豬瘟與豬藍耳病疫苗不同免疫程序?qū)γ庖咝Ч绊懙难芯?/H1>
發(fā)布時間：2018-01-08 08:06
本文關(guān)鍵詞：豬瘟與豬藍耳病疫苗不同免疫程序?qū)γ庖咝Ч绊懙难芯?/strong> 出處：《沈陽農(nóng)業(yè)大學》2016年碩士論文 論文類型：學位論文
更多相關(guān)文章： 豬瘟 豬藍耳病 血液指標 體液免疫 細胞免疫
【摘要】：古典型豬瘟(CSF)是一種非常嚴重的豬病毒性傳染病,尤其會對人口較為稠密的以養(yǎng)豬為主的國家造成重大的經(jīng)濟損失。豬藍耳病(PRRS)是迄今為止全世界各地區(qū)范圍內(nèi)在經(jīng)濟方面上造成最大負面影響的豬傳染病之一,據(jù)美國相關(guān)部門統(tǒng)計,豬藍耳病每年對其本土養(yǎng)豬業(yè)保守估計造成超過約60億美元的經(jīng)濟財產(chǎn)損失。當機體感染這兩種疾病以后,最終都會由于免疫功能徹底喪失、繼發(fā)其他疫病混合感染而死。豬瘟和豬藍耳病由于其各自特殊的致病機理而同屬于免疫抑制類疾病。免疫抑制疾病的發(fā)生是造成各大養(yǎng)豬場免疫防控失敗最不容忽視的因素之一,因此要想做好各類豬病的有效防控,控制好免疫抑制疾病的發(fā)生才是當務之急。而當前對于豬瘟和豬藍耳病這兩種免疫抑制疾病尚未形成統(tǒng)一、良好的免疫程序。因此針對二者摸索出一套合理、有效的免疫程序刻不容緩。本試驗選取42頭健康未免疫的斷奶仔豬,將其中36頭隨機分為3組(A、B、C),每組設2個重復,另外6頭作為空白對照。A組先免疫豬瘟間隔7天后免疫豬藍耳病,B組反之,C組采取同時分點免疫。分別于免疫前以及免疫后的第10、20、30、40、50、60、70天進行前腔靜脈無菌采血,分別從血液指標、料重比、體液免疫中特異性抗體的分泌水平、細胞免疫中T淋巴細胞的增殖能力以及IFN-γ的分泌水平這五個方面進行比較分析,結(jié)果如下：①血液指標結(jié)果顯示A組和C組平均白細胞數(shù)和淋巴細胞比例分別極顯著高于B組(p0.01)和對照組(p0.01),而其他指標各組間均無顯著差異(p0.05)。②料重比結(jié)果A組最低,C組其次,B組最高。③特異性抗體的ELISA試劑盒檢測結(jié)果顯示A組豬瘟抗體平均阻斷率在免疫后第70天左右達到峰值并極顯著高于B組(p0.01)、C組(p0.01)和對照組(p0.01),實驗組中B組最低(p0.01)；三個實驗組豬藍耳病抗體平均S/P值均呈上升趨勢,在免疫后第40天左右達到峰值,之后走勢趨于平穩(wěn),實驗組免疫前后差異均極顯著(p0.01),但組間差異不顯著(p0.05),對照組無趨勢變化。④T淋巴細胞增值能力結(jié)果顯示A組平均刺激指數(shù)在免疫后第40天左右達到峰值并極顯著高于B組(p0.01)、C組(p0.01)和對照組(p0.01),實驗組中B組最低(p0.01)。⑤IFN-γ分泌水平結(jié)果顯示A組平均IFN-γ濃度在免疫后第30天左右達到峰值并極顯著高于B組(p0.01)和對照組(p0.01),與C組差異不顯著(p0.05)綜上所述,不論是從血液指標,還是從料重比、體液免疫水平以及細胞免疫水平上來看,均可以得出一致結(jié)論,即先免疫豬瘟,間隔7天后再免疫豬藍耳病的免疫效果較好,此結(jié)論為將來制定豬瘟、豬藍耳病合理有效的免疫程序提供了一定的理論依據(jù)。
[Abstract]:Classical swine fever (CSF) is a very serious swine viral disease. In particular, significant economic losses will be caused to more densely populated countries where pig breeding is the main source of disease. Pig Blue ear Disease (PRRSs). So far, it is one of the most economically negative swine infectious diseases in all regions of the world. According to relevant departments in the United States, blue ear disease causes an estimated economic loss of more than $6 billion a year to the local pig industry, when the body becomes infected with the two diseases. Will eventually be completely lost to immune function. Swine fever and pig blue ear disease belong to immunosuppressive disease because of their special pathogenic mechanism. The occurrence of immunosuppressive disease is the most important cause of the failure of immune prevention and control in pig farms. One of the factors of neglect. Therefore, in order to effectively prevent and control all kinds of swine diseases, it is urgent to control the occurrence of immunosuppressive diseases. At present, the two immunosuppressive diseases, swine fever and pig blue ear disease, are not yet unified. Therefore, it is urgent to find out a set of reasonable and effective immunization procedures for the two groups. In this study, 42 healthy unimmunized weaned piglets were selected and 36 of them were randomly divided into 3 groups. Each group was divided into 2 replicates and the other 6 were used as blank control. Group A was immunized with swine fever at intervals of 7 days and then immunized with swine blue ear disease (group B). Group C was immunized at the same time, and the blood samples were collected from the anterior vena cava at day 102030, 4050 and 6070, respectively, according to the blood index and the ratio of feed to weight. The secretion level of specific antibody in humoral immunity, the proliferative ability of T lymphocytes in cellular immunity and the secretion level of IFN- 緯 were compared and analyzed. Results the average leukocyte count and lymphocyte percentage in group A and C were significantly higher than those in group B (p0.01) and control group (p0.01). However, there was no significant difference in