本文選題：骨形態(tài)發(fā)生蛋白 + 轉(zhuǎn)化生長因子β　； 參考：《東北農(nóng)業(yè)大學(xué)》2005年碩士論文

【摘要】：本實驗應(yīng)用不同濃度的鹽酸胍和尿素反復(fù)溶解沉淀蛋白質(zhì)的方法從牛骨中分離制備骨形態(tài)發(fā)生蛋白(BMP),以及應(yīng)用酸醇法從豬血小板中提取制備轉(zhuǎn)化生長因子β(TGF-β),并將制備的樣品植入小鼠股部肌肉內(nèi)進(jìn)行誘導(dǎo)成骨實驗,旨在探索BMP誘導(dǎo)成骨的最佳方式。實驗結(jié)果如下: (1) 相同條件下,使用載體比無載體具有更強(qiáng)的誘導(dǎo)能力,合適載體的使用會使誘導(dǎo)實驗取得更好的效果; (2) BMP與TGF-β聯(lián)合使用比單獨(dú)使用BMP具有更好的誘導(dǎo)成骨效果,表明BMP的誘導(dǎo)只是啟動過程,TGF-β可促進(jìn)誘導(dǎo)物進(jìn)一步分化增殖; (3) 從載體組的誘導(dǎo)使用可以看出,新骨形成后,其堿性磷酸酶的活性降低,表明此時新生細(xì)胞表達(dá)減弱,合成有機(jī)質(zhì)降低;而從鈣的變化可看出,此時更多的是無機(jī)質(zhì)的形成,即骨中鈣鹽的沉積; (4) 通過細(xì)胞生物學(xué)實驗同樣證明BMP與TGF-β聯(lián)合使用比單獨(dú)使用BMP具有更好的誘導(dǎo)成骨效果,而且可以驗證骨的修復(fù)再生來源于間充質(zhì)干細(xì)胞。 綜上所述,BMP誘導(dǎo)成骨的最佳條件是,使用合適的材料作為載體,同時與其他生長因子聯(lián)合應(yīng)用。
[Abstract]:In this experiment, bone morphogenetic protein (BMP) was isolated from bovine bone with different concentrations of guanidine hydrochloride and urea, and transforming growth factor 尾 (TGF- 尾) was extracted from porcine platelets by acid alcohol method. The samples were implanted into the femoral muscles of mice for osteoinduction. The aim of this study was to explore the best method of osteogenesis induced by BMP. The experimental results are as follows: (1) under the same conditions, the use of the carrier has a stronger induction ability than the non-carrier. (2) the combination of BMP and TGF- 尾 has better osteogenic effect than that of BMP alone. The results indicated that BMP induction was only a priming process, and TGF- 尾 could promote the further differentiation and proliferation of the inducer. (3) from the induction of carrier group, we can see that after the formation of new bone, The activity of alkaline phosphatase was decreased, which indicated that the expression of new cells decreased and the synthesis of organic matter decreased, but the change of calcium showed that the formation of inorganic substance was more likely at this time. (4) the combined use of BMP and TGF- 尾 also proved that BMP and TGF- 尾 could induce osteogenesis better than BMP alone. And it can be proved that bone repair and regeneration come from mesenchymal stem cells. To sum up, the best condition of BMP osteoinduction is to use suitable materials as carrier and combined with other growth factors.
【學(xué)位授予單位】：東北農(nóng)業(yè)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2005
【分類號】：R33;S857.16

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 余家闊，曲綿域，田得祥，王健全，張美榮，宋衛(wèi)明，劉燕，李文;載體對haMp活性影響的實驗研究[J];中華外科雜志;1996年10期

2 ;骨及骨骼生理學(xué)[J];國外科技資料目錄(醫(yī)藥衛(wèi)生);1999年04期

3 蔣雷生;;吸附于羥基磷灰石的骨形態(tài)發(fā)生蛋白的骨膜下植入[J];國際骨科學(xué)雜志;1992年01期

4 李雙良;骨形態(tài)發(fā)生蛋白研究進(jìn)展[J];中國實驗動物學(xué)雜志;1996年04期

5 原野,張明偉,于曉Y，

本文編號：2110725