發(fā)布時間：2018-01-19 01:20

  本文關(guān)鍵詞： 中藥 DVD 雞柔嫩艾美爾球蟲 抗球蟲指數(shù)　出處：《河南科技大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：雞球蟲病是養(yǎng)殖生產(chǎn)過程中造成嚴(yán)重?fù)p失的疾病之一。目前臨床上治療球蟲仍以化學(xué)抗球蟲藥為主,其使用廣泛,尤其是常作為飼料添加劑。由于球蟲類型以及生殖階段(有性生殖階段、無性生殖階段和孢子生殖階段)的特殊性,造成了在球蟲病的防治中要根據(jù)不同的階段采用不同的藥物,臨床上根據(jù)病情發(fā)展經(jīng)常選擇兩種或兩種以上的藥物聯(lián)合用藥才能達(dá)到較好的治療效果。隨著臨床對化學(xué)藥物的使用次數(shù)和劑量的增加,球蟲的耐藥性逐漸增強(qiáng),而且化藥的使用對動物機(jī)體造成的傷害很大,特別是磺胺類藥物的大劑量使用不但有腎毒性而且還有免疫抑制作用。中草藥無耐藥性、無不良反應(yīng)、無免疫抑制、無休藥期、無毒副作用,而且對球蟲病也有很好的預(yù)防和治療效果,因此加強(qiáng)研究中草藥對球蟲病的防治意義更加深遠(yuǎn)。為此,本研究在傳統(tǒng)中藥組方雞球蟲散的基礎(chǔ)上,為了增加殺蟲效果,去掉何首烏,加入常山,構(gòu)成新的抗球蟲中藥組方,并考察該組方與DVD聯(lián)合使用對球蟲的治療效果,以解決中藥起效慢、治療時間長的問題,又能減少化學(xué)藥物的使用,一定程度也能降低用藥成本,進(jìn)而更好的起到治療球蟲病的目的。本研究具體內(nèi)容如下:1.篩選最佳抗雞柔嫩艾美爾球蟲中藥組方比例試驗(yàn)為了篩選中藥抗球蟲組方(青蒿、常山、仙鶴草、白頭翁、肉桂)中青蒿與常山的最佳比例,制備了3個擬定組方(青蒿與常山比例分別為1:0.5、1:1、1:2),通過3個組方對人工感染雞柔嫩艾美爾球蟲病,以20g/kg飼料的劑量,進(jìn)行治療試驗(yàn),考察3個組方對人工感染雞球蟲病的治療效果。其實(shí)驗(yàn)數(shù)據(jù)為:存活率分別為:83%、90%、87%;相對增重率分別為:86.77%、94.43%、92.12%;抗球蟲指數(shù)分別為:163.77、179.83、174.72。結(jié)果果表明:3個組方對雞艾美爾球蟲病均有明顯的治療作用,其中組方2(青蒿與常山比例為1:1)的作用效果最好。故采用該組方進(jìn)行后續(xù)研究。2.篩選新中藥組方與DVD聯(lián)合用藥最佳劑量為篩選出抗球蟲中藥組方(青蒿、常山、仙鶴草、白頭翁、肉桂)與抗菌增效劑DVD(40 ppm))聯(lián)合使用對人工感染雞柔嫩艾美爾球蟲球蟲病的治療效果的最佳劑量,將3種不同濃度組方中藥:分別為第1組:10 g/kg飼料的低劑量中藥組、第2組:20g/kg飼料的中劑量中藥組、第3組:30 g/kg飼料的高劑量中藥組,與DVD(40PPM)聯(lián)合使用、第4組:不用中藥只用DVD(80 ppm)組、第5組空白對照組、第6組攻毒對照組,在飼料中混合均勻,感染球蟲的同時給藥。試驗(yàn)結(jié)果前4組的其存活率分別為:93%、97%、97%、83%;其盲腸病變值分別為:4.8、4.5、4.3、5.2;卵囊值分別為:1、0、0、1;抗球蟲指數(shù)分別為:173.8、181.77、187.79、160.83,第2組中劑量和第3組高劑量中藥組均能達(dá)到高效抗球蟲作用。從經(jīng)濟(jì)效益考慮以第2組中劑量中藥組(20 g/kg)飼料與DVD(40 ppm/d)聯(lián)合應(yīng)用做為新中藥組方進(jìn)行后續(xù)研究。3.新中藥組方與DVD聯(lián)合用藥防治雞球蟲病的臨床試驗(yàn)為確定抗球蟲新中藥組方與抗菌增效劑DVD聯(lián)合使用對自然感染雞柔嫩艾美爾球蟲球蟲病的治療效果,按推薦劑量把兩者聯(lián)合使用,在飼料中混合均勻,藥物對照組使用雞球蟲散進(jìn)行治療,給藥后第5 d,供試藥物組和藥物對照組對雞球蟲卵囊減少率分別為99.12%和98.80%,雞只存活率比藥物對照組高0.13%,料肉比與藥物對照組相比降低0.17。結(jié)果證明藥物以20 g/kg飼料劑量與DVD(40ppm/d)聯(lián)合拌料,連用5 d,對雞球蟲病的治療效果顯著高于傳統(tǒng)的雞球蟲散。研究結(jié)果表明:青蒿與常山新型抗球蟲組方的最佳比例(1:1)對雞艾美爾球蟲病具有良好的治療作用,其對實(shí)驗(yàn)雞的保護(hù)率達(dá)90%,ACI為179.83。青蒿與常山抗球蟲組方(1:1)與DVD(40 ppm/d)聯(lián)合用藥的最佳劑量為20g/kg飼料,連續(xù)使用5 d,對人工感染和自然感染柔嫩艾美爾球蟲病雞有良好的治療效果。
[Abstract]:Chicken coccidiosis is one of the serious loss of aquaculture production in the process of disease. The clinical treatment of coccidiosis is mainly chemical anticoccidial drugs, it is widely used, especially used as feed additives. Because of coccidia types and reproductive stages (stages of sexual reproduction, asexual stage and spore stage) particularity caused to according to the different stages of the different drugs in the prevention and treatment of coccidiosis, according to the clinical progression of the disease often choose two or more than two kinds of drugs in order to achieve a better therapeutic effect. With the increasing use of chemical drugs in the clinical dose and frequency of the coccidial drug resistance increases, and the use of drugs to cause the animal body hurts a lot, especially large doses of sulfonamides use not only has renal toxicity and immunosuppressive effect of Chinese herbal medicine. No drug resistance, no No adverse reaction, immune suppression, no withdrawal period, no toxic side effect, but also has a very good prevention and treatment of coccidiosis, so the significance of Chinese herbal medicine to strengthen the prevention and control of coccidiosis is more profound. Therefore, this study based on the traditional Chinese medicine prescription of Eimeria powder, in order to increase the insecticidal effect, remove Polygonum multiflorum join, Changshan, constitute a new anticoccidial prescription of traditional Chinese medicine, and the effects of the prescription and DVD combination on coccidiosis treatment effect, in order to solve the slow onset of traditional Chinese medicine, treatment time is long, but also can reduce the use of chemical drugs, to a certain extent can reduce the drug cost, and better play to the