other indexes among the groups. The results showed that the ratio of feed to weight of group A was the lowest and that of group C was the second. The results of ELISA kit of the highest specific antibody in group B showed that the average blocking rate of swine fever antibody in group A reached a peak value about 70 days after immunization and was significantly higher than that in group B (p0.01). Group C (P 0.01) and control group (P 0.01), group B was the lowest (P 0.01); The average S- P value of the three experimental groups showed an increasing trend, reached the peak at the 40th day after immunization, and then tended to be stable. The differences between the experimental groups before and after immunization were very significant (p0.01). But there was no significant difference between groups (p0.05). The results showed that the average stimulation index of group A reached its peak value about 40 days after immunization and was significantly higher than that of group B (p0.01). Group C (p0.01) and control group (p0.01). The results showed that the average IFN- 緯 concentration of group A reached its peak on the 30th day after immunization and was significantly higher than that of group B (p0.01). And control group (p0.01). Compared with group C, there was no significant difference (p0.05). In conclusion, the same conclusion can be drawn from the blood index, the ratio of feed to weight, the level of humoral immunity and the level of cellular immunity. The immune effect of swine fever was better than that of swine blue ear disease after 7 days' interval. This conclusion provides a theoretical basis for the formulation of reasonable and effective immunization procedures for swine fever and swine blue ear disease in the future.
【學位授予單位】：沈陽農(nóng)業(yè)大學
【學位級別】：碩士
【學位授予年份】：2016
【分類號】：S858.28
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本文關(guān)鍵詞：豬瘟與豬藍耳病疫苗不同免疫程序?qū)γ庖咝Ч绊懙难芯?/strong> 出處：《沈陽農(nóng)業(yè)大學》2016年碩士論文 論文類型：學位論文
更多相關(guān)文章： 豬瘟 豬藍耳病 血液指標 體液免疫 細胞免疫
【摘要】：古典型豬瘟(CSF)是一種非常嚴重的豬病毒性傳染病,尤其會對人口較為稠密的以養(yǎng)豬為主的國家造成重大的經(jīng)濟損失。豬藍耳病(PRRS)是迄今為止全世界各地區(qū)范圍內(nèi)在經(jīng)濟方面上造成最大負面影響的豬傳染病之一,據(jù)美國相關(guān)部門統(tǒng)計,豬藍耳病每年對其本土養(yǎng)豬業(yè)保守估計造成超過約60億美元的經(jīng)濟財產(chǎn)損失。當機體感染這兩種疾病以后,最終都會由于免疫功能徹底喪失、繼發(fā)其他疫病混合感染而死。豬瘟和豬藍耳病由于其各自特殊的致病機理而同屬于免疫抑制類疾病。免疫抑制疾病的發(fā)生是造成各大養(yǎng)豬場免疫防控失敗最不容忽視的因素之一,因此要想做好各類豬病的有效防控,控制好免疫抑制疾病的發(fā)生才是當務之急。而當前對于豬瘟和豬藍耳病這兩種免疫抑制疾病尚未形成統(tǒng)一、良好的免疫程序。因此針對二者摸索出一套合理、有效的免疫程序刻不容緩。本試驗選取42頭健康未免疫的斷奶仔豬,將其中36頭隨機分為3組(A、B、C),每組設2個重復,另外6頭作為空白對照。A組先免疫豬瘟間隔7天后免疫豬藍耳病,B組反之,C組采取同時分點免疫。分別于免疫前以及免疫后的第10、20、30、40、50、60、70天進行前腔靜脈無菌采血,分別從血液指標、料重比、體液免疫中特異性抗體的分泌水平、細胞免疫中T淋巴細胞的增殖能力以及IFN-γ的分泌水平這五個方面進行比較分析,結(jié)果如下：①血液指標結(jié)果顯示A組和C組平均白細胞數(shù)和淋巴細胞比例分別極顯著高于B組(p0.01)和對照組(p0.01),而其他指標各組間均無顯著差異(p0.05)。②料重比結(jié)果A組最低,C組其次,B組最高。③特異性抗體的ELISA試劑盒檢測結(jié)果顯示A組豬瘟抗體平均阻斷率在免疫后第70天左右達到峰值并極顯著高于B組(p0.01)、C組(p0.01)和對照組(p0.01),實驗組中B組最低(p0.01)；三個實驗組豬藍耳病抗體平均S/P值均呈上升趨勢,在免疫后第40天左右達到峰值,之后走勢趨于平穩(wěn),實驗組免疫前后差異均極顯著(p0.01),但組間差異不顯著(p0.05),對照組無趨勢變化。④T淋巴細胞增值能力結(jié)果顯示A組平均刺激指數(shù)在免疫后第40天左右達到峰值并極顯著高于B組(p0.01)、C組(p0.01)和對照組(p0.01),實驗組中B組最低(p0.01)。⑤IFN-γ分泌水平結(jié)果顯示A組平均IFN-γ濃度在免疫后第30天左右達到峰值并極顯著高于B組(p0.01)和對照組(p0.01),與C組差異不顯著(p0.05)綜上所述,不論是從血液指標,還是從料重比、體液免疫水平以及細胞免疫水平上來看,均可以得出一致結(jié)論,即先免疫豬瘟,間隔7天后再免疫豬藍耳病的免疫效果較好,此結(jié)論為將來制定豬瘟、豬藍耳病合理有效的免疫程序提供了一定的理論依據(jù)。
