本文關(guān)鍵詞： 菌草 靈芝菌絲 多糖 提取工藝 清除率　出處：《北方園藝》2017年09期 　論文類型：期刊論文

【摘要】：以鮮菌草與靈芝菌絲發(fā)酵得到的菌質(zhì)為試材,采用單因素試驗和正交實驗對其多糖進行提取,研究了不同的料液比、提取時間、提取溫度和提取次數(shù)對菌質(zhì)多糖提取率的影響,獲得最優(yōu)化的提取條件;用Fenton體系和鄰苯三酚自氧化體系研究了菌質(zhì)多糖對羥自由基和超氧自由基清除率的影響。結(jié)果表明:影響鮮菌草與靈芝菌絲發(fā)酵菌質(zhì)多糖提取得率的主次因素分別為提取溫度料液比提取次數(shù)提取時間;最佳提取工藝條件為料液比1∶30g·mL~(-1)、溫度100℃、提取時間4h、提取次數(shù)3次,以此最佳組合提取鮮菌草與靈芝菌絲發(fā)酵菌質(zhì)多糖時,其得率為4.88%。鮮菌草與靈芝菌絲發(fā)酵菌質(zhì)多糖對羥基自由基和超氧陰離子自由基有明顯的清除作用,并且隨著多糖濃度的增加,清除能力增強,表明鮮菌草與靈芝菌絲發(fā)酵菌質(zhì)多糖對羥基自由基和超氧陰離子自由基的清除能力與多糖濃度有明顯的量效關(guān)系。通過相關(guān)方程可以得到鮮菌草與靈芝菌絲發(fā)酵菌質(zhì)多糖清除羥基自由基的EC_(50)值為0.461mg·mL~(-1),清除超氧陰離子自由基的EC_(50)值為0.864mg·mL~(-1)。
[Abstract]:The polysaccharides from fresh fungus and Ganoderma lucidum were extracted by single factor experiment and orthogonal experiment. The different ratio of material to liquid and extraction time were studied. The effects of extraction temperature and extraction times on the extraction rate of polysaccharides were studied and the optimum extraction conditions were obtained. The effects of mycelia polysaccharides on the scavenging rates of hydroxyl radical and superoxide free radical were studied by Fenton system and pyrogallol autooxidation system. The main and secondary factors affecting the extraction rate of polysaccharides from fresh fungus and Ganoderma lucidum fermentation were the extraction time of extracting temperature ratio of material to liquid. The optimum extraction conditions were as follows: ratio of material to liquid 1: 30 g 路mL ~ (-1), temperature 100 鈩，

本文編號：1471216