本文關(guān)鍵詞：酵母培養(yǎng)物對綿羊瘤胃發(fā)酵及消化道內(nèi)營養(yǎng)物質(zhì)流通與消化的影響　出處：《吉林農(nóng)業(yè)大學(xué)》2016年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 酵母培養(yǎng)物 綿羊 瘤胃 揮發(fā)性脂肪酸 流通 消化

【摘要】：本文旨在通過體外模擬瘤胃法聯(lián)合體內(nèi)驗證試驗綜合評定篩選出酵母培養(yǎng)物(Yeast Culture,YC)對瘤胃發(fā)酵具有最佳效果的適宜添加量,并用該酵母培養(yǎng)物其最佳添加量與達(dá)農(nóng)威的益康XP比較,評價兩種酵母培養(yǎng)物對綿羊瘤胃發(fā)酵及消化道內(nèi)營養(yǎng)物質(zhì)流通與消化的影響的效果異同。本研究采用9只身體狀況良好、體重相近(35±2.67 kg),并裝有永久性瘤胃瘺管、十二指腸瘺管和回腸瘺管的小尾寒羊半同胞公羊作為實驗動物。論文共分為三個部分:首先,通過體外及體內(nèi)試驗,對本試驗所用YC與達(dá)農(nóng)威益康XP進(jìn)行比較,篩選出在綿羊日糧精粗比3:7條件下本試驗YC的適宜添加量。采用兩因素交叉分組試驗設(shè)計,試驗分為兩期:一期為體外試驗,試驗分為5組,對照組為不添加YC的基礎(chǔ)日糧,實驗II、III、IV組在基礎(chǔ)日糧上添加酵母培養(yǎng)物的劑量分別為1%、1.5%、2%,第V組在基礎(chǔ)日糧上添加2%的益康XP,每組三個重復(fù),依據(jù)試驗結(jié)果篩選出最佳的兩組添加劑量;二期為體內(nèi)試驗,試驗分為三組:對照組,體外篩選結(jié)果最佳的前兩個添加劑量組,每組三個重復(fù),體外及體內(nèi)均測定3個時間段的pH值、NH3-N、VFA濃度。結(jié)果表明:(1)試驗一:從整體趨勢看,III組、IV組與V組發(fā)酵效果最接近,能顯著降低NH3-N濃度、提高乙酸、丙酸、丁酸濃度(P0.05)。(2)試驗二:III組能顯著提高TVFA濃度、降低NH3-N濃度(P0.05),確定III組為綿羊的適宜添加量。其次,比較本試驗YC與益康XP對綿羊瘤胃發(fā)酵的影響。按試驗要求隨機分為3組,每組3個重復(fù),I組為對照組,II組投喂1.5%YC,III組投喂2%益康XP。結(jié)果表明:II組、III組與I組比較均能顯著提高pH值(P0.05)。II組NH3-N濃度顯著低與I組(P0.05),III組NH3-N濃度極顯著低于I組(P0.01),顯著低于II組(P0.05)。II組、III組均能極顯著提高總酸、乙酸、丙酸濃度(P0.01)。結(jié)果表明:添加1.5%酵母培養(yǎng)物能夠與添加2%益康XP對穩(wěn)定采食后瘤胃pH值、降低瘤胃NH3-N濃度、提高瘤胃TVFA濃度具有一致的顯著效果,并具有有提高瘤胃BCP濃度的趨勢。最后,比較本試驗YC與益康XP對綿羊消化道內(nèi)營養(yǎng)物質(zhì)流通與消化的影響。按試驗要求隨機分為3組,每組3個重復(fù),I組為對照組,II組投喂1.5%YC,III組投喂2%益康XP。結(jié)果表明:(1)兩種YC均降低了瘤胃液相流通速率、瘤胃液相體積以及滯留時間和DM(干物質(zhì))、OM(有機物)在直腸的流通量(P0.05),益康XP降低了ADF(酸性洗滌纖維)在直腸的流通量(P0.05)。(2)兩種YC均提高了瘤胃MN(微生物氮)流通量和十二指腸N(氮)的流通量,以及DM、OM、NDF(中性洗滌纖維)、ADF在全消化道的表觀消化率(P0.05),YC提高了CP在小腸的表觀消化率(P0.05)。結(jié)果表明,YC可能是通過提高營養(yǎng)物質(zhì)在小腸的表觀消化率,從而提高全消化道表觀消化率。
[Abstract]:The aim of this study was to select the optimal dosage of yeast culture YC for rumen fermentation by in vitro mimic rumen method combined with in vivo validation test. The optimum addition amount of the yeast culture was compared with the Yikang XP of Danongwei. To evaluate the effects of two yeast cultures on rumen fermentation and nutrient circulation and digestion in the digestive tract of sheep, 9 of them were in good physical condition with similar body weight of 35 鹵2.67 kg). The small tail Han sheep half-sibling ram with permanent rumen fistula duodenal fistula and ileal fistula were used as experimental animals. The thesis was divided into three parts: firstly the experiment was carried out in vitro and in vivo. By comparing YC with Danongwei Yikang XP, the suitable addition amount of YC was selected under the condition of 3: 7 forage ration of sheep diet. Two factors cross grouping experiment design was used. The experiment was divided into two periods: the first stage was in vitro trial, the experiment was divided into five groups, the control group was the basal diet without YC, and the experiment was IIHIII. The dosage of yeast culture added to the basal diet in group IV was 1: 1. 5 and 2 / 2 respectively. Group V added 2% of Yikang XP to the basal diet, which was repeated three times in each group. According to the test results, the best two groups of additives were selected. The second phase was in vivo trial. The experiment was divided into three groups: control group, the first two dosage groups with the best screening results in vitro, each group with three repetitions. The pH value of NH3-N was measured in vitro and in vivo. VFA concentration. The results showed that: 1. According to the overall trend, the fermentative effect of group IV and group V was the closest, which could significantly reduce the concentration of NH3-N and increase acetic acid and propionic acid. The concentration of butyric acid in group 2: III could significantly increase the concentration of TVFA and decrease the concentration of NH3-N (P0.05). Then, the effects of YC and Yikang XP on rumen fermentation of sheep were compared. According to the requirements of the experiment, the III group was randomly divided into 3 groups, each group with 3 repeats as the control group. Group II was fed 1.5yycyou III group and fed with 2% Yikang XP.The results showed that group II was fed with 1. 5% YCX III. Compared with group I, the concentration of NH3-N in III group was significantly higher than that in group I (P 0.05). The concentration of NH3-N in group II was significantly lower than that in group I (P 0.05). The concentration of NH3-N in group III was significantly lower than that in group I (P 0.01) and significantly lower than that in group II (P 0.05). Group II could significantly increase total acid and acetic acid. The results showed that 1.5% yeast culture and 2% Yikang XP could decrease the rumen pH value and reduce the rumen NH3-N concentration after feeding steadily. The increase of rumen TVFA concentration has a consistent and significant effect, and has the trend of increasing rumen BCP concentration. Finally. The effects of YC and Yikang XP on nutrient circulation and digestion in digestive tract of sheep were compared. III group was fed with 2% Yikang XP.The results showed that both YC and YC decreased rumen liquid flow rate, rumen liquid volume, retention time and DM (dry matter). Om (organic matter) flux in the rectum (P0.05). Yikang XP decreased the flux of ADF (acid detergent fiber) in the rectum (P0.05. 2) both YC and YC increased the flux of MN (microbial nitrogen) and duodenum (N) in the rumen. The flux. And the apparent digestibility of ADF in the whole digestive tract (P0.05). YC increased the apparent digestibility of CP in the small intestine. The results showed that YC could improve the apparent digestibility of the whole digestive tract by increasing the apparent digestibility of nutrients in the small intestine.
【學(xué)位授予單位】：吉林農(nóng)業(yè)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2016
【分類號】：S826.5

