【摘要】：重組乙型肝炎表面抗原(HBsAg)疫苗是在世界范圍內(nèi)廣泛應(yīng)用的預(yù)防乙型肝炎病毒的有效疫苗,但在我國(guó)約10%的人群對(duì)其反應(yīng)低下或無(wú)反應(yīng),因此研制一種高效的乙型肝炎疫苗已成為當(dāng)務(wù)之急。 CpG ODN 是人工合成的含有CpG 的單鏈寡聚脫氧核苷酸,具有強(qiáng)效免疫增強(qiáng)作用。CpG ODN 可分為A、B、C 三類,其中B 類CpG ODN(CpG-B)能有效刺激B 細(xì)胞介導(dǎo)的體液免疫應(yīng)答,可望成為增強(qiáng)HBsAg 免疫效果的理想分子佐劑。 為了尋找能夠有效增強(qiáng)HBsAg 免疫原性的CpG ODN,我們進(jìn)行了大規(guī)模的新型CpG ODN 篩選工作,發(fā)現(xiàn)一種新型的CpG ODN(CpG-684)能夠刺激淋巴細(xì)胞增殖、活化、刺激IFN-γ的產(chǎn)生,且能明顯提高HBsAg刺激小鼠產(chǎn)生抗體的能力。說(shuō)明這種新型CpG ODN 有可能成為乙肝疫苗的高效佐劑。
[Abstract]:Recombinant hepatitis B surface antigen (HBsAg) vaccine is an effective vaccine for the prevention of hepatitis B virus in the world, but about 10% of the people in our country have low response or no response to it. Therefore, it has become an urgent task to develop a highly effective hepatitis B vaccine.
CpG ODN is a synthetic single strand oligo deoxynucleotides containing CpG, which can be divided into three classes of A, B, C, which can be divided into three classes of A, B, and C. The B class CpG ODN (CpG-B) can effectively stimulate the humoral immune response mediated by the cells. It is expected to be a molecular adjuvant to enhance the immune effect.
In order to find CpG ODN which can effectively enhance the immunogenicity of HBsAg, we carried out a large-scale new CpG ODN screening work. It was found that a new type of CpG ODN (CpG-684) can stimulate lymphocyte proliferation, activation, stimulate the production of IFN- gamma, and can obviously improve the ability of HBsAg stimulates mice to produce antibodies. This new CpG ODN is available. It can be an efficient adjuvant for hepatitis B vaccine.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2005
【分類號(hào)】：R392.1

【共引文獻(xiàn)】

相關(guān)期刊論文 前10條

1 盛學(xué)岐,林存智,朱新紅;CD1a分子和IFN-γ因子對(duì)良惡性胸腔積液診斷價(jià)值[J];青島大學(xué)醫(yī)學(xué)院學(xué)報(bào);2005年04期

2 譚文杰;阮力;;HBV病毒慢性感染疫苗治療的組合策略及研究進(jìn)展[J];病毒學(xué)報(bào);2006年05期

3 武廣恒;張家穎;楊麗紅;周樂(lè);;非特異性角膜炎癥反應(yīng)中PDTC對(duì)LPS誘導(dǎo)LFA-1 mRNA表達(dá)的影響[J];吉林大學(xué)學(xué)報(bào)(醫(yī)學(xué)版);2007年04期

4 婁玉鈐;劉雅敏;劉健;;熱痹清片誘導(dǎo)AA大鼠滑膜細(xì)胞凋亡的作用及機(jī)制[J];中華中醫(yī)藥雜志;2007年09期

5 蔣玉紅;王亞秋;孫寬周;;支氣管哮喘患者TH細(xì)胞的極化狀態(tài)及臨床意義[J];中國(guó)當(dāng)代兒科雜志;2006年05期

6 蔣玉紅;劉凱;王濤;王亞秋;;鉛中毒兒童外周血T淋巴細(xì)胞免疫活性改變及臨床意義[J];中國(guó)兒童保健雜志;2006年05期

7 劉雯,李守新;類風(fēng)濕關(guān)節(jié)炎成纖維樣滑膜細(xì)胞凋亡研究進(jìn)展[J];中華風(fēng)濕病學(xué)雜志;2005年10期

8 伍小軍;潘志忠;方m錁，

本文編號(hào)：2140097