【摘要】： 第一部分放大倍數(shù)和包埋介質(zhì)對睪丸組織形態(tài)定量研究的影響 研究背景與目的: 形態(tài)定量研究中,我們通常利用組織切片,根據(jù)體視學(xué)原理測量切片上觀察到的圖像,由此估計組織結(jié)構(gòu)的形態(tài)定量特征。由于切片受取材、固定、脫水、包埋、切片、裱片和染色以及顯微分辨率和放大倍數(shù)等諸多因素的影響,不同條件下測得的結(jié)果可能不同。這是一個基本而又重要的問題,但可能是因為影響因素(包括測量方法)太多,對這個問題的系統(tǒng)研究或綜述相對很少。筆者實驗室以前對比過國產(chǎn)石蠟與環(huán)氧樹脂包埋對睪丸組織形態(tài)定量研究的影響,最近又對比了國際上常用的Leica石蠟和甲基丙烯酸樹脂包埋以及放大倍數(shù)對腎臟組織形態(tài)定量研究的影響。本文擬進一步對比Leica石蠟和甲基丙烯酸樹脂包埋以及不同物鏡(不同分辨率和放大倍數(shù))對睪丸組織結(jié)構(gòu)形態(tài)定量研究的影響。 方法: 動物:6只正常成年(8.6~9.6月齡)雄性新西蘭大耳白兔。麻醉后取每只兔的一側(cè)睪丸(3只取自左側(cè),3只取自右側(cè))。經(jīng)Bouin液
[Abstract]:Part one: the influence of magnification and embedding medium on the quantitative study of testicular tissue morphology: background and objective: In quantitative morphological studies, We usually use tissue sections to measure the images observed on the slices according to the stereology principle, and then estimate the morphological and quantitative characteristics of the tissue structure. Due to the influence of many factors, such as material selection, fixation, dehydration, embedding, slicing, laminating and dyeing, microresolution and magnification, the results may be different under different conditions. This is a basic and important problem, but it may be because there are so many influential factors (including measurement methods) that there is relatively little systematic study or review of the problem. The authors have compared the effects of paraffin wax and epoxy resin embedding on the quantitative study of testicular morphology in our laboratory. The effects of Leica paraffin wax and methacrylic resin embedding and magnification on the quantitative study of renal tissue morphology were also compared recently. The effects of Leica paraffin wax and methacrylic resin embedding and different objective lenses (different resolution and magnification) on the quantitative study of testicular tissue morphology were further compared in this paper. Methods: six normal adult (8.6 ~ 9.6 months old) male New Zealand white rabbits were used. After anesthesia, the testis were taken from one side of each rabbit (3 from the left side and 3 from the right). Via Bouin solution
【學(xué)位授予單位】：重慶醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2006
【分類號】：R329

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本文編號：2155300