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 劉大程;劉國娟;盧春芳;劉月勝;祝琳敬;;體外法評價不同酵母菌發(fā)酵液對瘤胃發(fā)酵功能的影響[J];中國畜牧雜志;2015年01期

2 周淑芹;;酵母培養(yǎng)物對肉仔雞生長性能及免疫機能的影響研究[J];畜禽業(yè);2014年10期

3 冀紅芹;甄玉國;劉乙辰;趙小麗;陳雪;;不同促溶劑對酵母細(xì)胞在發(fā)酵液中自溶的影響[J];中國釀造;2014年05期

4 田書會;李根來;田文生;張遜;姚文;;酵母培養(yǎng)物對夏季生長育肥豬腸道菌群結(jié)構(gòu)和發(fā)酵能力的影響[J];南京農(nóng)業(yè)大學(xué)學(xué)報;2013年04期

5 楊平平;甄玉國;王曉磊;趙巍;于中英;;不同精粗比底物下不同添加劑對綿羊瘤胃體外發(fā)酵的影響[J];飼料工業(yè);2013年09期

6 肖曼;高振華;李興華;張少成;陳訓(xùn)銀;張曉慧;董愛華;曹贊;陳廣信;;酵母培養(yǎng)物對肉仔雞生長性能、腸黏膜結(jié)構(gòu)及腸道菌群的影響[J];動物營養(yǎng)學(xué)報;2013年07期

7 向浩;凌英會;張曉東;丁建平;章孝榮;;酵母培養(yǎng)物對安徽白山羊和波爾山羊生長發(fā)育的影響[J];中國草食動物科學(xué);2013年02期

8 肖曼;高振華;張少成;陳訓(xùn)銀;張曉慧;董愛華;陳廣信;曹贊;;酵母培養(yǎng)物對肉仔雞免疫功能、抗氧化及血清生化指標(biāo)的影響[J];廣東農(nóng)業(yè)科學(xué);2013年05期

9 李桉;郭雄全;李亞林;;復(fù)合酵母培養(yǎng)物對奶牛生產(chǎn)性能的影響[J];中國奶牛;2012年10期

10 寇慧娟;陳玉林;劉敬敏;曹寶紅;周光現(xiàn);張思慧;張恩平;;酵母培養(yǎng)物對羔羊生產(chǎn)性能、營養(yǎng)物質(zhì)表現(xiàn)消化率及瘤胃發(fā)育的影響[J];西北農(nóng)林科技大學(xué)學(xué)報(自然科學(xué)版);2011年08期

相關(guān)博士學(xué)位論文 前1條

1 蘇鵬程;不同代謝葡萄糖水平日糧條件下白絨山羊體內(nèi)蛋白質(zhì)（氨基酸）分配規(guī)律的研究[D];內(nèi)蒙古農(nóng)業(yè)大學(xué);2004年

相關(guān)碩士學(xué)位論文 前3條

1 關(guān)艷玲;酵母培養(yǎng)物對肉仔雞營養(yǎng)應(yīng)激緩解影響的研究[D];吉林農(nóng)業(yè)大學(xué);2008年

2 唐海翠;酵母培養(yǎng)物對瘤胃微生物體外發(fā)酵及山羊瘤胃代謝的影響[D];南京農(nóng)業(yè)大學(xué);2006年

3 沈美英;日糧內(nèi)不同粗飼料品質(zhì)對綿羊瘤胃發(fā)酵功能和微生物區(qū)系的影響[D];內(nèi)蒙古農(nóng)業(yè)大學(xué);2006年

，

本文編號：1359291