本文關(guān)鍵詞： 鴨 鉬 鎘 肝臟 凋亡基因　出處：《江西農(nóng)業(yè)大學(xué)》2015年碩士論文　論文類型：學(xué)位論文

【摘要】：本試驗(yàn)通過研究鉬鎘聯(lián)合誘導(dǎo)對麻鴨肝臟線粒體抗氧化功能、肝臟超微結(jié)構(gòu)及部分凋亡基因表達(dá)的影響,來探討鉬、鎘之間的毒理學(xué)關(guān)系。本試驗(yàn)以鉬酸銨[(NH4)6Mo7O24·4H2O]作為鉬源、以硫酸鎘(3CdSO4·8H2O)作為鎘源;將360羽11日齡“江南2號”麻鴨隨機(jī)分成6組(每組公母各30羽):各組在每千克基礎(chǔ)日糧中添加Mo、Cd的劑量分別為:I組:對照組(Mo 0mg,Cd 0mg)、II組:低鉬組(Mo 15mg,Cd 0mg)、III組:高鉬組(Mo 100mg,Cd 0mg)、IV組:鎘組(Mo 0mg,Cd 4mg)、V組:低鉬鎘組(Mo 15mg,Cd 4mg)、VI組:高鉬鎘組(Mo 100mg,Cd 4mg)。試驗(yàn)周期為120d,分別在試驗(yàn)的第30d、60d、90d、120d每組隨機(jī)選10羽鴨(公母各5羽)進(jìn)行剖殺并采集所需樣品;檢測肝臟線粒體MDA含量及CAT、NOS、SOD、XOD、T-AOC活性;檢測血清中TG、HDL-c、LDL-c、DBIL、IBIL、CP的含量;檢測肝臟凋亡基因Bcl-2、Bak-1和Caspase-3 mRNA的表達(dá)量及肝臟細(xì)胞超微結(jié)構(gòu)變化。試驗(yàn)結(jié)果如下:1.除低鉬組外,肝臟線粒體的XOD、CAT活性在整個試驗(yàn)均顯著低于對照組(P0.05);T-AOC、SOD活性在60d、90d、120d顯著低于對照組(P0.05);MDA、NOS含量顯著高于對照組(P0.05);且鉬鎘聯(lián)合組與單獨(dú)組比較差異顯著(P0.05)。2.除低鉬組外,血清TG、LDL-c、DBIL、IBIL的含量較對照組呈顯著上升的趨勢(P0.05);HDL-c、CP較對照組呈顯著下降趨勢(P0.05);且鉬鎘聯(lián)合組與單獨(dú)組比較差異顯著(P0.05)。3.除低鉬組外,肝臟Bak-1、Caspase-3基因表達(dá)量較對照組呈顯著上升趨勢(P0.05);試驗(yàn)組Bcl-2基因表達(dá)量較對照組呈顯著下降趨勢(P0.05),且鉬鎘聯(lián)合組與單獨(dú)組比較差異顯著(P0.05)。4.第120d的鎘組、高鉬組和高鉬鎘組的肝臟細(xì)胞超微結(jié)構(gòu)出現(xiàn)了核固縮、線粒體腫脹、空泡化以及嵴斷裂等病理變化。結(jié)論:鉬、鎘及其聯(lián)合誘導(dǎo)均會導(dǎo)致麻鴨肝臟線粒體發(fā)生抗氧化功能損傷;并且導(dǎo)致肝臟超微結(jié)構(gòu)出現(xiàn)核固縮、線粒體腫脹、空泡化以及嵴斷裂等病理變化;還導(dǎo)致了肝臟中Bak-1mRNA、capase-3mRNA表達(dá)上升和Bcl-2 mRNA表達(dá)下降,并且整體鉬鎘呈協(xié)同作用。
[Abstract]:In this study, the toxicological relationship between molybdenum and cadmium was studied by studying the effects of molybdenum and cadmium combined induction on antioxidant function, liver ultrastructure and partial apoptotic gene expression in liver mitochondria of Ma duck. In this experiment, ammonium molybdate was used to study the toxicological relationship between molybdenum and cadmium. [NH _ 4O _ 6Mo _ 7O _ 24 路4H _ 2O] was used as molybdenum source, and cadmium sulfate was used as CD _ 2O _ 3CdSO _ 4 路8H _ 2O source. 360 11-day-old "Jiangnan No.2" ducks were randomly divided into 6 groups (30 male and female in each group): Mo was added to the basal diet per kilogram in each group. The dosages of CD were as follows: group I: control group: control group: Mo 0 mg / g CD 0 mg / g; group II: low molybdenum group: Mo 15 mg / g CD 0 mg). III group: high molybdenum group, Mo 100 mg / CD, CD 0 mg, group IV, cadmium group, Mo 0 mg, CD 4 mg / L, group V, low molybdenum and cadmium group, Mo 15 mg. CD 4 mg / L group VI: high molybdenum and cadmium group, Mo 100 mg / L CD 4 mg / L. The test period was 120 days, and the test period was 90 days on the 30th day of the experiment. 10 ducks (5 male and 5 female) were randomly selected for 120 days and the samples were collected. The content of MDA in liver mitochondria and the activity of XODT-AOC were measured. To detect the content of BILCP in serum of TGN HDL-cr. The expression of apoptotic genes Bcl-2, Caspase-3 mRNA and ultrastructure of liver cells were detected. The results were as follows: 1. Except for low molybdenum group. The activity of XODcat in liver mitochondria was significantly lower than that in control group (P 0.05). The activity of SOD in T-AOC was significantly lower than that in the control group (P 0.05) at 60d ~ 90d ~ 120d. The content of nitric oxide synthase (NOS) in MDAN was significantly higher than that in control group (P 0.05). In addition to the low molybdenum group, the serum TGN LDL-cn DBIL was significantly different between the molybdenum and cadmium combined group and the single group. Compared with the control group, the content of IBIL increased significantly (P 0.05). Compared with the control group, HDL-cn CP decreased significantly (P 0.05). The difference between the molybdenum and cadmium combined group and the single group was significant (P 0.05). In addition to the low molybdenum group, liver Bak-1 was significantly different. Compared with the control group, the expression of Caspase-3 gene increased significantly (P 0.05). Compared with the control group, the expression of Bcl-2 gene in the trial group was significantly decreased, and the difference between the molybdenum and cadmium combination group and the single group was significant (P0.05. 4). The cadmium group at the 120th day showed significant difference. The ultrastructure of liver cells in high molybdenum group and high molybdenum cadmium group showed nuclear shrinkage, mitochondria swelling, vacuolation and cristal rupture. Cadmium and its combined induction could lead to the damage of antioxidant function in liver mitochondria of duck. The pathological changes of liver ultrastructure such as pyknosis, swelling of mitochondria, vacuolation and cristal fracture were also found. The expression of Bak-1 mRNA-capase-3 mRNA and the expression of Bcl-2 mRNA in liver were increased, and the whole molybdenum and cadmium showed synergistic effect.
【學(xué)位授予單位】：江西農(nóng)業(yè)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：S859.8

