本文關(guān)鍵詞： 附睪 褪黑素 褪黑素受體 基因表達 蛋白定位　出處：《內(nèi)蒙古農(nóng)業(yè)大學》2017年碩士論文　論文類型：學位論文

【摘要】：褪黑素(melatonin,Mel)對于動物生殖活動具有重要的調(diào)節(jié)作用,它通過褪黑素受體MT1、MT2發(fā)揮其功能。附睪是雄性動物的一個重要的生殖器官,是精子成熟和貯存的主要場所。本文研究了褪黑素受體在不同年齡綿羊附睪的表達和分布情況,為進一步研究褪黑素對附睪功能的調(diào)節(jié)提供基礎(chǔ)。研究結(jié)果如下:(1)通過實時熒光定量PCR檢測,MT1在不同年齡綿羊附睪的不同部位均有表達,對于幼齡羔羊(2～3月齡),MT1在附睪尾的轉(zhuǎn)錄水平顯著高于附睪頭和附睪體;青年綿羊(6～8月齡),MT1在附睪體和附睪尾的轉(zhuǎn)錄水平顯著高于附睪頭;成年綿羊(2～3歲),MT1在附睪尾的轉(zhuǎn)錄水平顯著高于附睪頭和附睪體。隨著年齡的增加,MT1在附睪部位的轉(zhuǎn)錄水平呈現(xiàn)降低的趨勢。MT2在不同年齡綿羊附睪的不同部位均有表達,其中幼齡羔羊,附睪頭MT2的轉(zhuǎn)錄水平顯著高于附睪體和附睪尾的;青年綿羊,MT2在附睪頭的轉(zhuǎn)錄水平極顯著高于附睪體和附睪尾,附睪尾的轉(zhuǎn)錄水平顯著高于附睪體;成年綿羊,MT2在附睪頭和附睪尾的轉(zhuǎn)錄水平極顯著高于附睪體。隨著年齡的增加,MT2在附睪部位的轉(zhuǎn)錄水平呈現(xiàn)升高的趨勢。(2)通過免疫組化技術(shù)對不同年齡綿羊附睪的不同部位的褪黑素受體蛋白分布進行分析,結(jié)果表明,MT1和MT2蛋白在各個年齡綿羊附睪的各個部位都有分布,且主要分布在附睪上皮細胞中,而且在青年綿羊和成年綿羊的附睪腔以及精子上也檢測到MT1和MT2蛋白的陽性信號�？傊�,在不同年齡綿羊附睪的不同部位都有MT1和MT2的存在,而且表達水平和分布狀態(tài)存在一定差異�？傮w上,隨著年齡的增加,綿羊附睪上MT1的表達呈下降趨勢,而MT2的表達呈升高趨勢。這也提示對于不同年齡綿羊,附睪中褪黑素可能通過MT1或/和MT2不同途徑發(fā)揮生理功能。
[Abstract]:Melatonin (melatonin) plays an important role in regulating animal reproductive activity through melatonin receptor MT1. The epididymis is an important reproductive organ of male animals and the main site for sperm maturation and storage. The expression and distribution of melatonin receptor in epididymis of sheep of different ages were studied. In order to provide a basis for further study on the regulation of melatonin on epididymal function. The results are as follows: 1) the expression of mt1 in different parts of epididymis of sheep of different ages was detected by real-time fluorescence quantitative PCR. The transcriptional level of MT1 in the tail of epididymis was significantly higher than that in the head of epididymis and the body of epididymis. The transcriptional level of MT1 in the epididymis and cauda epididymidis was significantly higher in young sheep than in the head of epididymis. The transcriptional level of MT1 in the caudal epididymis of adult sheep was significantly higher than that of the head of epididymis and the body of epididymis. The transcriptional level of MT1 in epididymis showed a decreasing trend. MT2 was expressed in different parts of epididymis of sheep of different ages, including young lambs. The transcription level of MT2 in the epididymal head was significantly higher than that in the epididymal body and cauda epididymidis. The transcriptional level of MT2 in the epididymal head was significantly higher than that in the epididymal body and cauda epididymidis, and the transcriptional level of the epididymal tail was significantly higher than that of the epididymal body. The transcriptional levels of MT2 in the head and tail of epididymis were significantly higher in adult sheep than in the body of epididymis. The distribution of melatonin receptor protein in different parts of epididymis of sheep of different ages was analyzed by immunohistochemistry. MT1 and MT2 proteins are distributed in all parts of epididymis of sheep of all ages, and are mainly distributed in epididymal epithelial cells. The positive signals of MT1 and MT2 protein were also detected in epididymal cavity and sperm of young sheep and adult sheep. In short, MT1 and MT2 were found in different parts of epididymis of sheep of different ages. On the whole, with the increase of age, the expression of MT1 in sheep epididymis showed a downward trend. However, the expression of MT2 increased, which suggested that melatonin in epididymis may play a physiological role through MT1 or / and MT2 in different age sheep.
【學位授予單位】：內(nèi)蒙古農(nóng)業(yè)大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：S826

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本文編號：1458771