treatment of coccidiosis specific contents of this study are as follows: 1. the selection of the best anti chicken Eimeria tenella in order to test the proportion of prescription of traditional Chinese medicine screening of traditional Chinese medicine prescription anticoccidiosis (Artemisia annua, Changshan, agrimony, Pulsatilla, cinnamon) in Artemisia annua and Changshan The best proportion of preparation of 3 prescription (Artemisia and Changshan to respectively 1:0.5,1:1,1:2), through 3 prescriptions on artificial infection of Eimeria tenella in chickens, to feed 20g/kg dose, treatment test, to investigate the therapeutic effect of 3 prescriptions on artificial infected chicken coccidiosis. The experimental data: the survival rate: 83%, 90%, 87%; the relative growth rates were: 86.77%, 94.43%, 92.12%; anticoccidiosis index were: 163.77179.83174.72. result showed that 3 components on the obvious curative effect has the chicken Eimeria coccidiosis, prescription 2 (Artemisia annua and Changshan 1:1 ratio) the best effect. So the use of the prescription the follow-up study of.2. screening for screening new anticoccidial prescription of traditional Chinese medicine prescription of traditional Chinese medicine combined with DVD dosage (Artemisia annua, Changshan, agrimony, Pulsatilla, cinnamon) and antibacterial synergist DVD (40 ppm) combined with the use of artificial sense) The optimal dose of chicken Eimeria tenella coccidiosis infection treatment effect, the concentration of 3 different herbs respectively into first groups: low dose group, 10 g/kg feed second groups: middle dose traditional Chinese medicine group, 20g/kg feed third groups: high dose group 30 g/kg feed, DVD (40PPM) combined with use of fourth groups: do not use the traditional Chinese medicine with DVD (80 ppm) group, fifth groups of blank control group, sixth groups of controlgroup, uniformly mixed in the feed, while administration of coccidian infection. The test results of 4 groups before the survival rate were 93%, 97%, 97%, 83%; the cecal lesions values were: 4.8,4.5,4.3,5.2 = 1,0,0,1; oocysts; anticoccidiosis index were: 173.8181.77187.79160.83, third groups of high dose and dose of Chinese medicine group in the second groups were able to achieve efficient anticoccidial effect. From the economic consideration to the pharmaceutical dose in the second group (20 g/kg) in feed with DVD (40 ppm/d) combined with The application for clinical trials of new prescription for subsequent research on.3. new prescription of traditional Chinese medicine combined with DVD coccidiosis in chickens to determine the new anticoccidial prescription of traditional Chinese medicine combined with natural infection of Eimeria tenella coccidiosis treatment effect of antibacterial synergist DVD, according to the recommended dose to the combination, uniformly mixed in the feed, drugs the control group using chicken coccidia powder treatment, fifth d after administration, the drug group and drug control group reduced rates were 99.12% and 98.80% of chicken coccidian oocysts in chickens, the survival rate is 0.13% higher than the drug control group, the ratio of feed and the drug control group decreased compared with 0.17. results in a 20 g/kg dose of feed with DVD (40ppm/d) combined with mixing, for 5 d, the chicken coccidiosis treatment effect was significantly higher than that of the traditional chicken coccidia powder. The results showed that the optimal ratio of Artemisia annua and Changshan new anticoccidial prescription Cases (1:1) has good therapeutic effect on chicken Eimeria coccidiosis, the experimental chicken protection rate of 90%, ACI 179.83. and Changshan Artemisia anticoccidiosis group (1:1) and DVD (40 ppm/d) the best dosage combination for 20g/kg feed, continuous use of 5 d treatment has a good effect on Eimeria chicken coccidiosis artificial infection and natural infection.

