發(fā)布時間：2018-06-26 20:02

  本文選題：Jagged + 破骨細胞��； 參考：《中國免疫學雜志》2014年07期

【摘要】：目的:探討Jagged1通過活化Notch通路對RAW 264.7細胞向破骨分化及增殖的影響。方法:將RAW 264.7細胞分三組培養(yǎng):對照組(細胞培養(yǎng)基+鼠RANKL 50 ng/L)、Jagged1組(細胞培養(yǎng)基+鼠RANKL+Jagged1重組蛋白)、γ-分泌酶抑制劑(DAPT)組(細胞培養(yǎng)基+鼠RANKL+Jagged1重組蛋白+DAPT),實時熒光定量PCR檢測各組破骨細胞標志基因組織蛋白酶K(Cathepsin K,CK)、抗酒石酸性磷酸酶(Tartrate-resistant acid phosphatase,TRAP)、降鈣素受體(Calcitionin receptor,CTR)及Notch靶基因HES-1、HEY-1 mRNA的表達,TRAP染色鑒定破骨細胞,掃描電鏡檢測破骨細胞溶骨功能。免疫熒光檢測NICD的表達變化,CCK-8檢測RAW 264.7細胞增殖。結果:Jagged1組TRAP、CK、CTR及HES-1、HEY-1 mRNA的表達、TRAP+細胞數(shù)較對照組及DAPT組明顯升高(P0.05),而DAPT組與對照組均無明顯變化;細胞免疫熒光顯示Jagged1組NICD除表達于細胞膜、細胞質(zhì)外,細胞核也有較高表達,對照組及DAPT組細胞核中NICD無明顯表達;細胞培養(yǎng)48 h,Jagged1組細增殖較對照組及DAPT組出現(xiàn)明顯抑制。結論:Jagged1通過活化Notch通路促進RAW 264.7向破骨細胞分化、抑制其增殖。
[Abstract]:Aim: to investigate the effect of JaggeD1 on osteoclast differentiation and proliferation of raw 264.7 cells by activating Notch pathway. Methods: raw 264.7 cells were cultured in three groups: the control group (RANKL 50 ng / L) and the 緯 -secretory enzyme inhibitor group (RANKL Jagged1 recombinant protein) and the 緯 -secretory enzyme inhibitor group (RANKL Jagged1 recombinant protein DAPT). The expression of cathepsin K (CK), tartrate-resistant acid phosphatase trap (trap), calcitonin receptor (CTR) and Notch target gene HES-1hHEY-1 mRNA in osteoclasts were detected by fluorescence quantitative polymerase chain reaction (FQ-PCR). The osteolytic function of osteoclasts was examined by scanning electron microscope. The expression of NICD was detected by immunofluorescence and CCK-8 was used to detect the proliferation of raw 264.7 cells. Results compared with control group and DAPT group, the number of TRAPCKCTR and HES-1pHEY-1 mRNA expression of TRAPCKCTR and HES-1HEY-1 mRNA in group 1 were significantly higher than those in control group and DAPT group (P0.05), but there were no significant changes in DAPT group and control group, and cell immunofluorescence showed that NICD in Jagged1 group was expressed not only in cell membrane, but also in cytoplasm. The expression of NICD in the nuclei of control group and DAPT group was not obvious, and the fine proliferation of JaggeD1 group was significantly inhibited than that of control group and DAPT group. Conclusion by activating Notch pathway, Jagged1 promotes the differentiation of raw 264.7 into osteoclasts and inhibits its proliferation.
【作者單位】： 第三軍醫(yī)大學第二附屬醫(yī)院骨科;
【基金】：國家自然科學基金面上項目(No.81271979)
【分類號】：R392

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