" is through the monitoring of blood. Some components in the emulsion and urine to evaluate the nutritional status of livestock or individuals in order to achieve early warning of nutritional metabolic diseases. This study uses the viewpoint and theory of preventive medicine. By comparing and analyzing the blood spectrum of dairy cows, the authors hope to predict and forecast the occurrence of left abomasum displacement and other production diseases in dairy cows, and at the same time give some guidance to the raising and management of dairy cows. In this study, the basic characteristics, energy metabolism, liver function and renal function, immunological indexes and mineral elements of healthy cows and left abomasum cows were tested. Using the operating characteristic curve (ROC curve), the utility of the related indexes in the diagnosis of left abomasal displacement in dairy cattle was compared, in order to find out that it can be used to predict the left abomasal displacement in dairy cattle. The results showed that there were impaired glucose and lipid metabolism caused by negative energy balance, and liver function and bilirubin metabolism were affected to some extent. The serum calcium Cut-point value of 2.25 mmol / L could be used as the first choice for monitoring the left transposition of the true stomach. Nefa GGT creatine and K can be used as supplementary indexes. The Cut-point value was preliminarily determined. The inflammatory response was obvious in the left abomasum of dairy cattle, and the cytokine IL-1, IL-2U, IL-6, IL-8, IL-10. TNF- 偽 increased significantly. Acute phase proteins, such as complement C-1q, thioglobin, serum amyloid A, plasma ceruloplasmin, 偽 1-acidic glycoprotein and C-reactive protein, were significantly increased. In a word, there are negative balance of energy and calcium, abnormal liver function and abnormal metabolism of bilirubin. Low insulin sensitivity and obvious inflammatory response.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：S858.23

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 鐘榮珍;李建國;房義;賈文彬;吳志廣;;反芻動(dòng)物硒營養(yǎng)研究進(jìn)展[J];黃牛雜志;2005年06期

2 劉海霞;劉俊棟;殷潔鑫;李建基;;奶牛真胃變位的研究進(jìn)展[J];中國畜牧獸醫(yī);2011年11期

3 陳翔翔;;奶牛皺胃變位的發(fā)生及診治[J];養(yǎng)殖技術(shù)顧問;2011年08期

4 黃克和;;奶牛酮病和脂肪肝綜合癥研究進(jìn)展[J];中國乳業(yè);2008年06期

5 李延軍;李燕;許莉;李世杰;翁業(yè)斌;哈孜瑪;鄧江玲;;奶牛真胃變位的原因分析[J];中國乳業(yè);2010年11期

6 徐占云;秦睿玲;劉兵;;奶牛皺胃變位病因機(jī)理研究進(jìn)展[J];中國奶牛;2007年02期

7 齊長明,陳家璞,湯小朋;奶牛皺胃變位[J];中國獸醫(yī)雜志;1999年10期

8 祁生旺,劉俊平,麻生福;奶牛產(chǎn)后真胃變位治療方法比較[J];中國獸醫(yī)雜志;2002年10期

9 董強(qiáng);楊天耀;林德貴;楊必順;趙寶玉;;奶牛真胃左方變位的病因及發(fā)病機(jī)制[J];中國獸醫(yī)雜志;2005年12期

10 徐占云;秦睿玲;李繼連;王鵬;孫秀梅;;奶牛皺胃變位發(fā)病因素的調(diào)查[J];中國獸醫(yī)雜志;2008年03期

本文編號(hào)：1475069