本文關鍵詞： 梅花鹿 YWHAE基因 --蛋白 克隆 序列分析　出處：《農(nóng)業(yè)生物技術學報》2017年05期 　論文類型：期刊論文

【摘要】：YWHAEA(assignment of the human 14-3-3 epsilon isoform)基因編碼14-3-3ε蛋白,此蛋白在發(fā)育的生茸區(qū)骨膜中特異性高表達。為了克隆梅花鹿(Cervus nippon)YWHAE基因編碼區(qū)序列,預測其蛋白結(jié)構(gòu)和功能,并研究其在梅花鹿發(fā)育過程中的表達特性。本研究以梅花鹿生茸區(qū)骨膜為材料,采用RT-PCR技術克隆梅花鹿YWHAE基因編碼區(qū)序列,利用生物信息學手段預測其結(jié)構(gòu)與功能。實驗結(jié)果表明,梅花鹿YWHAE基因編碼區(qū)全長768 bp(Gen Bank No.KY797653),編碼255個氨基酸,氨基酸水平上與牛(Bos taurus)相似性高達99.6%;氨基酸序列分析表明,鹿YWHAE基因編碼的14-3-3蛋白相對分子量為29 163.86 D,主要定位在細胞質(zhì)內(nèi),屬于水溶性蛋白;預測鹿14-3-3蛋白具有19個磷酸化位點、不存在糖基化位點;該蛋白的二級結(jié)構(gòu)主要為螺旋結(jié)構(gòu),其三級結(jié)構(gòu)呈現(xiàn)出遞進螺旋狀態(tài)。預測14-3-3蛋白的結(jié)構(gòu)特點,本研究為探究14-3-3蛋白與其他蛋白的相互作用提供理論依據(jù),同時為進一步研究鹿茸發(fā)育的調(diào)控機理提供基礎依據(jù)。
[Abstract]:YWHAEA(assignment of the human 14-3-3 epsilon isoformin gene encodes 14-3-3 蔚 protein. In order to clone the coding region of Cervus nippon)YWHAE gene from sika deer, the protein structure and function were predicted. In this study, we used the periosteum of sika deer to clone the sequence of YWHAE gene coding region of sika deer by using RT-PCR technique. Bioinformatics was used to predict its structure and function. The results showed that the full-length YWHAE coding region of sika deer was 768 bp(Gen Bank no. KY797653). The amino acid encoding 255 amino acids is similar to Bos taurus at amino acid level. Amino acid sequence analysis showed that the relative molecular weight of 14-3-3 protein encoded by deer YWHAE gene was 29163.86D, which was mainly located in cytoplasm and belonged to water-soluble protein. It was predicted that deer 14-3-3 protein had 19 phosphorylation sites and no glycosylation sites. The secondary structure of the protein is mainly helical structure, and its tertiary structure shows a progressive helical state. The structural characteristics of 14-3-3 protein are predicted. This study provides a theoretical basis for exploring the interaction between 14-3-3 protein and other proteins, and provides a basis for further study on the regulation mechanism of velvet antler development.
【作者單位】： 中國農(nóng)業(yè)科學院特產(chǎn)研究所;
【基金】：中國梅花鹿基因組計劃(No.20140309016YY) 中國農(nóng)業(yè)科學院中央級公益性科研院所基本科研業(yè)務費專項(No.Y2016PT46和No.1610342016001) 吉林省科技發(fā)展計劃項目(No.20170307007YY)
【分類號】：S825
【正文快照】： 鹿茸是雄性鹿科動物的第二性征,是唯一可以完全再生的哺乳動物附屬器官(Fennessy,1984)。青春期,仔公鹿(Cervus nippon)額外脊上的生茸區(qū)骨膜(antlerogenic periosteum,AP)開始發(fā)育形成角柄和初角茸,此為公鹿生長出的第一茬茸(Hartwig,Schrudde,1974)。研究已經(jīng)證實,鹿額外脊，

本文編號：1455812