[Abstract]:Classical swine fever (CSF) is a very serious swine viral disease. In particular, significant economic losses will be caused to more densely populated countries where pig breeding is the main source of disease. Pig Blue ear Disease (PRRSs). So far, it is one of the most economically negative swine infectious diseases in all regions of the world. According to relevant departments in the United States, blue ear disease causes an estimated economic loss of more than $6 billion a year to the local pig industry, when the body becomes infected with the two diseases. Will eventually be completely lost to immune function. Swine fever and pig blue ear disease belong to immunosuppressive disease because of their special pathogenic mechanism. The occurrence of immunosuppressive disease is the most important cause of the failure of immune prevention and control in pig farms. One of the factors of neglect. Therefore, in order to effectively prevent and control all kinds of swine diseases, it is urgent to control the occurrence of immunosuppressive diseases. At present, the two immunosuppressive diseases, swine fever and pig blue ear disease, are not yet unified. Therefore, it is urgent to find out a set of reasonable and effective immunization procedures for the two groups. In this study, 42 healthy unimmunized weaned piglets were selected and 36 of them were randomly divided into 3 groups. Each group was divided into 2 replicates and the other 6 were used as blank control. Group A was immunized with swine fever at intervals of 7 days and then immunized with swine blue ear disease (group B). Group C was immunized at the same time, and the blood samples were collected from the anterior vena cava at day 102030, 4050 and 6070, respectively, according to the blood index and the ratio of feed to weight. The secretion level of specific antibody in humoral immunity, the proliferative ability of T lymphocytes in cellular immunity and the secretion level of IFN- 緯 were compared and analyzed. Results the average leukocyte count and lymphocyte percentage in group A and C were significantly higher than those in group B (p0.01) and control group (p0.01). However, there was no significant difference in other indexes among the groups. The results showed that the ratio of feed to weight of group A was the lowest and that of group C was the second. The results of ELISA kit of the highest specific antibody in group B showed that the average blocking rate of swine fever antibody in group A reached a peak value about 70 days after immunization and was significantly higher than that in group B (p0.01). Group C (P 0.01) and control group (P 0.01), group B was the lowest (P 0.01); The average S- P value of the three experimental groups showed an increasing trend, reached the peak at the 40th day after immunization, and then tended to be stable. The differences between the experimental groups before and after immunization were very significant (p0.01). But there was no significant difference between groups (p0.05). The results showed that the average stimulation index of group A reached its peak value about 40 days after immunization and was significantly higher than that of group B (p0.01). Group C (p0.01) and control group (p0.01). The results showed that the average IFN- 緯 concentration of group A reached its peak on the 30th day after immunization and was significantly higher than that of group B (p0.01). And control group (p0.01). Compared with group C, there was no significant difference (p0.05). In conclusion, the same conclusion can be drawn from the blood index, the ratio of feed to weight, the level of humoral immunity and the level of cellular immunity. The immune effect of swine fever was better than that of swine blue ear disease after 7 days' interval. This conclusion provides a theoretical basis for the formulation of reasonable and effective immunization procedures for swine fever and swine blue ear disease in the future.
【學位授予單位】：沈陽農(nóng)業(yè)大學
【學位級別】：碩士
【學位授予年份】：2016
【分類號】：S858.28
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