本文關(guān)鍵詞：雞冷誘導(dǎo)RNA結(jié)合蛋白基因序列分析及組織表達(dá)　出處：《動(dòng)物醫(yī)學(xué)進(jìn)展》2016年12期 　論文類型：期刊論文

  更多相關(guān)文章： 雞 冷誘導(dǎo)RNA結(jié)合蛋白 生物信息學(xué)分析 組織定量分布

【摘要】：為研究雞冷誘導(dǎo)RNA結(jié)合蛋白(CIRP)基因的結(jié)構(gòu)特征和組織定量分布情況,根據(jù)GenBank中原雞CIRP基因(NM-001031347)的編碼區(qū)序列自行設(shè)計(jì)引物,從健康雛雞脾臟中擴(kuò)增青腳麻羽雞CIRP基因的編碼區(qū)(CDS)并進(jìn)行生物信息學(xué)分析;設(shè)計(jì)引物建立實(shí)時(shí)熒光定量RT-PCR,檢測(cè)CIRP mRNA在雛雞各組織器官中的分布及相對(duì)表達(dá)水平。結(jié)果顯示,雞CIRP基因CDS區(qū)全長(zhǎng)573bp,編碼190個(gè)氨基酸,其氨基端包含一個(gè)RNA結(jié)合域,羧基端富含RGG重復(fù)序列,推導(dǎo)的多肽鏈羧基端比鴨、斑胸草雀及哺乳動(dòng)物要多18個(gè)氨基酸;CIRP基因在雞各被檢組織器官中均有表達(dá),相對(duì)表達(dá)水平由低到高分別為法氏囊、肺臟、胰腺、胸腺、脾臟、睪丸、盲腸扁桃體、心臟、腦、腎臟、哈德氏腺、肝臟。本研究為進(jìn)一步探討雞CIRP基因的遺傳進(jìn)化及其在病原感染中的作用提供了參考。
[Abstract]:To study the structural characteristics and tissue quantitative distribution of cold induced RNA binding protein (CIRP) gene in chicken. Primers were designed according to the coding region sequence of CIRP gene NM-001031347 in GenBank. The coding region of CIRP gene was amplified from the spleen of healthy chicken and analyzed by bioinformatics. Real-time fluorescent quantitative RT-PCRs were designed to detect the distribution and relative expression of CIRP mRNA in chicken tissues and organs. Chicken CIRP gene CDS region is 573 BP, encoding 190 amino acids, its amino terminal contains a RNA binding domain, carboxyl terminal is rich in RGG repeat sequence, deduced polypeptide chain carboxyl ratio duck. The tit and mammal had more than 18 amino acids. CIRP gene was expressed in all tissues and organs of chicken, and the relative expression levels from low to high were bursa of Fabricius, lung, pancreas, thymus, spleen, testis, cecum tonsil, heart, brain and kidney. This study provides a reference for further study on the genetic evolution of chicken CIRP gene and its role in pathogenic infection.
【作者單位】： 成都農(nóng)業(yè)科技職業(yè)學(xué)院;西南民族大學(xué)生命科學(xué)與技術(shù)學(xué)院;
【基金】：四川省教育廳創(chuàng)新團(tuán)隊(duì)項(xiàng)目(13TD0057)
【分類號(hào)】：S831
【正文快照】： 冷誘導(dǎo)RNA結(jié)合蛋白(cold-inducible RNAbinding protein,CIRP或CIRBP)是一個(gè)18ku的蛋白,屬于RNA結(jié)合蛋白異源核糖核蛋白亞群[1]。CIRP最初是在研究紫外線輻射引起DNA損傷的轉(zhuǎn)錄反應(yīng)時(shí)發(fā)現(xiàn)的,它以一種劑量依賴增殖方式參與對(duì)紫外線輻射的轉(zhuǎn)錄應(yīng)答[2-3],隨后Nishiyama H等[4]又，

本文編號(hào)：1356161