【相似文獻(xiàn)】

相關(guān)期刊論文 前4條

1 蘇榮勝;劉好朋;胡京京;潘家強(qiáng);韓前彪;胡鍇;李海琴;唐兆新;;銅孵育對雞肝臟線粒體呼吸功能的影響[J];中國獸醫(yī)學(xué)報(bào);2011年10期

2 石冬梅;王巖;向瑞平;皇甫和平;張華;唐兆新;徐家華;王友令;張勝;;內(nèi)毒素對肉雞肝臟線粒體復(fù)活體Ⅰ活性的影響及氨基胍的保護(hù)效應(yīng)[J];中國畜牧獸醫(yī);2013年01期

3 陳娟;孔一力;王曼;馬海田;;丙酮酸、丙酮酸肌酸和肌酸對肉雞肝臟線粒體蛋白/酶表達(dá)譜的影響[J];畜牧與獸醫(yī);2012年S1期

4 ;[J];;年期

相關(guān)會議論文 前3條

1 孫鍇;潘小海;黃詩穎;葉一舟;潘家祜;;銀杏酸對大鼠肝臟線粒體的損傷作用[A];全國第十一屆生化與分子藥理學(xué)學(xué)術(shù)會議論文集[C];2009年

2 陳娜;魯長武;熊燕;;非對稱性二甲基精氨酸在糖尿病大鼠肝臟線粒體功能障礙中的作用[A];第九屆全國心血管藥理學(xué)術(shù)會議論文集[C];2007年

3 陳娟;孔一力;王曼;馬海田;;丙酮酸、丙酮酸肌酸和肌酸對肉雞肝臟線粒體蛋白/酶表達(dá)譜的影響[A];全國動物生理生化第十二次學(xué)術(shù)交流會論文摘要匯編[C];2012年

相關(guān)重要報(bào)紙文章 前1條

1 王飛;古藺肝蘇的抗氧化作用[N];中國中醫(yī)藥報(bào);2007年

相關(guān)碩士學(xué)位論文 前4條

1 孫鍇;大鼠肝臟線粒體的體外功能評價及對其損傷與保護(hù)的研究[D];復(fù)旦大學(xué);2008年

2 王穎;中緬樹，

本文編號：1444036