【學(xué)位授予單位】：河南科技大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：S858.31

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 馬琰;馬慶濤;承南;李金貴;;青蒿素與抗菌增效劑聯(lián)合抗雞球蟲效果評價[J];中國家禽;2016年02期

2 宋萍;蘇洋洋;馬慶濤;李金貴;;青蒿素與柳氮磺吡啶對柔嫩艾美爾球蟲感染的療效觀察[J];中國獸醫(yī)學(xué)報;2014年08期

3 王敏;王麗萍;韓天龍;黃敬海;李國華;李志明;;雞常用抗球蟲藥的應(yīng)用[J];中國獸醫(yī)雜志;2014年06期

4 莫平華;馬慶濤;紀(jì)小霞;宋萍;陶建平;李金貴;;青蒿素對雞柔嫩艾美耳球蟲第二代裂殖子微線基因mRNA轉(zhuǎn)錄及雞盲腸組織結(jié)構(gòu)的影響[J];畜牧獸醫(yī)學(xué)報;2014年05期

5 宋萍;馬慶濤;蘇洋洋;紀(jì)小霞;李金貴;;青蒿素類藥物對雞柔嫩艾美耳球蟲感染的防治試驗(yàn)[J];中國家禽;2014年05期

6 郭志廷;梁劍平;韋旭斌;尚若鋒;郭文柱;王學(xué)紅;郝寶成;;常山提取物對人工感染雞柔嫩艾美耳球蟲病療效的觀察[J];中國獸醫(yī)學(xué)報;2013年07期

7 喻婉瑩;闞偉娟;于鵬霞;李敏敏;宋吉帥;趙烽;;青蒿素和二氫青蒿素的抗炎作用及機(jī)制[J];中國中藥雜志;2012年17期

8 劉偉娟;王鈺;尚迅;趙興濤;翟強(qiáng);;抗球蟲中藥制劑對肉雞胴體品質(zhì)的影響[J];中國畜禽種業(yè);2011年11期

9 郭紅斌;弓素梅;;青蒿素與青蒿水提液對感染柔嫩艾美耳球蟲肉雞的療效評價[J];動物醫(yī)學(xué)進(jìn)展;2011年03期

10 劉陸軍;莊曉薇;郭成亮;王小偉;;鹽霉素與中藥聯(lián)合用藥抗雞球蟲病的療效試驗(yàn)[J];湖北畜牧獸醫(yī);2010年09期

相關(guān)重要報紙文章 前1條

1 ;抗球蟲劑——抗硫胺素類[N];中國畜牧獸醫(yī)報;2009年

相關(guān)碩士學(xué)位論文 前3條

1 蘇士佳;二甲氧芐啶對Wistar大鼠的毒理學(xué)研究[D];華中農(nóng)業(yè)大學(xué);2011年

2 吳金鳳;安徽三個地區(qū)柔嫩艾美耳球蟲耐藥性檢測的研究[D];安徽農(nóng)業(yè)大學(xué);2009年

3 萬恩懷;馬杜霉素對肉仔雞免疫功能的影響研究[D];四川農(nóng)業(yè)大學(xué);2006年

，

本文編號：1442017