【摘要】：C型利尿鈉肽對小鼠Cajal間質(zhì)細胞起搏電流的影響 1893年，西班牙神經(jīng)解剖學家cajial用甲基藍及嗜銀染色法在胃腸道神經(jīng)系統(tǒng)(enteric nervous system，ENS)中觀察到一類特殊的間質(zhì)細胞，命名為Cajal間質(zhì)細胞(interstitial cells of Cajal，ICC)。ICC是ENS中的一種非神經(jīng)但與神經(jīng)有關(guān)的特殊間質(zhì)細胞，胃腸道平滑肌的運動形式在很大程度上取決于ICC的活動。已有眾多研究表明，許多胃腸道平滑肌的靜息電位不穩(wěn)定，表現(xiàn)為緩慢的起伏波動，，即周期性去極化和復極化，稱為慢波(slow wave，SW)，也稱基本電節(jié)律(Basic Electric Rhythm，BER)。BER決定著平滑肌收縮節(jié)律，調(diào)控著胃腸道運動發(fā)生的時間、地點、頻率、和方向。近年來的單細胞記錄和分子水平的研究證明，胃腸道無論是縱行肌還是環(huán)行肌均不具有發(fā)動SW的能力。慢波由存在于縱行肌和環(huán)行肌之間的ICC產(chǎn)生，這些細胞具有成纖維細胞和平滑肌細胞的特性，并與縱環(huán)兩層形成縫隙連接，可將慢波傳到平滑肌。近年的許多研究表明，ICC是腸道慢波的起搏者，在神經(jīng)肌肉信號傳遞中起調(diào)節(jié)作用，而且與某些胃腸動力性疾病的發(fā)病有關(guān)。 利尿鈉肽(natriuretic peptides)家族是一類具有利尿、排鈉，舒張血管，降低血壓以及調(diào)節(jié)水鹽和電解質(zhì)平衡等作用的多肽。利尿鈉肽家族的受體有3種，分別是A型、B型和C型利尿鈉肽受體(natriuretic peptides receptors，NPR)。利尿鈉肽通過與其受體結(jié)合而發(fā)揮作用。CNP作為利尿鈉肽家族中的一員，是由Sudoh等人于1990年首先在豬的大腦中發(fā)現(xiàn)的，是一種具有22個氨基酸殘基的肽類：CNP分布廣泛，存在于中樞神經(jīng)系統(tǒng)、心血管系統(tǒng)、泌尿生殖系統(tǒng)、消化系統(tǒng)以及皮膚和粘膜等，幾乎遍布于整個機體。CNP作為一種局部調(diào)節(jié)因子通過自分泌和旁分泌的方式發(fā)揮作用，除了可以利尿、排鈉，舒張血管和內(nèi)臟平滑肌，降低血壓以及調(diào)節(jié)水鹽、電解質(zhì)平衡以外，還可以抑制血管平滑肌的增殖 調(diào)節(jié)腎素血管緊張素的活性和調(diào)節(jié)腸液和膽汁等消化液的分泌。此外，還參與高血壓、慢性心衰、慢性腎衰、動脈粥樣硬化和肺纖維化等一系列病理過程的調(diào)節(jié)。CNP的許多作用是通過調(diào)節(jié)細胞膜上的離子通道，影響細胞內(nèi)信號轉(zhuǎn)導來實現(xiàn)的。 我室以往的研究表明：在大鼠胃的各個部位都有利尿鈉肽受體(natriuretic
[Abstract]:Effects of C-type diuretic natriuretic peptide on pacing current of mouse Cajal interstitial cells in 1893, Cajial, a Spanish neuroanatomist, observed a special type of interstitial cells in the gastrointestinal (enteric nervous system by methylene blue and argyrophilic staining. Cajal interstitial cells (interstitial cells of Cajal ICC). ICC is a kind of special interstitial cells in ENS, which is non-nerve but related to nerve. The motility of gastrointestinal smooth muscle depends on the activity of ICC to a great extent. Many studies have shown that the resting potential of gastrointestinal smooth muscle is unstable, which is characterized by slow fluctuation, that is, periodic depolarization and repolarization, known as slow wave SW, also known as basic electrical rhythm (Basic Electric Rhythm-BER, which determines the contraction rhythm of smooth muscle. It regulates the time, place, frequency, and direction of gastrointestinal movements. In recent years, single cell recording and molecular studies have proved that gastrointestinal tract, whether longitudinal or circular, does not have the ability to initiate SW. The slow waves are produced by ICC which exists between longitudinal and circular muscles. These cells possess the characteristics of fibroblasts and smooth muscle cells and form a gap junction with the longitudinal ring to transmit the slow waves to the smooth muscle. In recent years, many studies have shown that ICC is a slow wave pacemaker, which plays a regulatory role in neuromuscular signal transduction, and is related to the pathogenesis of some gastrointestinal motility diseases. Diuretic natriuretic peptide (natriuretic peptides) family is a family of polypeptides with diuretic, natriuretic, vasodilating, lowering blood pressure, and regulating the balance of water, salt and electrolyte. There are three kinds of diuretic natriuretic peptide receptor, type A, type B, and type C, (natriuretic peptides receptor. Diuretic natriuretic peptide acts by binding to its receptor. CNP, as a member of the diuretic natriuretic peptide family, was first discovered in the pig brain by Sudoh et al in 1990. Present in the central nervous system, cardiovascular system, genitourinary system, digestive system, skin and mucous membrane, almost all over the body. CNP acts as a local regulatory factor through autocrine and paracrine. In addition to diuretic, sodium excretion, vasodilator and visceral smooth muscle, lowering blood pressure and regulating water, salt, electrolyte balance, It can also inhibit the proliferation of vascular smooth muscle, regulate the activity of renin angiotensin and regulate the secretion of digestive fluid such as intestinal fluid and bile. In addition, CNP is involved in the regulation of a series of pathological processes such as hypertension, chronic heart failure, chronic renal failure, atherosclerosis and pulmonary fibrosis. Previous studies in my laboratory have shown that diuretic natriuretic peptide receptors (natriuretic) are present in all parts of the rat stomach.
【學位授予單位】：延邊大學
【學位級別】：碩士
【學位授予年份】：2006
【分類號】：R33

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