發(fā)布時(shí)間：2018-07-11 15:16

  本文選題：C57BL/6 + 日本血吸蟲��； 參考：《南京醫(yī)科大學(xué)》2006年碩士論文

【摘要】：目前，血吸蟲病的防治工作強(qiáng)調(diào)以化療為主，結(jié)合螺螄控制、健康教育等手段來降低血吸蟲病的危害。雖然，化療在我國實(shí)施過程中起到了很好作用，但面臨著再感染及抗藥性等問題。鑒于疫苗在預(yù)防和消滅許多傳染性疾病中發(fā)揮的重要作用，發(fā)展血吸蟲病疫苗，從而使化療“短效”作用與疫苗接種后產(chǎn)生的“長效”免疫預(yù)防作用相結(jié)合來控制血吸蟲病，成為上個(gè)世紀(jì)80年代以來全世界科學(xué)家們的共識。 減毒活疫苗如輻照致弱血吸蟲尾蚴可在多種動(dòng)物模型中誘導(dǎo)出高保護(hù)力，然而，，由于涉及諸如抗原材料來源有限、制備、保存和安全性等問題，導(dǎo)致了輻照致弱尾蚴應(yīng)用的受限。研究者們希望通過揭示輻照致弱尾蚴誘導(dǎo)保護(hù)力的免疫機(jī)制，從而為新疫苗的設(shè)計(jì)提供理論依據(jù)。 對曼氏血吸蟲輻照致弱尾蚴誘導(dǎo)保護(hù)力和相關(guān)免疫應(yīng)答機(jī)制的研究較多，其常用的小鼠品系有：C57BL／6、DBA、CBA等，其中，C57BL／6為一高應(yīng)答品系。與曼氏血吸蟲相比，日本血吸蟲輻照致弱尾蚴在小動(dòng)物(特別是小鼠)中誘導(dǎo)產(chǎn)生的保護(hù)力水平常不穩(wěn)定，且目前缺乏公認(rèn)的能對其產(chǎn)生穩(wěn)定高應(yīng)答的小鼠模型。 目前，國內(nèi)外對輻照致弱尾蚴的相關(guān)免疫學(xué)研究多集中在曼氏血吸蟲，對日本血吸蟲的研究則起步較晚，多在借鑒曼氏血吸蟲病的相關(guān)研究經(jīng)驗(yàn)的基礎(chǔ)上開展。因此，構(gòu)建對日本血吸蟲輻照致弱尾蚴具有高保護(hù)力的小鼠模型，并動(dòng)態(tài)觀察日本血吸蟲輻照致弱尾蚴誘導(dǎo)的免疫應(yīng)答狀況，對于探索輻照致弱尾蚴誘導(dǎo)保護(hù)力的免疫學(xué)機(jī)制至關(guān)
[Abstract]:At present, the prevention and control of schistosomiasis emphasizes chemotherapy mainly, combined with snail control, health education and other means to reduce the harm of schistosomiasis. Although chemotherapy has played a very good role in the implementation of China, it is facing the problems of reinfection and resistance to drug. In view of the importance of vaccine in the prevention and elimination of many infectious diseases The development of schistosomiasis vaccine, which makes the "short effect" effect of chemotherapy combined with the "long effect" immunization after vaccination to control schistosomiasis, has become the consensus of scientists all over the world since the 80s of the last century.
Attenuated live vaccines, such as irradiated weak schistosoma cercariae, can induce high protection in a variety of animal models. However, problems such as limited source of antigen, preparation, preservation and safety have led to the limitation of the application of irradiated cercariae. Researchers hope to pass through the immune system that reveals the protective ability of irradiated cercariae. It provides a theoretical basis for the design of the new vaccine.
There are many studies on the protective and related immune response mechanisms of cercariae induced by Schistosoma mansoni irradiation. The commonly used mice lines are C57BL / 6, DBA, CBA and so on. Among them, C57BL / 6 is a high response strain. Compared with Schistosoma mansoni, the protections induced by Schistosoma japonicum in small animals (especially mice) are induced by the irradiation of Schistosoma mansoni The level is often unstable, and there is no commonly accepted mouse model that can produce stable and high response to it.
At present, the related immunological studies of irradiated cercariae at home and abroad are mostly concentrated on Schistosoma mansoni, and the research on Schistosoma japonicum is relatively late, and it is based on the related research experience of mansoni schistosomiasis. Therefore, a mouse model with high protective ability to irradiate the weak cercariae caused by Schistosoma japonicum is constructed and the dynamic view is established. Observing the immune response induced by irradiated cercariae weakened by Schistosoma japonicum, it is very important to explore the immunological mechanism of radiation induced weak cercariae.
【學(xué)位授予單位】：南京醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2006
【分類號】：R383;R392

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